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Code | CSB-PA879774XA01DOA |
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Full Product Name | Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) JAR1 Polyclonal antibody |
Uniprot No. | Q9SKE2 |
Target Names | JAR1 |
Alternative Names | JAR1 antibody; FIN219 antibody; At2g46370 antibody; F11C10.6 antibody; Jasmonoyl--L-amino acid synthetase JAR1 antibody; EC 6.3.2.52 antibody; Jasmonate-amino acid synthetase JAR1 antibody; Jasmonic acid-amido synthetase JAR1 antibody; Protein FAR-RED INSENSITIVE 219 antibody; Protein JASMONATE RESISTANT 1 antibody |
Raised in | Rabbit |
Species Reactivity | Arabidopsis thaliana |
Immunogen | Recombinant Arabidopsis thaliana JAR1 protein |
Immunogen Species | Arabidopsis thaliana (Mouse-ear cress) |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | Protein A/G |
Concentration | It differs from different batches. Please contact us to confirm it. |
Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Tested Applications | ELISA, WB (ensure identification of antigen) |
Protocols | ELISA Protocol |
Troubleshooting and FAQs | Antibody FAQs |
Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Value-added Deliverables | ① 200ug * antigen (positive control); ② 1ml * Pre-immune serum (negative control); |
Quality Guarantee | ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection; ② ELISA titer can be guaranteed 1: 64,000; ③ WB validation with antigen can be guaranteed positive; |
Lead Time | Made-to-order (14-16 weeks) |
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Function (From Uniprot) |
Catalyzes the synthesis of jasmonates-amino acid conjugates by adenylation; can use Ile and, in vitro at least, Val, Leu and Phe as conjugating amino acids on jasmonic acid (JA) and 9,10-dihydro-JA substrates, and to a lower extent, on 3-oxo-2-(2Z-pentenyl)-cyclopentane-1-butyric acid (OPC-4) and 12-hydroxy-JA (12-OH-JA). Can synthesize adenosine 5-tetraphosphate in vitro. Required for the JA-mediated signaling pathway that regulates many developmental and defense mechanisms, including growth root inhibition, vegetative storage proteins (VSPs) accumulation, induced systemic resistance (ISR), response to wounding and herbivores, tolerance to ozone O(3) (probably having a role in lesion containment). Plays an important role in the accumulation of JA-Ile in response to wounding, both locally and systemically; promotes JA responding genes especially in distal part of wounded plants, via the JA-Ile-stimulated degradation of JAZ repressor proteins by the SCF(COI)E3 ubiquitin-protein ligase pathway. Involved in the apoptosis-like programmed cell death (PCD) induced by fungal toxin fumonisin B1-mediated (FB1). Required for volatile compounds (C6-aldehydes and allo-ocimene)-mediated defense activation. Involved in the non-pathogenic rhizobacterium-mediated ISR (defense priming) by P.fluorescens (strains CHAOr and WCS417r) and P.putida LSW17S against infection leaf pathogens such as P.syringae pv. tomato and H.parasitica. Required for the JA-dependent resistance to fungi such as P.irregulare, U.vignae and U.appendiculatus. Necessary to induce systemic resistance against R.solanaceraum and P.syringae pv. tomato with P.oligandrum (a non-pathogenic biocontrol agent) cell wall protein fraction (CWP). Mediates PGIP2 accumulation in response to B.cinerea infection and thus contributes to resistance against this pathogen. Modulates the UV-B alteration of leaves attractiveness to diamondback moths P.xylostella leading to insect oviposition. Involved in the regulation of far-red light influence on development, being an actor of the interplay between light and JA signaling. Seems necessary for the salicylic acid (SA)-mediated, NPR1-independent resistance pathway. May contribute to the chitin-elicited pathway. Contributes to the sensitivity toward F.graminearum. |
Gene References into Functions |
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Subcellular Location | Cytoplasm. |
Protein Families | IAA-amido conjugating enzyme family |
Database Links |
KEGG: ath:AT2G46370 STRING: 3702.AT2G46370.4 UniGene: At.13902 |