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Rat Protein S100-A1(S100A1) ELISA kit

Citations (4) Protocol MSDS
Code CSB-EL020622RA
Size 96T,5×96T,10×96T
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Product Details

Target Name
S100 calcium binding protein A1
Alternative Names
S100a1 ELISA Kit; Protein S100-A1 ELISA Kit; S-100 protein alpha chain ELISA Kit; S-100 protein subunit alpha ELISA Kit; S100 calcium-binding protein A1 ELISA Kit
Abbreviation
S100A1
Uniprot No.
P35467
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 ng/mL-400 ng/mL
Sensitivity
1.56 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat S100A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 93
Range % 89-97
1:2 Average % 87
Range % 82-91
1:4 Average % 98
Range % 94-102
1:8 Average % 101
Range % 95-107
Recovery
The recovery of rat S100A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 84 80-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
400 2.043 2.001 2.022 1.851
200 1.295 1.327 1.311 1.140
100 0.888 0.852 0.870 0.699
50 0.608 0.640 0.624 0.453
25 0.430 0.460 0.445 0.274
12.5 0.346 0.329 0.338 0.167
6.25 0.255 0.238 0.247 0.076
0 0.168 0.174 0.171 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat S100A1 ELISA Kit was designed for the quantitative measurement of Rat S100A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

Citations

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Target Background

Function
(From Uniprot)
Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation. In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes. These changes allow interactions with specific target proteins and modulate their activity. Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase. Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole.
Gene References into Functions
  1. depletion of S100A1 was observed in ischemic cardiomyocytes, and S100A1 plasma concentration was also significantly higher than that of the sham-operated group PMID: 23683996
  2. S100A1 is involved in the maintenance of the genetic program that defines normal myocardial function and its downregulation is permissive for the induction of genes that underlie myocardial hypertrophy PMID: 12619862
  3. S100A1 and calmodulin compete for the same binding site on ryanodine receptor PMID: 18650434
  4. in sympathetic neurons exogenous S100A1 may lead to an increase of sympathetic output. PMID: 19657060
Subcellular Location
Cytoplasm. Sarcoplasmic reticulum. Mitochondrion.
Protein Families
S-100 family
Tissue Specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
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