Human Actin, cytoplasmic 2(ACTG1) ELISA kit

Instructions
Code CSB-EL001222HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name actin, gamma 1
Alternative Names ACT ELISA Kit; ACTB ELISA Kit; ACTG ELISA Kit; ACTG_HUMAN ELISA Kit; actg1 ELISA Kit; Actin ELISA Kit; Actin; cytoplasmic 2 ELISA Kit; Actin; gamma 1 ELISA Kit; Actin; gamma 1 propeptide ELISA Kit; Actin; gamma ELISA Kit; BRWS2 ELISA Kit; cytoplasmic 2 ELISA Kit; Cytoskeletal gamma actin ELISA Kit; Deafness; autosomal dominant 20 ELISA Kit; Deafness; autosomal dominant 26 ELISA Kit; DFNA20 ELISA Kit; DFNA26 ELISA Kit; epididymis luminal protein 176 ELISA Kit; Gamma-actin ELISA Kit; HEL-176 ELISA Kit; N-terminally processed ELISA Kit
Abbreviation ACTG1
Uniprot No. P63261
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ACTG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 89-99  
1:2 Average % 105  
Range % 101-108  
1:4 Average % 94  
Range % 90-98  
1:8 Average % 98  
Range % 94-102  
Recovery
The recovery of human ACTG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-98  
EDTA plasma (n=4) 86 80-91  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1600 2.143 2.284 2.214 2.074  
800 1.441 1.522 1.482 1.342  
400 0.832 0.807 0.820 0.680  
200 0.493 0.506 0.500 0.360  
100 0.301 0.317 0.309 0.169  
50 0.237 0.245 0.241 0.101  
25 0.189 0.183 0.186 0.046  
0 0.139 0.140 0.140    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

Function
(From Uniprot)
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Gene References into Functions
  1. A novel actin gamma 1 (ACTG1) de novo mutation is identified in two sporadic, juvenile, Chinese non-syndromic hearing loss (NSHL) cases using targeted high-throughput sequencing. PMID: 29357087
  2. Results identified ACTG1 with significant high expression in skin cancer tissue and suggested that ACTG1 can regulate the cell proliferation and migration through ROCK signaling pathway. PMID: 28727228
  3. a novel heterozygous missense mutation P32S in the ACTG1 gene was identified in a small family with autosomal dominant nonsyndromic hearing loss. The present findings expand our understanding of the phenotypes associated with ACTG1. Specifically, the results of the present study emphasized that mutations in ACTG1 result in a diverse spectrum of onset ages, including congenital in addition to post-lingual onset. PMID: 29620237
  4. Data reports a recurrent de novo mutation in ACTG1 causes isolated ocular coloboma PMID: 28493397
  5. We have identified a three-generation pedigree segregating a novel mutation in the ACTG1 gene that causes Baraitser-Winter Syndrome with extremely variable expressivity, leading to an initial diagnosis of isolated AD hearing loss in two members. PMID: 27096712
  6. we expand the clinical spectrum of the ACTG1-associated Baraitser-Winter cerebrofrontofacial syndrome demonstrating the mild end of the facial and brain manifestations. PMID: 27240540
  7. Data indicate that F-actin is significantly elevated in septic shock, and F-actin and the F:G-actin ratio are potential biomarkers for the diagnosis of septic shock. PMID: 26754286
  8. a novel mutation in ACTG1 was found to be co-segregated with hearing loss and the genetic cause of autosomal dominant nonsyndromic hearing impairment in a Chinese family PMID: 26832775
  9. Three unrelated cases of rare ACTG1 variants in fetal microlissencephaly have been described. PMID: 26188271
  10. In this study we sought to clarify the detailed mutational spectrum, clinical features, and genotype-phenotype correlations for ACTG1 gene associated autosomal dominant sensorineural hearing loss PMID: 25792668
  11. The data, for the first time, link ASAP3 with ACTG1 in the regulation of cytoskeletal maintenance and cell motility PMID: 24284654
  12. The actin/MKL1 signalling pathway influences cell growth and gene expression through large-scale chromatin reorganization and histone post-translational modifications. PMID: 24762104
  13. the novel variant p.M305T in ACTG1 (DFNA20/26) was selected as a disease-causing variant. PMID: 23506231
  14. These results showed the biphasic F-actin dynamics in herpes simplex virus 1 neuronal infection and confirmed the association of F-actin with the changes in the expression and activity of cofilin 1. PMID: 22623803
  15. knockdown of gamma-actin significantly reduced speed of motility and severely affected the cell's ability to explore, which was, in part, due to a loss of cell polarity PMID: 21908715
  16. Cytoplasmic G-actin concentration is a critical parameter for determining the extent of stimulus-induced G-actin assembly and cell extension. PMID: 21502360
  17. actin participates in transcription elongation by recruiting Cdk9,a catalytic subunit of P-TEFb, for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling PMID: 21378166
  18. RNA polymerase II accumulates in the promoter-proximal region of the dihydrofolate reductase and gamma-actin genes. PMID: 12612070
  19. the first description of a mutation in cytoskeletal, or nonmuscle, actin; with an autosomal dominant, progressive, sensorineural hearing loss phenotype PMID: 13680526
  20. A missense mutation in the gamma actin 1 gene causes autosomal dominant hearing loss. PMID: 14684684
  21. Our data are consistent with the idea that DP assembles into nascent junctions from both diffusible and particulate pools in a temporally overlapping series of events triggered by cell-cell contact and regulated by actin and DP-IF interactions. PMID: 16365169
  22. Espins do not activate the Arp2/3 complex in vitro, and bundle assembly is not indicative of in-vitro nucleation activity.Our results suggest a novel way to build actin bundles at specific sites in cells. PMID: 16569662
  23. A major factor in the deafness caused by gamma-actin mutations is an altered ability of the actin filaments to be properly regulated by actin-binding proteins rather than an inability to polymerize. PMID: 16690605
  24. the present results do not indicate that mutations in ACTG1 are a frequent cause of autosomal-dominant postlingual sensorineural hearing impairment in Norway nor Denmark. PMID: 16773128
  25. actin and vimentin filaments can interact directly through the tail domain of vimentin PMID: 16901892
  26. During cell membrane blebbing actin recruitment is involved in cortex assembly. PMID: 17088428
  27. In both AA and AL renal amyloidosis groups there were significant positive correlations between immunostaining of TGFbeta-1 and alpha-SMA. PMID: 17285762
  28. IQGAP1 regulates Salmonella invasion through interactions with actin, Rac1, and Cdc42 PMID: 17693642
  29. In this report we have used mutagenesis of the Talin1 I/LWEQ module to show that the amino acids responsible for dimerization are necessary for F-actin binding, stabilization and cross-linking of actin filaments, and focal adhesion targeting. PMID: 17722883
  30. analysis of the structural basis of profilin-actin complexes during filament elongation by Ena/VASP PMID: 17914456
  31. In this study, a novel missense mutation (c.364A>G; p.I122V) co-segregated with the affected individuals in the family and did not exist in the unaffected family members and 150 unrelated normal controls. PMID: 18804074
  32. Both RPEL peptides of the MAL protein bind to the G-actin hydrophobic cleft and to subdomain 3. PMID: 19008859
  33. Two novel ACTG1 missense mutations are associated with DFNA20/26 hearing impairment. PMID: 19477959
  34. audiometric phenotype of the Dutch DFNA20/26 family with a novel mutation in ACTG1 was largely consistent with previous reports on DFNA20/26. All suffered from hearing loss.This is the first known DFNA20/26 family that has experienced tinnitus. PMID: 19548389

Show More

Hide All

Involvement in disease Deafness, autosomal dominant, 20 (DFNA20); Baraitser-Winter syndrome 2 (BRWS2)
Subcellular Location Cytoplasm, cytoskeleton
Protein Families Actin family
Database Links

HGNC: 144

OMIM: 102560

KEGG: hsa:71

STRING: 9606.ENSP00000331514

UniGene: Hs.514581

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1