Flow Cytometry is a technique used for rapid, multi-parameter analysis of individual cells or other biological particles, which can be used for immunophenotyping, cell cycle analysis, apoptosis detection, cell function analysis, and cell sorting.
The detection methods of flow cytometry are mainly categorized into direct detection and indirect detection. Direct detection uses fluorescently labeled antibodies, such as fluorescein isothiocyanate (FITC), phycoerythrin (PE), phycocyanin (APC), etc., which directly bind to the target molecules and emit fluorescent signals at specific wavelengths under the laser irradiation of the flow cytometer, thus realizing quantitative analysis of cells. Indirect detection, on the other hand, utilizes unlabeled primary antibodies to bind to target molecules, which are then detected by fluorescently labeled secondary antibodies. This method increases the sensitivity of the assay, and is especially suitable for the detection of low-abundance antigens.
The quality of the antibody directly affects the flow cytometry results. CUSABIO provides a variety of high-quality fluorescent labeled flow antibodies, unlabeled flow antibodies, fluorescent labeled secondary antibodies and isotype control antibodies to meet your different choices in flow experiments.
THP-1 cells (1:250)
HL-60 cells (1:250)
Hela cells (1:100)
HepG2 cells (1:100)
Jurkat cells (1:250)
Raji cells (1:250)
Jurkat cells (1:100)
RAW264.7 cells (1:100)
Product Name | Code | Species Reactivity | Fluorescent labeling | Applications |
---|---|---|---|---|
CD14 Monoclonal Antibody | CSB-MA214986 | Human | FITC | ELISA,IF,FC |
CD14 Monoclonal Antibody | CSB-MA804053 | Human | PE | ELISA,IF,FC |
CD14 Monoclonal Antibody | CSB-MA072336 | Human | PE-Cy5 | ELISA,IF,FC |
CD14 Monoclonal Antibody | CSB-MA263985 | Human | APC | ELISA,IF,FC |
CD19 Monoclonal Antibody | CSB-MA036486 | Human | PE | ELISA,IF,FC |
CD19 Monoclonal Antibody | CSB-MA047662 | Human | FITC | ELISA,IF,FC |
CD19 Monoclonal Antibody | CSB-MA281869 | Human | PE-Cy5 | ELISA,IF,FC |
CD19 Monoclonal Antibody | CSB-MA195220 | Human | APC | ELISA,FC |
CD2 Monoclonal Antibody | CSB-MA785710 | Human | FITC | ELISA,IF,FC |
CD34 Monoclonal Antibody | CSB-MA989061 | Human | PE | ELISA,IF,FC |
CD3E Monoclonal Antibody | CSB-MA566015 | Human | FITC/PE/PE-Cy5 | ELISA,IF,FC |
CD3E Monoclonal Antibody | CSB-MA920656 | Human | FITC/PE | ELISA,IF,FC |
CD3E Monoclonal Antibody | CSB-MA252451 | Human | FITC/PE | ELISA,IF,FC |
CD3E Monoclonal Antibody | CSB-MA782605 | Human | FITC/PE | ELISA,IF,FC |
CD3E Monoclonal Antibody | CSB-MA087211 | Human | FITC/PE | ELISA,IF,FC |
CD3E Monoclonal Antibody | CSB-MA157951 | Human | FITC/PE | ELISA,IF,FC |
CD3E Monoclonal Antibody | CSB-MA270849 | Human | FITC | ELISA,IF,FC |
CD3E Monoclonal Antibody | CSB-MA634374 | Human | PE | ELISA,IF,FC |
CD3E Monoclonal Antibody | CSB-MA843517 | Human | PE-Cy5 | ELISA,IF,FC |
CD3E Monoclonal Antibody | CSB-MA028904 | Human | APC | ELISA,IF,FC |
Product Name | Code | Target | Species Reactivity | Applications |
---|---|---|---|---|
ABCB1 Monoclonal Antibody | CSB-MA001046A0m | ABCB1 | Human | ELISA, WB, IF, FC |
DARC Recombinant Monoclonal Antibody | CSB-RA287686A0HU | ACKR1 | Human | ELISA, FC |
ACLY Monoclonal Antibody | CSB-MA177768 | ACLY | Human,Mouse,Rat,Pig | ELISA,WB,ICC,FC |
ACLY Recombinant Monoclonal Antibody | CSB-RA712206A0HU | ACLY | Human | ELISA, WB, IF, FC |
ACO1 Recombinant Monoclonal Antibody | CSB-RA298469A0HU | ACO1 | Human | ELISA, WB, IF, FC |
ACTA1 Recombinant Monoclonal Antibody | CSB-RA289956A0HU | ACTA1 | Human | ELISA, IHC, FC |
ACTA1 Recombinant Monoclonal Antibody | CSB-RA001205A0HU | ACTA1 | Human, Mouse, Rat | ELISA, WB, IHC, IF, FC |
ACTB Monoclonal Antibody | CSB-MA000091M1m | ACTB | Human, Mouse, Rat, Rabbit | ELISA, WB, IHC, IF, FC |
ADAM10 Recombinant Monoclonal Antibody | CSB-RA001273MA1HU | ADAM10 | Human | ELISA, FC |
AGO2 Recombinant Monoclonal Antibody | CSB-RA965823A0HU | AGO2 | Human | ELISA, IF, FC |
AKR1C3 Recombinant Monoclonal Antibody | CSB-RA825204A0HU | AKR1C3 | Human | ELISA, IF, FC |
AKT1/2/3 Recombinant Monoclonal Antibody | CSB-RA272392A0HU | AKT1/2/3 | Human | ELISA, WB, IHC, FC |
ALB Recombinant Monoclonal Antibody | CSB-RA264109A0HU | ALB | Human | ELISA, FC |
ALPP Recombinant Monoclonal Antibody | CSB-RA183247A0HU | ALPP | Human | ELISA, WB, IHC, FC |
ANPEP Recombinant Monoclonal Antibody | CSB-RA001827MA1HU | ANPEP | Human | ELISA, IF, FC |
ANXA1 Recombinant Monoclonal Antibody | CSB-RA001836MA1HU | ANXA1 | Human | ELISA, IHC, FC |
ANXA4 Recombinant Monoclonal Antibody | CSB-RA001845MA1HU | ANXA4 | Human, Mouse | ELISA, WB, FC |
APOA2 Recombinant Monoclonal Antibody | CSB-RA001915A0HU | APOA2 | Human | ELISA, WB, IHC, FC |
APOL1 Recombinant Monoclonal Antibody | CSB-RA966671A0HU | APOL1 | Human | ELISA, FC |
ARG2 Recombinant Monoclonal Antibody | CSB-RA216908A0HU | ARG2 | Human | ELISA, IHC, IF, FC |
Product name | Code | Fluorescent labeling | Application |
---|---|---|---|
IgG1 Monoclonal Antibody | CSB-MA255382 | FITC | FC |
IgG1 Monoclonal Antibody | CSB-MA792617 | PE | FC |
IgG2a Monoclonal Antibody | CSB-MA081360 | FITC | ELISA,IF,FC |
IgG2a Monoclonal Antibody | CSB-MA072925 | PE | ELISA,IF,FC |
IgG2b Monoclonal Antibody | CSB-MA993519 | FITC | ELISA,IF,FC |
IgG2b Monoclonal Antibody | CSB-MA982879 | PE | ELISA,IF,FC |
Product name | Code | Fluorescent labeling | Application |
---|---|---|---|
Goat Anti-Mouse IgG(H+L) Antibody | CSB-PA980279 | FITC | IHC,IF,FC |
Goat Anti-Rabbit IgG(H+L) Antibody | CSB-PA340892 | FITC | IHC,IF,FC |
Goat Anti-Human IgG, Fcγ fragment specific | CSB-PA271513 | FITC | FC |
Q: Why is high background in flow cytometry (FC)?
a. Too high gain set. You should reduce the gain to decrease the signal and use the positive control to set up the flow cytometer correctly again.
b. Too much antibody. You should decrease the antibody concentration.
Q: Why are there two or more cell populations observed in flow cytometry (FC)?
a. More than one cell population expressing target protein. You should check if there is adequate cell separation.
b. Cell doublets present. Doublets of cells will show as a second cell population at approximately twice the fluorescence intensity on the plot. You should mix cells gently using a pipette before staining and before running on the cytometer.
Q: How should antibodies be selected in double-labeled immunofluorescence?
When performing double-labeling immunofluorescence experiments, the selection of appropriate antibodies is crucial for observing the co-localization of two antigenic proteins in cells. Below are the antibody selection options for different situations:
a. Two directly labeled antibodies: If available, two directly labeled primary antibodies with different fluorescent markers should be selected. This reduces the risk of cross-reactivity of the secondary antibodies and is easier to handle.
b. Two indirectly labeled antibodies: In this case, two primary antibodies with different host sources (Host), each paired with a different secondary antibody with different fluorescent markers, need to be selected to avoid cross-reactivity.
c. One direct-labeled antibody plus one indirectly labeled antibody: If one is from a direct-labeled primary antibody and the other is unlabeled, then a primary antibody of a different host source and the corresponding secondary antibody can be selected. Both antibodies can be incubated at the same time because the directly labeled primary antibody will not cross-react with the secondary antibody of the other primary antibody.
d. Two indirectly labeled antibodies of the same host origin: If the two primary antibodies are of the same host origin, it is necessary to incubate one primary antibody and use the corresponding secondary antibody for detection, then incubate the second primary antibody and use its corresponding secondary antibody. This avoids cross-reactivity between secondary antibodies of the same host source.