Human α2-Antiplasmin,α2-AP ELISA Kit

Code CSB-E09452h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Alternative Names
A2AP ELISA Kit; A2AP_HUMAN ELISA Kit; AAP ELISA Kit; Alpha 2 antiplasmin ELISA Kit; Alpha 2 antiplasmin pigment epithelium derived factor ELISA Kit; Alpha 2 AP ELISA Kit; ALPHA 2 PI ELISA Kit; Alpha 2 plasmin inhibitor ELISA Kit; Alpha 2 plasmin inhibitor deficiency ELISA Kit; Alpha-2-antiplasmin ELISA Kit; Alpha-2-AP ELISA Kit; Alpha-2-PI ELISA Kit; Alpha-2-plasmin inhibitor ELISA Kit; Antiplasmin deficiency ELISA Kit; API ELISA Kit; Plasmin inhibitor deficiency ELISA Kit; PLI ELISA Kit; Serine (or cysteine) peptidase inhibitor; clade F; member 2 ELISA Kit; Serine (Or cysteine) peptidase inhibitor; clade F; member 2; isoform CRA_c ELISA Kit; Serine (or cysteine) proteinase inhibitor; clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 ELISA Kit; Serine (Or cysteine) proteinase inhibitor; clade F; member 2 ELISA Kit; Serine or cysteine peptidase inhibitor clade F member 2 ELISA Kit; Serpin F2 ELISA Kit; Serpin peptidase inhibitor clade F ELISA Kit; Serpin peptidase inhibitor; clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 ELISA Kit; SERPINF2 ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
15.6 pg/mL-1000 pg/mL
3.9 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human α2-AP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %88
Range %84-93
1:2Average %85
Range %81-91
1:4Average %92
Range %88-100
1:8Average %95
Range %91-99
The recovery of human α2-AP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8983-94
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
10002.272 2.346 2.309 2.214
5001.476 1.491 1.484 1.389
2500.863 0.892 0.878 0.783
1250.448 0.479 0.464 0.369
62.50.268 0.251 0.260 0.165
31.20.187 0.178 0.183 0.088
15.60.169 0.159 0.164 0.069
00.097 0.092 0.095  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human SERPINF2 ELISA Kit was designed for the quantitative measurement of Human SERPINF2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

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Target Background

(From Uniprot)
Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
Gene References into Functions
  1. alpha2AP has a profibrotic effect probably not by the action as a plasmin inhibitor, and the blocking of alpha2AP exerts an antifibrotic effect in humans and mice with systemic sclerosis PMID: 26743600
  2. This review presents recent findings regarding the main aspects of the natural heterogeneity of A2AP with particular focus on the functional and possible clinical implications. [review] PMID: 26626994
  3. Possession of the alpha2AP 407Lys allele was negatively associated with AAA, and thus changes in alpha2AP may affect aneurysm growth and development. PMID: 25065555
  4. Changes in plasma A2AP are associated with cardiovascular disease event presentation. PMID: 26088309
  5. A significant correlation was found between soluble fibroblast activation protein levels and alpha2-antiplasmin cleavage in coronary thrombosis. PMID: 25464232
  6. increased N-terminal cleavage of alpha2AP may have a role in liver cirrhosis PMID: 24034420
  7. FXIII-A has a functional role through exposure on the activated platelet membrane where it exerts antifibrinolytic function by cross-linking alpha2AP to fibrin PMID: 25331118
  8. Data suggest serum A2AP (SERPINF2) level can serve as biomarker for diabetic retinopathy; levels of A2AP (but not fibrinogen, plasminogen, or plasminogen activator inhibitor 1) are up-regulated in hyperglycemic type 1 diabetes with retinopathy. PMID: 24818165
  9. protective mediator during gram-negative (pneumo)sepsis, limiting bacterial growth, inflammation, tissue injury, and coagulation PMID: 23992406
  10. Study revealed that plasmin was present in tumor tissue, and that it was responsible for processing progalanin to galanin(1-20) in the extracellular environment. PMID: 21707521
  11. Data suggest that decreased amounts of alpha2-plasmin inhibitor in plasma vs serum ex vivo may reflect reduced factor XIII (FXIII) in vivo; thus, plasma vs serum alpha2-plasmin inhibitor may be useful diagnostic marker for severe FXIII deficiency. PMID: 22205503
  12. fibrinolysis is enhanced by inhibiting enzymatic cleavage of precursor alpha2-antiplasmin PMID: 21251197
  13. Truncation of monocyte chemoattractant protein 1 by plasmin promotes blood-brain barrier disruption. PMID: 21486949
  14. Levels of free full-length alpha2AP were decreased in myocardial infarction (MI); the percentage of C-terminally cleaved alpha2AP was unaltered, and Arg407Lys did not influence alpha2AP levels or MI risk. PMID: 21505192
  15. the antifibrinolytic function of FXIII is independent of fibrin-fibrin cross-linking and is expressed exclusively through alphaAP. PMID: 21471521
  16. ADAMTS13 inactivation by plasmin as a potential cause of thrombotic thrombocytopenic purpura PMID: 20553378
  17. deficiency in patients with Philadelphia chromosome-positive haematologic cancer receiving imatinib mesylate PMID: 19492163
  18. OPN is highly susceptible to cleavage near its integrin-binding motifs, and the protein is a novel substrate for plasmin and cathepsin D PMID: 20071328
  19. FXIIIa substrate, A2AP, sequences suggests that the residue located two positions beyond the reactive glutamine is not conserved. this position makes important contributions to effective FXIIIa-A2AP interactions PMID: 19691486
  20. Multiple Lys residues within alpha 2-antiplasmin contribute, perhaps in a zipper-like fashion, to its binding to the in-tandem, multikringle array that configures the plasmin heavy chain. PMID: 12549929
  21. Alpha2-antiplasmin has an important role in acute pulmonary embolism PMID: 12911586
  22. the Arg6Trp polymorphism may play a significant role in governing the long-term deposition/removal of intravascular fibrin PMID: 17317851
  23. Fibrinolysis is amplified by converting alpha-antiplasmin from a plasmin inhibitor to a substrate PMID: 17883703
  24. Hydroxyethyl starch (HES) dilution enhances fibrinolysis by diminishing alpha2-antiplasmin-plasmin interactions. PMID: 17890952
  25. Sequencing analysis revealed the presence of two alpha2-PI gene variations, both in the second half of exon 10: a frameshift mutation and a G to A transition at nucleotide 11337 in codon 407. PMID: 17961166
  26. Thrombin activatable fibrinolysis inhibitor and alpha(2)-antiplasmin are not markers for recanalization in patients with ischemic stroke treated with recombinant tissue-type plasminogen activator. PMID: 18048863
  27. Plasmin in nephrotic urine activates the epithelial sodium channel PMID: 19073825

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Involvement in disease
Alpha-2-plasmin inhibitor deficiency (APLID)
Subcellular Location
Protein Families
Serpin family
Tissue Specificity
Expressed by the liver and secreted in plasma.
Database Links

HGNC: 9075

OMIM: 262850

KEGG: hsa:5345

STRING: 9606.ENSP00000321853

UniGene: Hs.159509

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