Product Details

Target Name

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6

Alternative Names

Serpina6 ELISA kit; CbgCorticosteroid-binding globulin ELISA kit; CBG ELISA kit; Serpin A6 ELISA kit; Transcortin ELISA kit

Abbreviation

SERPINA6

Uniprot No.

Q06770

Species

Mus musculus (Mouse)

Sample Types

serum, plasma

Detection Range

0.2 μg/mL-80 μg/mL

Sensitivity

0.2 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPINA6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-102
1:2	Average %	95
	Range %	87-105
1:4	Average %	91
	Range %	83-99
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of mouse SERPINA6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	86-105
EDTA plasma (n=4)	96	89-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
0	2.135	2.121	2.128
0.2	1.528	1.498	1.513
0.7	0.992	1.013	1.003
2.5	0.544	0.552	0.548
15	0.339	0.352	0.346
80	0.160	0.157	0.159

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse SERPINA6 ELISA Kit was designed for the quantitative measurement of Mouse SERPINA6 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.2 μg/mL-80 μg/mL and the sensitivity is 0.2 μg/mL.

Target Background

Function
(From Uniprot)

Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.

Gene References into Functions

CBG is involved in the gender differences observed in corticosteroids circulating levels and plays a role in the local regulation of corticosteroids availability in organs like lung. PMID: 26741814
the same gene encodes brain and liver corticosteroid-binding globulin PMID: 24246737
Data suggest fine control interaction between plasma CBG upregulation and stress-induced corticosterone (CORT) release in mouse model of psychological stress; CBG appears to function in maintaining dynamic equilibrium between CBG-bound/-unbound CORT. PMID: 24048966
Sequencing analysis showed that Cbg cDNA obtained from the mouse hypothalamus was homologous to Cbg cDNA obtained from the liver. PMID: 22996440
Cbg-/- mice, unlike controls, remain insensitive to stress applied before memory retrieval. Furthermore, under stress conditions, we observed a blunted surge of corticosterone (CORT) in plasma and no free CORT rise in the hippocampus of Cbg-/-. PMID: 22930537
study describes functional hyporesponsiveness and aldosterone resistance in distal colon of CBG(-/-) mice; steroid actions require an intrinsic but unknown function of CBG, which allows the sufficient supply of the hormone/s to the target tissue PMID: 20969731
Critical role of plasma Cbg in providing an adequate endocrine and behavioral response to stress. PMID: 20022933
These data suggest an active role for CBG in bioavailability, local delivery, and/or cellular signal transduction of glucocorticoids that extends beyond a function as a mere cargo transporter. PMID: 16980625

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Subcellular Location

Secreted.

Protein Families

Serpin family

Tissue Specificity

Expressed by the liver; secreted in plasma.

Database Links

KEGG: mmu:12401

STRING: 10090.ENSMUSP00000044033

UniGene: Mm.290079

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Code	CSB-EL021062MO
Size	96T,5×96T,10×96T
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Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Mouse Corticosteroid-binding globulin(SERPINA6) ELISA kit

Product Details

Customer Reviews and Q&A

Target Background