Human Carbonyl reductase [NADPH] 1 (CBR1/CBR/CRN) ELISA kit

Instructions
Code CSB-E17009h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name carbonyl reductase 1
Alternative Names 15 hydroxyprostaglandin dehydrogenase [NADP+] ELISA Kit; 15-hydroxyprostaglandin dehydrogenase [NADP+] ELISA Kit; Carbonyl reductase [NADPH] 1 ELISA Kit; Carbonyl Reductase 1 ELISA Kit; CBR 1 ELISA Kit; CBR1 ELISA Kit; CBR1_HUMAN ELISA Kit; CRN ELISA Kit; NADPH dependent carbonyl reductase 1 ELISA Kit; NADPH-dependent carbonyl reductase 1 ELISA Kit; Prostaglandin 9 ketoreductase ELISA Kit; Prostaglandin 9-ketoreductase ELISA Kit; Prostaglandin E(2) 9 reductase ELISA Kit; Prostaglandin-E(2) 9-reductase ELISA Kit; SDR21C1 ELISA Kit
Abbreviation CBR1
Uniprot No. P16152
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CBR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-97
1:2 Average % 85
Range % 80-89
1:4 Average % 94
Range % 90-98
1:8 Average % 102
Range % 98-107
Recovery
The recovery of human CBR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 92-100
EDTA plasma (n=4) 89 84-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.319 2.214 2.267 2.142
1000 1.394 1.452 1.423 1.298
500 0.825 0.870 0.848 0.723
250 0.471 0.487 0.479 0.354
125 0.308 0.295 0.302 0.177
62.5 0.247 0.239 0.243 0.118
31.25 0.153 0.161 0.157 0.032
0 0.122 0.128 0.125  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

is it possible to use Cat#E17009H Human Carbonyl reductase [NADPH] 1 (CBR1/CBR/CRN) ELISA kit 96 tests for mouse samples too, is it possible to test this maybe?

A:
Thanks for your inquiry!
It is not recommended to use the kit CSB-E17009H to detect mouse samples.
Because the protein of mouse and human Carbonyl reductase CBR1 are different. Also the antibody specificity of 2 kits is different.
There exsits sample matrix difference between mouse and samples.
We advise you not use the kit of across species.

Target Data

Function NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
Gene References into Functions
  1. This study examined the impact of the functional single nucleotide polymorphism CBR1 rs9024 on the bioactivation of loxoprofen in a collection of human liver samples and tumor cell lines. PMID: 29851133
  2. CRB1 is an efficient catalyst for the reduction of glutathionylated aldehydes derived from lipid peroxidation. PMID: 27562619
  3. The ability of human carbonyl reductase 1 to efficiently catalyze the reduction of glutathionylated aldehydes derived from lipid peroxidation, that in the case of glutathionylated-4-hydroxyalkanals is associated to the ability to oxidize the hemiacetal hydroxyl group PMID: 28274719
  4. Data suggest that fatty acids and acyl-CoAs bind competitively with respect to substrate binding to carbonyl reductase 1 (CBR1), an enzyme involved in first-pass drug metabolism in intestinal mucosa; inhibition of CBR1 by these products of digestion may lead to food-drug interactions. PMID: 28450226
  5. AKR1C3 is the primary enzyme and CBR1 is a minor enzyme responsible for warfarin reduction in human liver cytosol. PMID: 27055738
  6. These results suggest that CR1 induces apoptosis by activating the caspase pathway via binding to TNFR1. PMID: 26499922
  7. Results demonstrate a trend toward decreased functional expression of selective hepatic reductases in ESRD livers. PMID: 26282591
  8. Critical insights into the substrate selectivity of hCBR1 and the interaction between hCBR1 and glutathione. PMID: 26381805
  9. Up-Regulation of Carbonyl Reductase 1 Renders Development of Doxorubicin Resistance in Human Gastrointestinal Cancers PMID: 26328486
  10. CBR1 decreases promoted tumor proliferation and growth as well as invasion and metastasis; CBR1 has potential to become a new candidate for molecular targeting therapy. PMID: 25572536
  11. Inhibition of CBR1 may increase the efficacy of daunorubicin in cancer tissue. PMID: 25541467
  12. Protein products of AKR1C1, AKR1C2, AKR7A3, CYP3A4, and carbonyl reductase (CBR1) were found in tumors and those of AKR1C1, AKR7A3, and CBR1 correlated with their transcript levels. PMID: 25526449
  13. we suggest that PEP-1-CBR1 protein may be a therapeutic agent for the treatment of ischemic injuries as well as oxidative-stress-induced cell damage and death. PMID: 24440593
  14. The stimulatory effect of cortisol on CBR1 expression may partly explain the concurrent increases of cortisol and prostaglandin PGF2alpha in human amnion tissue prior to the onset of labor PMID: 24654784
  15. Nrf2 is a novel transcriptional regulator of CBR1 genes in humans. PMID: 23247010
  16. GSNO-induced covalent modification of cysteine residues affects the kinetic mechanism of CBR1. PMID: 23295225
  17. regulation of human CBR1 expression by hsa-miR-574-5p and hsa-miR-921 depends upon rs9024 genotype status PMID: 23133646
  18. This pilot study suggests that CBR1 RNA expression may be helpful in identifying AML patients at risk of developing resistance or toxicity to daunorubicin due to increased formation of daunorubicinol. PMID: 22562609
  19. CBR1 regulates cancer cell invasion in endometrial adenocarcinomas by regulating the epithelial mesenchymal transition PMID: 22542806
  20. Polymorphisms in CBR1 gene did not increase risk of cardiomyopathy after anthracycline treatment of childhood cancers. PMID: 22124095
  21. a physiological role of CBR1, but not for CBR3, in S-nitrosoglutathione reduction and thus ultimately in regulation of NO signaling PMID: 21256830
  22. This protein has been found differentially expressed in thalami from patients with schizophrenia. PMID: 20471030
  23. analysis of the structural basis for substrate specificity in human monomeric carbonyl reductases CBR1 PMID: 19841672
  24. the functional genetic determinant of CBR1 activity toward relevant physiological and pharmacological substrates PMID: 17344335
  25. The functional characterization of the promoter of CBR1 is reported. PMID: 17569794
  26. Carbonyl reductase-1 (CBR1), microsomal prostaglandin E synthase-1 and 2 (mPGES-1, mPGES-2), cytosolic prostaglandin E synthase (cPGES), aldoketoreductase (AKR1C1) and prostaglandin F synthase (AKR1C3) were all expressed in hair follicles. PMID: 17697149
  27. These results suggested that hCBR3 and hCBR1 play distinct physiological roles. PMID: 18493841
  28. Human carbonyl reductase 1 is an S-nitrosoglutathione reductase PMID: 18826943
  29. CBR1 polymorphisms have a significant influence on the pharmacokinetics of doxorubicin in Asian breast cancer patients. PMID: 19016765
  30. the nonsynonymous single nucleotide polymorphisms generating mutations OF CBR1 may alter bioavailability of anthracyclines in cancer patients PMID: 19204081

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Subcellular Location Cytoplasm
Protein Families Short-chain dehydrogenases/reductases (SDR) family
Database Links

HGNC: 1548

OMIM: 114830

KEGG: hsa:873

STRING: 9606.ENSP00000290349

UniGene: Hs.88778

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