Mouse Spondin-1(SPON1) ELISA kit

Instructions
Code CSB-EL022599MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name spondin 1, extracellular matrix protein
Alternative Names Spon1 ELISA Kit; Spondin-1 ELISA Kit; F-spondin ELISA Kit
Abbreviation SPON1
Uniprot No. Q8VCC9
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 102
Range % 97-106
1:2 Average % 104
Range % 99-108
1:4 Average % 86
Range % 81-92
1:8 Average % 94
Range % 89-98
Recovery
The recovery of mouse SPON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 97-106
EDTA plasma (n=4) 87 83-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.249 2.342 2.296 2.142
5 1.472 1.587 1.530 1.376
2.5 0.925 0.964 0.945 0.791
1.25 0.591 0.607 0.599 0.445
0.625 0.392 0.386 0.389 0.235
0.312 0.301 0.311 0.306 0.152
0.156 0.218 0.222 0.220 0.066
0 0.153 0.154 0.154  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity).
Gene References into Functions
  1. We conclude that upon ligand binding, TLR4 activates PI3K/Akt signaling to induce F-spondin expression, subsequently control CREB-mediated IL-6 production to promote VSMC migration. These findings provide vital insights into the essential role of F-spondin in VSMC function and will be valuable for developing new therapeutic strategies against atherosclerosis. PMID: 27091427
  2. These findings demonstrated novel roles for F-spondin and IL-1Ra in type B CpG oligodeoxynucleotides-mediated cell proliferation and migration of macrophages. PMID: 26042735
  3. Spon1 deletion leads to a local and systemic reduction of TGF-beta levels resulting in increased BMP signaling and increased bone deposition in adult mice. PMID: 24875054
  4. F-spondin is expressed in embryonic cartilage, where it has the capacity to enhance chondrocyte terminal differentiation and mineralization. PMID: 20839318
Subcellular Location Secreted, extracellular space, extracellular matrix
Database Links

KEGG: mmu:233744

STRING: 10090.ENSMUSP00000041157

UniGene: Mm.334160

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