Flow Cytometry Antibodies

Flow Cytometry Antibodies

Flow Cytometry is a technique used for rapid, multi-parameter analysis of individual cells or other biological particles, which can be used for immunophenotyping, cell cycle analysis, apoptosis detection, cell function analysis, and cell sorting.

The detection methods of flow cytometry are mainly categorized into direct detection and indirect detection. Direct detection uses fluorescently labeled antibodies, such as fluorescein isothiocyanate (FITC), phycoerythrin (PE), phycocyanin (APC), etc., which directly bind to the target molecules and emit fluorescent signals at specific wavelengths under the laser irradiation of the flow cytometer, thus realizing quantitative analysis of cells. Indirect detection, on the other hand, utilizes unlabeled primary antibodies to bind to target molecules, which are then detected by fluorescently labeled secondary antibodies. This method increases the sensitivity of the assay, and is especially suitable for the detection of low-abundance antigens.

The quality of the antibody directly affects the flow cytometry results. CUSABIO provides a variety of high-quality fluorescent labeled flow antibodies, unlabeled flow antibodies, fluorescent labeled secondary antibodies and isotype control antibodies to meet your different choices in flow experiments.

Product Advantages:

High specificity
High sensitivity
High stability
Multiple cell validation
Multiple application validation

Some of the Featured Products:

CD31 Monoclonal Antibody
Application:ELISA, WB, IHC, IF, FC

THP-1 cells (1:250)


HL-60 cells (1:250)

PKM Monoclonal Antibody
Application:ELISA, WB, IHC, IF, FC

Hela cells (1:100)


HepG2 cells (1:100)

CD45 Monoclonal Antibody
Application:ELISA, WB, IHC, IF, FC

Jurkat cells (1:250)


Raji cells (1:250)

CD14 Monoclonal Antibody
Application:ELISA, WB, IF, FC

Jurkat cells (1:100)


RAW264.7 cells (1:100)

Fluorescently Labeled Primary Antibodies:

Product Name Code Species Reactivity Fluorescent labeling Applications
CD14 Monoclonal Antibody CSB-MA214986 Human FITC ELISA,IF,FC
CD14 Monoclonal Antibody CSB-MA804053 Human PE ELISA,IF,FC
CD14 Monoclonal Antibody CSB-MA072336 Human PE-Cy5 ELISA,IF,FC
CD14 Monoclonal Antibody CSB-MA263985 Human APC ELISA,IF,FC
CD19 Monoclonal Antibody CSB-MA036486 Human PE ELISA,IF,FC
CD19 Monoclonal Antibody CSB-MA047662 Human FITC ELISA,IF,FC
CD19 Monoclonal Antibody CSB-MA281869 Human PE-Cy5 ELISA,IF,FC
CD19 Monoclonal Antibody CSB-MA195220 Human APC ELISA,FC
CD2 Monoclonal Antibody CSB-MA785710 Human FITC ELISA,IF,FC
CD34 Monoclonal Antibody CSB-MA989061 Human PE ELISA,IF,FC
CD3E Monoclonal Antibody CSB-MA566015 Human FITC/PE/PE-Cy5 ELISA,IF,FC
CD3E Monoclonal Antibody CSB-MA920656 Human FITC/PE ELISA,IF,FC
CD3E Monoclonal Antibody CSB-MA252451 Human FITC/PE ELISA,IF,FC
CD3E Monoclonal Antibody CSB-MA782605 Human FITC/PE ELISA,IF,FC
CD3E Monoclonal Antibody CSB-MA087211 Human FITC/PE ELISA,IF,FC
CD3E Monoclonal Antibody CSB-MA157951 Human FITC/PE ELISA,IF,FC
CD3E Monoclonal Antibody CSB-MA270849 Human FITC ELISA,IF,FC
CD3E Monoclonal Antibody CSB-MA634374 Human PE ELISA,IF,FC
CD3E Monoclonal Antibody CSB-MA843517 Human PE-Cy5 ELISA,IF,FC
CD3E Monoclonal Antibody CSB-MA028904 Human APC ELISA,IF,FC

Unlabeled Flow-through Antibodies:

Product Name Code Target Species Reactivity Applications
ABCB1 Monoclonal Antibody CSB-MA001046A0m ABCB1 Human ELISA, WB, IF, FC
DARC Recombinant Monoclonal Antibody CSB-RA287686A0HU ACKR1 Human ELISA, FC
ACLY Monoclonal Antibody CSB-MA177768 ACLY Human,Mouse,Rat,Pig ELISA,WB,ICC,FC
ACLY Recombinant Monoclonal Antibody CSB-RA712206A0HU ACLY Human ELISA, WB, IF, FC
ACO1 Recombinant Monoclonal Antibody CSB-RA298469A0HU ACO1 Human ELISA, WB, IF, FC
ACTA1 Recombinant Monoclonal Antibody CSB-RA289956A0HU ACTA1 Human ELISA, IHC, FC
ACTA1 Recombinant Monoclonal Antibody CSB-RA001205A0HU ACTA1 Human, Mouse, Rat ELISA, WB, IHC, IF, FC
ACTB Monoclonal Antibody CSB-MA000091M1m ACTB Human, Mouse, Rat, Rabbit ELISA, WB, IHC, IF, FC
ADAM10 Recombinant Monoclonal Antibody CSB-RA001273MA1HU ADAM10 Human ELISA, FC
AGO2 Recombinant Monoclonal Antibody CSB-RA965823A0HU AGO2 Human ELISA, IF, FC
AKR1C3 Recombinant Monoclonal Antibody CSB-RA825204A0HU AKR1C3 Human ELISA, IF, FC
AKT1/2/3 Recombinant Monoclonal Antibody CSB-RA272392A0HU AKT1/2/3 Human ELISA, WB, IHC, FC
ALB Recombinant Monoclonal Antibody CSB-RA264109A0HU ALB Human ELISA, FC
ALPP Recombinant Monoclonal Antibody CSB-RA183247A0HU ALPP Human ELISA, WB, IHC, FC
ANPEP Recombinant Monoclonal Antibody CSB-RA001827MA1HU ANPEP Human ELISA, IF, FC
ANXA1 Recombinant Monoclonal Antibody CSB-RA001836MA1HU ANXA1 Human ELISA, IHC, FC
ANXA4 Recombinant Monoclonal Antibody CSB-RA001845MA1HU ANXA4 Human, Mouse ELISA, WB, FC
APOA2 Recombinant Monoclonal Antibody CSB-RA001915A0HU APOA2 Human ELISA, WB, IHC, FC
APOL1 Recombinant Monoclonal Antibody CSB-RA966671A0HU APOL1 Human ELISA, FC
ARG2 Recombinant Monoclonal Antibody CSB-RA216908A0HU ARG2 Human ELISA, IHC, IF, FC

Isotype Control Antibodies:

Product name Code Fluorescent labeling Application
IgG1 Monoclonal Antibody CSB-MA255382 FITC FC
IgG1 Monoclonal Antibody CSB-MA792617 PE FC
IgG2a Monoclonal Antibody CSB-MA081360 FITC ELISA,IF,FC
IgG2a Monoclonal Antibody CSB-MA072925 PE ELISA,IF,FC
IgG2b Monoclonal Antibody CSB-MA993519 FITC ELISA,IF,FC
IgG2b Monoclonal Antibody CSB-MA982879 PE ELISA,IF,FC

Fluorescent Secondary Antibodies:

Product name Code Fluorescent labeling Application
Goat Anti-Mouse IgG(H+L) Antibody CSB-PA980279 FITC IHC,IF,FC
Goat Anti-Rabbit IgG(H+L) Antibody CSB-PA340892 FITC IHC,IF,FC
Goat Anti-Human IgG, Fcγ fragment specific CSB-PA271513 FITC FC


Q: Why is high background in flow cytometry (FC)?

a. Too high gain set. You should reduce the gain to decrease the signal and use the positive control to set up the flow cytometer correctly again.

b. Too much antibody. You should decrease the antibody concentration.

Q: Why are there two or more cell populations observed in flow cytometry (FC)?

a. More than one cell population expressing target protein. You should check if there is adequate cell separation.

b. Cell doublets present. Doublets of cells will show as a second cell population at approximately twice the fluorescence intensity on the plot. You should mix cells gently using a pipette before staining and before running on the cytometer.

Q: How should antibodies be selected in double-labeled immunofluorescence?

When performing double-labeling immunofluorescence experiments, the selection of appropriate antibodies is crucial for observing the co-localization of two antigenic proteins in cells. Below are the antibody selection options for different situations:

a. Two directly labeled antibodies: If available, two directly labeled primary antibodies with different fluorescent markers should be selected. This reduces the risk of cross-reactivity of the secondary antibodies and is easier to handle.

b. Two indirectly labeled antibodies: In this case, two primary antibodies with different host sources (Host), each paired with a different secondary antibody with different fluorescent markers, need to be selected to avoid cross-reactivity.

c. One direct-labeled antibody plus one indirectly labeled antibody: If one is from a direct-labeled primary antibody and the other is unlabeled, then a primary antibody of a different host source and the corresponding secondary antibody can be selected. Both antibodies can be incubated at the same time because the directly labeled primary antibody will not cross-react with the secondary antibody of the other primary antibody.

d. Two indirectly labeled antibodies of the same host origin: If the two primary antibodies are of the same host origin, it is necessary to incubate one primary antibody and use the corresponding secondary antibody for detection, then incubate the second primary antibody and use its corresponding secondary antibody. This avoids cross-reactivity between secondary antibodies of the same host source.

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1