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Mouse Spondin-1(SPON1) ELISA kit

Mouse Spondin-1(SPON1) ELISA kit

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Code	CSB-EL022599MO
Size	96T,5×96T,10×96T
Trial Size
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Target Background

Product Details

Target Name

spondin 1, extracellular matrix protein

Alternative Names

Spon1 ELISA Kit; Spondin-1 ELISA Kit; F-spondin ELISA Kit

Abbreviation

SPON1

Uniprot No.

Q8VCC9

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	97-106
1:2	Average %	104
	Range %	99-108
1:4	Average %	86
	Range %	81-92
1:8	Average %	94
	Range %	89-98

Recovery

The recovery of mouse SPON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-106
EDTA plasma (n=4)	87	83-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.249	2.342	2.296	2.142
5	1.472	1.587	1.530	1.376
2.5	0.925	0.964	0.945	0.791
1.25	0.591	0.607	0.599	0.445
0.625	0.392	0.386	0.389	0.235
0.312	0.301	0.311	0.306	0.152
0.156	0.218	0.222	0.220	0.066
0	0.153	0.154	0.154

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

5-7 working days

Citations

SPON1 Can Reduce Amyloid Beta and Reverse Cognitive Impairment and Memory Dysfunction in Alzheimer’s Disease Mouse Model SY Park,Cells,2020

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Target Background

Function (From Uniprot)	Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity).
Gene References into Functions	We conclude that upon ligand binding, TLR4 activates PI3K/Akt signaling to induce F-spondin expression, subsequently control CREB-mediated IL-6 production to promote VSMC migration. These findings provide vital insights into the essential role of F-spondin in VSMC function and will be valuable for developing new therapeutic strategies against atherosclerosis. PMID: 27091427 These findings demonstrated novel roles for F-spondin and IL-1Ra in type B CpG oligodeoxynucleotides-mediated cell proliferation and migration of macrophages. PMID: 26042735 Spon1 deletion leads to a local and systemic reduction of TGF-beta levels resulting in increased BMP signaling and increased bone deposition in adult mice. PMID: 24875054 F-spondin is expressed in embryonic cartilage, where it has the capacity to enhance chondrocyte terminal differentiation and mineralization. PMID: 20839318
Subcellular Location	Secreted, extracellular space, extracellular matrix
Database Links	KEGG: mmu:233744 STRING: 10090.ENSMUSP00000041157 UniGene: Mm.334160

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