Mouse Secreted frizzled-related protein 4(SFRP4) ELISA kit

Code CSB-EL021140MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
secreted frizzled-related protein 4
Alternative Names
Sfrp4 ELISA Kit; Secreted frizzled-related sequence protein 4 ELISA Kit; FRP-4 ELISA Kit; sFRP-4 ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.47 ng/mL-30 ng/mL
0.11 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Stem Cells
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SFRP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %89
Range %84-92
1:2Average %100
Range %95-105
1:4Average %88
Range %84-93
1:8Average %107
Range %104-110
The recovery of mouse SFRP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9390-96
EDTA plasma (n=4)8985-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
302.809 2.869 2.839 2.631
152.231 2.268 2.250 2.042
7.51.514 1.567 1.541 1.333
3.750.845 0.800 0.823 0.615
1.870.585 0.575 0.580 0.372
0.940.462 0.469 0.466 0.258
0.470.342 0.344 0.343 0.135
00.209 0.207 0.208
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse SFRP4 ELISA Kit was designed for the quantitative measurement of Mouse SFRP4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.47 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL.

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Target Background

(From Uniprot)
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
Gene References into Functions
  1. SFRP4 expression levels were gradually increased and proportionally associated with epididymal white adipose tissue adipocyte differentiation toward maturation at 14 days, while inguinal white adipose tissue adipocyte just showed an opposite tendency. PMID: 29730658
  2. Results indicate that sFRP4 plays a critical role in bone development and remodeling by regulating osteoblasts and osteoclasts, and that its functional loss prevents age-related bone loss in the trabecular bone. These findings imply that it functions as a potential balancer of the Wnt signaling pathway by direct influencing bone formation and absorption during skeletal bone development and maintenance through remodeling. PMID: 27117872
  3. the cross-talk between SFRP4, integrin alpha1beta1, and Notch1 suppresses the cardiac differentiation of P19CL6 cells. PMID: 27542621
  4. Our study showed that Pyle's disease was caused by a deficiency of sFRP4 and that sFRP4-mediated cross-regulation between Wnt and BMP signaling was critical for achieving proper cortical-bone thickness and stability. PMID: 27355534
  5. MicroRNA-124 regulates cell specification in the cochlea through modulation of Sfrp4 and Sfrp5. PMID: 26387953
  6. In diet-induced obesity, loss of SFRP4 alters body length and bone mineral density as well as energy expenditure and food intake. However, SFRP4 does not control glucose homeostasis and beta-cell mass in mice. PMID: 26406932
  7. MG-derived oxidative stress (not CpG demethylation) epigenetically and rapidly derepress sFRP-4 gene expression. PMID: 25036934
  8. findings suggest that an epigenetic event is critically involved in the pathogenesis of psoriasis, and the downregulation of SFRP4 by CpG island methylation is one possible mechanism contributing to the hyperplasia of epidermis in the disease PMID: 25825452
  9. extra-follicular modulators Bmp2, Dkk1, and Sfrp4 increase in early anagen. PMID: 24618599
  10. Data suggest that Sfrp4 does not contribute to the long-term regulation of serum phosphate levels in mice. PMID: 21427221
  11. sFRP4 negatively regulates bone formation without disrupting phosphorus homeostasis. PMID: 20472109
  12. the first promoter analysis of mouse Frp genes providing the basis for understanding the functions and the regulation of Frp and its role in regulating Wnt signals PMID: 12974383
  13. beta-catenin signaling is activated in tubular epithelial and interstitial cells after renal injury, and recombinant sFRP4 interferes with epithelial de-differentiation and fibroblast differentiation and function during progression of renal fibrosis PMID: 15944336
  14. Sfrp4 negatively regulates bone formation and decreases BMD through the inhibition of Wnt signaling PMID: 17002585
  15. These results indicate that Sfrp4 decreases bone formation at least in part by attenuating canonical Wnt signaling in vivo. PMID: 17907918
  16. SFRP4 may modulate Wnt functions in Tsk skin fibrosis. PMID: 17943183

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Subcellular Location
Protein Families
Secreted frizzled-related protein (sFRP) family
Tissue Specificity
Expressed in the ovary. Localized to granulosa cells of periovulatory follicles and corpora lutea. Weakly expressed in adult tissues including kidney, brain and lung.
Database Links
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