Human Agouti Related Protein,AGRP ELISA Kit

Instructions
Code CSB-E09299h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name agouti related protein homolog (mouse)
Alternative Names AGRT, ART, ASIP2, MGC118963, agouti related protein
Abbreviation AGRP
Uniprot No. O00253
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, urine
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 0.002 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

 

Three samples of known concentration were tested twenty times on one plate to assess.

 

Inter-assay Precision (Precision between assays): CV%<10%

 

Three samples of known concentration were tested in twenty assays to assess.

  

Linearity

 To assess the linearity of the assay, samples were spiked with high concentrations of human AGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (By similarity). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.
Involvement in disease Obesity (OBESITY)
Subcellular Location Secreted, Golgi apparatus lumen
Tissue Specificity Expressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.
Database Links

HGNC: 330

OMIM: 601665

KEGG: hsa:181

STRING: 9606.ENSP00000290953

UniGene: Hs.104633

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