Rabbit procollagen Ⅰ N-terminal peptide(PⅠNP)ELISA Kit

Code CSB-E17085Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rabbit PⅠNP ELISA Kit was designed for the quantitative measurement of Rabbit PⅠNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .

Target Name procollagen Ⅰ N-terminal peptide(PⅠNP)
Alternative Names
procollagen 1 N-terminal peptide(P1NP),procollagen I N-terminal peptide(PINP)
Abbreviation PⅠNP
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-92  
1:2 Average % 96  
Range % 91-105  
1:4 Average % 103  
Range % 92-110  
1:8 Average % 93  
Range % 84-98  
The recovery of rabbit PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 89 82-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.843 2.763 2.803 2.721  
25 2.304 2.128 2.216 2.134  
12.5 1.577 1.475 1.526 1.444  
6.25 0.909 0.899 0.904 0.822  
3.12 0.426 0.421 0.424 0.342  
1.56 0.213 0.206 0.210 0.128  
0.78 0.147 0.140 0.144 0.062  
0 0.084 0.080 0.082    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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