Mouse Secreted frizzled-related protein 4(SFRP4) ELISA kit

Product Details

Customer Reviews and Q&A

Target Background

Product Details

Target Name

secreted frizzled-related protein 4

Alternative Names

Sfrp4 ELISA Kit; Secreted frizzled-related sequence protein 4 ELISA Kit; FRP-4 ELISA Kit; sFRP-4 ELISA Kit

Abbreviation

SFRP4

Uniprot No.

Q9Z1N6

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.47 ng/mL-30 ng/mL

Sensitivity

0.11 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Stem Cells

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SFRP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-92
1:2	Average %	100
	Range %	95-105
1:4	Average %	88
	Range %	84-93
1:8	Average %	107
	Range %	104-110

Recovery

The recovery of mouse SFRP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	89	85-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.809	2.869	2.839	2.631
15	2.231	2.268	2.250	2.042
7.5	1.514	1.567	1.541	1.333
3.75	0.845	0.800	0.823	0.615
1.87	0.585	0.575	0.580	0.372
0.94	0.462	0.469	0.466	0.258
0.47	0.342	0.344	0.343	0.135
0	0.209	0.207	0.208

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse SFRP4 ELISA Kit was designed for the quantitative measurement of Mouse SFRP4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.47 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL.

Target Background

Function
(From Uniprot)

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.

Gene References into Functions

SFRP4 expression levels were gradually increased and proportionally associated with epididymal white adipose tissue adipocyte differentiation toward maturation at 14 days, while inguinal white adipose tissue adipocyte just showed an opposite tendency. PMID: 29730658
Results indicate that sFRP4 plays a critical role in bone development and remodeling by regulating osteoblasts and osteoclasts, and that its functional loss prevents age-related bone loss in the trabecular bone. These findings imply that it functions as a potential balancer of the Wnt signaling pathway by direct influencing bone formation and absorption during skeletal bone development and maintenance through remodeling. PMID: 27117872
the cross-talk between SFRP4, integrin alpha1beta1, and Notch1 suppresses the cardiac differentiation of P19CL6 cells. PMID: 27542621
Our study showed that Pyle's disease was caused by a deficiency of sFRP4 and that sFRP4-mediated cross-regulation between Wnt and BMP signaling was critical for achieving proper cortical-bone thickness and stability. PMID: 27355534
MicroRNA-124 regulates cell specification in the cochlea through modulation of Sfrp4 and Sfrp5. PMID: 26387953
In diet-induced obesity, loss of SFRP4 alters body length and bone mineral density as well as energy expenditure and food intake. However, SFRP4 does not control glucose homeostasis and beta-cell mass in mice. PMID: 26406932
MG-derived oxidative stress (not CpG demethylation) epigenetically and rapidly derepress sFRP-4 gene expression. PMID: 25036934
findings suggest that an epigenetic event is critically involved in the pathogenesis of psoriasis, and the downregulation of SFRP4 by CpG island methylation is one possible mechanism contributing to the hyperplasia of epidermis in the disease PMID: 25825452
extra-follicular modulators Bmp2, Dkk1, and Sfrp4 increase in early anagen. PMID: 24618599
Data suggest that Sfrp4 does not contribute to the long-term regulation of serum phosphate levels in mice. PMID: 21427221
sFRP4 negatively regulates bone formation without disrupting phosphorus homeostasis. PMID: 20472109
the first promoter analysis of mouse Frp genes providing the basis for understanding the functions and the regulation of Frp and its role in regulating Wnt signals PMID: 12974383
beta-catenin signaling is activated in tubular epithelial and interstitial cells after renal injury, and recombinant sFRP4 interferes with epithelial de-differentiation and fibroblast differentiation and function during progression of renal fibrosis PMID: 15944336
Sfrp4 negatively regulates bone formation and decreases BMD through the inhibition of Wnt signaling PMID: 17002585
These results indicate that Sfrp4 decreases bone formation at least in part by attenuating canonical Wnt signaling in vivo. PMID: 17907918
SFRP4 may modulate Wnt functions in Tsk skin fibrosis. PMID: 17943183

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Subcellular Location

Secreted.

Protein Families

Secreted frizzled-related protein (sFRP) family

Tissue Specificity

Expressed in the ovary. Localized to granulosa cells of periovulatory follicles and corpora lutea. Weakly expressed in adult tissues including kidney, brain and lung.

Database Links

KEGG: mmu:20379

STRING: 10090.ENSMUSP00000002883

UniGene: Mm.42095

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Code	CSB-EL021140MO
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.