JAR1 Antibody

Code CSB-PA879774XA01DOA
Size Enquire
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) JAR1 Polyclonal antibody
Uniprot No. Q9SKE2
Target Names JAR1
Alternative Names JAR1 antibody; FIN219 antibody; At2g46370 antibody; F11C10.6 antibody; Jasmonoyl--L-amino acid synthetase JAR1 antibody; EC 6.3.2.52 antibody; Jasmonate-amino acid synthetase JAR1 antibody; Jasmonic acid-amido synthetase JAR1 antibody; Protein FAR-RED INSENSITIVE 219 antibody; Protein JASMONATE RESISTANT 1 antibody
Raised in Rabbit
Species Reactivity Arabidopsis thaliana
Immunogen Recombinant Arabidopsis thaliana JAR1 protein
Immunogen Species Arabidopsis thaliana (Mouse-ear cress)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Catalyzes the synthesis of jasmonates-amino acid conjugates by adenylation; can use Ile and, in vitro at least, Val, Leu and Phe as conjugating amino acids on jasmonic acid (JA) and 9,10-dihydro-JA substrates, and to a lower extent, on 3-oxo-2-(2Z-pentenyl)-cyclopentane-1-butyric acid (OPC-4) and 12-hydroxy-JA (12-OH-JA). Can synthesize adenosine 5-tetraphosphate in vitro. Required for the JA-mediated signaling pathway that regulates many developmental and defense mechanisms, including growth root inhibition, vegetative storage proteins (VSPs) accumulation, induced systemic resistance (ISR), response to wounding and herbivores, tolerance to ozone O(3) (probably having a role in lesion containment). Plays an important role in the accumulation of JA-Ile in response to wounding, both locally and systemically; promotes JA responding genes especially in distal part of wounded plants, via the JA-Ile-stimulated degradation of JAZ repressor proteins by the SCF(COI)E3 ubiquitin-protein ligase pathway. Involved in the apoptosis-like programmed cell death (PCD) induced by fungal toxin fumonisin B1-mediated (FB1). Required for volatile compounds (C6-aldehydes and allo-ocimene)-mediated defense activation. Involved in the non-pathogenic rhizobacterium-mediated ISR (defense priming) by P.fluorescens (strains CHAOr and WCS417r) and P.putida LSW17S against infection leaf pathogens such as P.syringae pv. tomato and H.parasitica. Required for the JA-dependent resistance to fungi such as P.irregulare, U.vignae and U.appendiculatus. Necessary to induce systemic resistance against R.solanaceraum and P.syringae pv. tomato with P.oligandrum (a non-pathogenic biocontrol agent) cell wall protein fraction (CWP). Mediates PGIP2 accumulation in response to B.cinerea infection and thus contributes to resistance against this pathogen. Modulates the UV-B alteration of leaves attractiveness to diamondback moths P.xylostella leading to insect oviposition. Involved in the regulation of far-red light influence on development, being an actor of the interplay between light and JA signaling. Seems necessary for the salicylic acid (SA)-mediated, NPR1-independent resistance pathway. May contribute to the chitin-elicited pathway. Contributes to the sensitivity toward F.graminearum.
Gene References into Functions
  1. FIN219/JAR1 and CRY1 antagonize each other to modulate photomorphogenic development of seedlings and stress responses in Arabidopsis.FIN219 and CRY1 negatively regulated each other by direct interaction in response to jasmonate under blue light. PMID: 29561841
  2. We show that the rotated C-terminal domain of FIN219 alters ATP binding and the core structure of the active site. We further demonstrate that this unique FIN219-FIP1 structure is crucial for increasing FIN219 activity and determines the priority of substrate binding. PMID: 28223489
  3. FIN219/JAR1 is tightly regulated in response to exogenous jasmonic acid. PMID: 26176841
  4. crystal structures of benzoate-specific Arabidopsis thaliana AtGH3.12/PBS3 and jasmonic acid-specific AtGH3.11/JAR1 PMID: 22628555
  5. FIN219 interacts with FIP1 to regulate cell elongation and flowering in response to light. PMID: 17220357
  6. analyzed the substrate specificity of recombinant JAR1 and determined whether it catalyzes the synthesis of mono- and dinucleoside polyphosphates PMID: 17291501
  7. Increase in jasmonic acid-isoleucine mediated by the JAR1 enzyme plays only a minor role in transcriptional modulation of genes induced by mechanical wounding. PMID: 18247047

Show More

Hide All

Subcellular Location Cytoplasm.
Protein Families IAA-amido conjugating enzyme family
Database Links

KEGG: ath:AT2G46370

STRING: 3702.AT2G46370.4

UniGene: At.13902

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2023 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1