MUS81 Antibody

Code CSB-PA449502XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MUS81 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
MUS81 antibody; SLX3 antibody; YDR386W antibody; D9509.6 antibody; Crossover junction endonuclease MUS81 antibody; EC 3.1.22.- antibody; MMS and UV-sensitive protein 81 antibody
Raised in
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MUS81 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Purification Method
Protein A/G
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA, WB (ensure identification of antigen)
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Interacts with MMS4 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks with regressed leading strands and nicked Holliday junctions. Cleavage probably occurs approximately half a helical turn upstream of the free 5'-end in these structures. May be required in mitosis for the processing of stalled replication fork intermediates arising spontaneously or subsequent to treatment with DNA damaging agents such as methylmethane sulfonate (MMS), camptothecin (CPT) or UV. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI). This involves consecutive cleavage of D-loops and nicked Holliday junctions leading to sister chromatid crossover. In contrast to MSH4-MSH5 dependent crossover, double Holliday junctions do not seem to be involved. Spore formation and viability are severely impaired in deletion strains.
Gene References into Functions
  1. Identification of new interacting partners and modulators of Mus81-Mms4 nuclease, RFC, and PCNA imply the cooperation of these factors in resolution of stalled replication forks and branched DNA structures emanating from the restarted replication forks under conditions of replication stress. PMID: 28969641
  2. The data suggest that subnuclear relocalization is relevant for the function of Mus81-Mms4 complex in response to DNA damage and, probably, of the endonucleases that colocalize with it. PMID: 28813668
  3. Our data suggest that Srs2 and Mus81-Mms4 have critical roles in preventing the formation of (or in resolving) toxic inter-homolog joint molecules, which could otherwise interfere with chromosome segregation and lead to genetic instability. PMID: 27390022
  4. The role of MUS81 in crossover formation during meiotic recombinational repair PMID: 25329811
  5. Study found that the resolvase Mus81p is required for most of the UV-induced inter-homolog recombination events; this requirement likely reflects the Mus81p-associated cleavage of dimer-blocked replication forks. PMID: 25738287
  6. Unresolved recombination intermediates are a source of mitotic sister chromatid bridges, and Mus81-Mms4 and Yen1 play a central role in their resolution in vivo. PMID: 25466415
  7. this study proposes that lack of a timely converging fork or Mus81 may propel genome instability observed in cancer. PMID: 26273056
  8. data point to a coordinated role of Mus81-Mms4 and Srs2 in processing of recombination as well as replication intermediates PMID: 25765656
  9. findings indicate that the N-terminal region of Mus81 acts as a landing pad to interact with Rad27 and that Mus81 and Rad27 work conjointly for efficient removal of various aberrant DNA structures PMID: 25628354
  10. The data indicates that Mus81-Mms4 activation is not induced by the presence of DNA lesions and that its function to respond to DNA damage, which allows cell survival, is carried out after completion of bulk genome replication. PMID: 23901010
  11. Suggest that Mph1 and Mus81-Mms4 recognize an early strand exchange intermediate and direct repair to noncrossover or crossover outcomes, respectively. PMID: 24119400
  12. Premature activation of the Cdk1/Cdc5/Mus81 pathway induces crossover-associated chromosome translocations and precocious processing of damage-bypass sister chromatid junction intermediates. PMID: 23531881
  13. data support a model in which Mus81-Mms4 cleaves nicked recombination intermediates such as displacement loops (D-loops), nicked Holliday junctions, or 3' flaps but not intact Holliday junctions with four uninterrupted strands. PMID: 22645308
  14. Using an assay to detect unselected products of mitotic recombination, we found a significant decrease in crossovers in the Saccharomyces cerevisiae mus81Delta mutant. PMID: 21172663
  15. Functional overlap between the structure-specific nucleases Yen1 and Mus81-Mms4 for DNA-damage repair in S. cerevisiae PMID: 20106725
  16. Yen1 has a role in the response to DNA damage; HJ resolution functions of Yen1 overlap with Mus81 during replication fork repair PMID: 20178992
  17. rnh202 cells display a sensitivity to DNA-damaging agents that is exacerbated in the absence of RAD51 or MUS81. PMID: 16193328
  18. The purification of active forms of recombinant Schizosaccharomyces pombe Mus81-Eme1 and Saccharomyces cerevisiae Mus81-Mms4, which display robust Holliday junction cleavage in vitro, is reported. PMID: 17363897
  19. A kinetic analysis of Mus81-Mms4 cleavage, and a quantitative comparison of its substrate selectivity. PMID: 18281703
  20. These results indicate that Sgs1 and Mus81/Mms4 collaborate to direct meiotic recombination toward interhomolog interactions that promote proper chromosome segregation, and also indicate that Mus81/Mms4 promotes joint molecules resolution in vivo. PMID: 18691964
  21. Sgs1 and Mus81-Mms4 collaborate to eliminate aberrant JMs, whereas as-yet-unidentified enzymes process normal joint molecules. PMID: 18691965
  22. In vivo cruciform resolution requires Mus81, for which the bacterial HJ resolvase RusA will substitute. PMID: 18922464
  23. Results indicate that Mus81-Mms4, unlike FEN1/Rad27, does not prefer dually flapped substrates and is unlikely to function as a 3'-flapase counterpart to the 5'-flapase activity of FEN1/Rad27. PMID: 19211663

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Subcellular Location
Protein Families
XPF family
Database Links

KEGG: sce:YDR386W

STRING: 4932.YDR386W

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