Mouse myeloid-derived growth factor precursor (C19orf10) ELISA kit

Instructions
Code CSB-EL003433MO
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chromosome 19 open reading frame 10
Alternative Names Mydgf ELISA Kit; D17Wsu104e ELISA Kit; Myeloid-derived growth factor ELISA Kit; MYDGF ELISA Kit
Abbreviation C19orf10
Uniprot No. Q9CPT4
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 90    
Range % 87-93    
1:2 Average % 104    
Range % 100-107    
1:4 Average % 89    
Range % 85-93    
1:8 Average % 102    
Range % 98-106    
Recovery
The recovery of mouse IL25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 88 84-93    
EDTA plasma (n=4) 102 97-108    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
\\\"\\\"
pg/ml OD1 OD2 Average Corrected    
1000 2.680 2.598 2.639 2.502    
500 1.877 1.902 1.890 1.753    
250 1.153 1.204 1.179 1.042    
125 0.749 0.738 0.744 0.607    
62.5 0.408 0.419 0.414 0.277    
31.2 0.342 0.339 0.341 0.204    
15.6 0.267 0.261 0.264 0.127    
0 0.136 0.137 0.137      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway.
Gene References into Functions
  1. There were no significant differences in clinical scores and numbers of infiltrating eosinophils among IL-25KO, TSLPR KO, and wild-type mice. PMID: 26244295
  2. Mydgf-deficient mice develop larger infarct scars and more severe contractile dysfunction compared to wild-type mice after experimental myocardial infarction. PMID: 25581518
Subcellular Location Secreted, Endoplasmic reticulum-Golgi intermediate compartment
Protein Families MYDGF family
Tissue Specificity Expressed in prostate, spleen and lung, and weakly expressed in the left ventricle (LF) and liver. Expressed predominantly in inflammatory cells, such as monocytes and macrophages, and weakly expressed in neutrophils, T-cells, B-cells, endothelial cells a
Database Links

KEGG: mmu:28106

UniGene: Mm.294870

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