Mouse NAD(P)H dehydrogenase [quinone] 1 (NQO1) ELISA kit

Code CSB-EL016039MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
NAD(P)H dehydrogenase, quinone 1
Alternative Names
Nqo1 ELISA Kit; Dia4 ELISA Kit; Nmo1 ELISA Kit; Nmor1 ELISA Kit; NAD(P)H dehydrogenase [quinone] 1 ELISA Kit; EC 1.6.5.2 ELISA Kit; Azoreductase ELISA Kit; DT-diaphorase ELISA Kit; DTD ELISA Kit; Menadione reductase ELISA Kit; NAD(P)H:quinone oxidoreductase 1 ELISA Kit; Phylloquinone reductase ELISA Kit; Quinone reductase 1 ELISA Kit; QR1 ELISA Kit
Abbreviation
NQO1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse NQO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %97
Range %92-104
1:2Average %94
Range %88-97
1:4Average %84
Range %80-88
1:8Average %89
Range %83-97
Recovery
The recovery of mouse NQO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9386-98
EDTA plasma (n=4)9994-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.705 2.600 2.653 2.498
10002.304 2.371 2.338 2.183
5001.659 1.758 1.709 1.554
2500.794 0.795 0.795 0.640
1250.495 0.522 0.509 0.354
62.50.352 0.357 0.355 0.200
31.250.207 0.224 0.216 0.061
00.150 0.160 0.155  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse NQO1 ELISA Kit was designed for the quantitative measurement of Mouse NQO1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

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Target Background

Function
(From Uniprot)
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Gene References into Functions
  1. Although the exact role of NQO1 in prostatic hyperplasia remains unclear, the hyperplasia exacerbation due to NQO1 deletion might be independent of type 2 5alpha-reductase and might be related to enhanced androgen receptor affinity due to enhanced HSP90-alpha expression. PMID: 29110315
  2. Novel RNA-binding activity of NQO1 promotes SERPINA1 mRNA translation. PMID: 27515817
  3. NQO1 and autophagy participate in a protective role in cisplatin injury. PMID: 26935540
  4. Miroestrol restored hepatic NQO1 expression in beta-naphthoflavone-treated mice. PMID: 26893163
  5. previous and present results collectively show that NQO1 is involved in the formation of tight junctions in the small intestine, and that defects in NQO1 enhance C. difficile toxin A-induced acute inflammatory responses, presumably via the loss of epithelial cell tight junctions PMID: 27116994
  6. NQO1 plays a critical role in protection against energy depletion in acetaminophen-induced liver injury, and is associated with improvement of mitochondrial dysfunction PMID: 25224400
  7. The present results demonstrate that exacerbated cisplatin-induced nephrotoxicity under the NQO1-knockout condition was accompanied by the reduced expression of MRN complex proteins, ATM, PARP1, and Sirt1. PMID: 26723870
  8. We defined the basal and butylated hydroxyanisole induced expression patterns of Nqo1, AKR1B8, and Ho-1 in the liver and small intestine of C57BL/6 mice. PMID: 26291391
  9. The colons of NQO1-KO mice also showed high levels of reactive oxygen species (ROS) and histone deacetylase (HDAC) activity, which are known to affect transcriptional regulation. PMID: 24393524
  10. induction by humulusol PMID: 24397993
  11. the induction of cellular NAD(+) levels using beta-lapachone (beta-Lap), whose intracellular target is NQO1, prevents the toxic effects of cisplatin through the regulation of PARP-1 and SIRT1 activity. PMID: 24922076
  12. Nqo1 expression is protective against renal ischemia/reperfusion injury in mice. PMID: 24189322
  13. results indicate that AAI can increase its own metabolic activation by inducing NQO1, thereby enhancing its own genotoxic potential. PMID: 24769487
  14. PCB-77 induced reductions in insulin signaling in adipose tissue were also abolished by Resveratrol, which increased NQO1 expression. PMID: 24231106
  15. NQO1 does not play a major role in the production of vitamin K hydroquinone and supports the existence of multiple vitamin K reduction pathways. PMID: 24015818
  16. NQO1 protects cells against renal failure induced by cisplatin; this effect is mediated by decreased NOX activity via cellular NADPH/NADP modulation. PMID: 23831944
  17. findings link NQO1, a cellular redox sensor, to the metabolite-sensing network that tunes PGC-1alpha expression and activity in regulating energy metabolism PMID: 23648480
  18. In the present study we show that [a] NQO1 protein locates to cytosol, ER and mitochondria. PMID: 23692925
  19. the results demonstrate for the first time that NQO1 is an endogenous factor in protection against painful bladder syndrome PMID: 22985937
  20. NADPH:quinone oxidoreductase 1 regulates host susceptibility to ozone via isoprostane generation PMID: 23275341
  21. hypertrophy is negatively regulated by NQO1 activity mouse 3T3-L1 adipocytes. PMID: 22683604
  22. NAD(P)H quinone oxidoreductase 1 regulates neutrophil elastase-induced mucous cell metaplasia. PMID: 22659878
  23. expression in cochlea did not decrease with age PMID: 21631178
  24. PGI synthase overexpression protects against bleomycin-induced mortality and is associated with increased Nqo 1 expression. PMID: 21764988
  25. NQO1 plays an important role in renal AAI nitroreduction and may thus contribute to AAI-induced nephrotoxicity. PMID: 21613233
  26. catalase overexpression upregulates BaP-induced NQO1 expression by enhancing the Sp1-AhR-Nrf2 signaling cascade. PMID: 21569840
  27. NQO1 activation has a hypotensive effect mediated by eNOS activation via cellular NAD(+)/NADH ratio modulation in an animal model. PMID: 21502369
  28. Hyperhomocysteinemia leads to a decrease of hepatic Nqo1 gene expression and activity with a reversal effect of wine polyphenol extract supplementation. PMID: 21550378
  29. Plasma membrane electron transport in pancreatic beta-cells is mediated in part by NQO1. PMID: 21505151
  30. findings assign NQO1 an original role in the regulation of mRNA translation via the control of eukaryotic translation initiation factor 4GI stability by the proteasome. PMID: 20028737
  31. NQO1 regulates p53 proteasomal degradation independently of MDM2 and ubiquitin. PMID: 12232053
  32. Induction of NQO1 activity exaggerates neuronal damage in a mouse focal cerebral ischaemia model PMID: 12603827
  33. NQO1 has an important role in stabilizing hot-spot p53 mutant proteins in cancer PMID: 14634213
  34. A role is suggested for NQO1 in myelination in the central nervous system or in the insulating/wrapping function of glial cells. PMID: 14991562
  35. These results indicate that one division of both the accessory and the main olfactory projection maps are composed of sensory neurons that are specialized to reduce environmental and/or endogenously produced quinones via an NQO1-dependent mechanism. PMID: 15128404
  36. NQO1 has a role in regulating ubiquitin-independent degradation of ornithine decarboxylase by the 20S proteasome PMID: 15749015
  37. This is the first demonstration that heavy metals (mercury, lead and copper) regulate the expression of Noqo1 gene through a transcriptonal mechanism. PMID: 16243960
  38. NQO1 plays a pivotal role in signaling activated by TNF and other inflammatory stimuli and that its suppression is a potential therapeutic strategy for tumor cells. PMID: 16682409
  39. NQO1 and NQO2 are endogenous factors in the regulation of immune response and autoimmunity PMID: 16905546
  40. NOS2 induction and NO production may be involved in TNF-alpha- and LPS-mediated downregulation of Cyp1a1 without affecting the downregulation of Nqo1. PMID: 17588258
  41. A major in vivo role of NQO1 in metabolic activation of mitomycin C with implications in mitomycin C chemotherapy. PMID: 17804703
  42. Bach1 repression of ferritin and thioredoxin reductase1 is heme-sensitive in cells and in vitro and coordinates expression with heme oxygenase1, beta-globin, and NADP(H) quinone (oxido) reductase1. PMID: 17901053
  43. Drug metabolizing enzyme induction of Nqo1 is reported in experimental fatty liver. PMID: 18488193
  44. The lack of induction of apoptosis and differentiation contributed to radiation-induced myeloproliferative disease in NQO1(-/-) mice. PMID: 18829548
  45. NQO1 modulates cellular redox status and influences the biologic and physiologic effects of ozone. PMID: 19059883
  46. It is concluded that cisplatin markedly increases the sensitivity of cancer to beta-lap in vitro and in vivo by upregulating NQO1. PMID: 19738461

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Subcellular Location
Cytoplasm.
Protein Families
NAD(P)H dehydrogenase (quinone) family
Database Links

KEGG: mmu:18104

STRING: 10090.ENSMUSP00000003947

UniGene: Mm.252

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