Human Chorionic Gonadotrophin,HCG ELISA Kit

Instructions
Code CSB-E05060h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Chorionic Gonadotrophin,HCG
Alternative Names CGB3 ELISA Kit; CGB; ELISA Kit; CGB5; ELISA Kit; CGB8 ELISA Kit; Choriogonadotropin subunit beta 3 ELISA Kit; Choriogonadotropin subunit beta ELISA Kit; CG-beta ELISA Kit; Chorionic gonadotropin chain beta ELISA Kit
Abbreviation hCG
Uniprot No. P0DN86
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, cell culture supernates
Detection Range 8 mIU/mL-240 mIU/mL
Sensitivity 2 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HCG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 86-97  
1:2 Average % 92  
Range % 85-99  
1:4 Average % 95  
Range % 92-100  
1:8 Average % 95  
Range % 89-98  
Recovery
The recovery of human HCG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 97 90-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average Corrected  
240 2.540 2.596 2.568 2.532  
100 1.279 1.299 1.289 1.253  
40 0.638 0.632 0.635 0.599  
16 0.285 0.291 0.288 0.252  
8 0.159 0.155 0.157 0.121  
0 0.036 0.035 0.036    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role in pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
Gene References into Functions
  1. risk for giving birth to a child with cerebral palsy was higher in women who did not have high hCG concentrations in the 1st trimester and low hCG concentrations in the 2nd trimester PMID: 30007247
  2. The presence of hCGbeta positive tumor cells appears to potentially reflect the aggressive behavior of mucoepidermoid carcinoma and adenoid cystic carcinoma PMID: 30197330
  3. Chorionic Gonadotropin, beta Subunit promoted the migration and invasion of CRC in vitro and in vivo but had no effect on the proliferation of tumor cells. PMID: 29357321
  4. the results of this study provide novel evidence supporting a role of hCG in regulating the differentiation of peripheral FOXP3(+) Tregs, which affect the pregnancy outcomes of patients with a history of recurrent implantation failure PMID: 28044377
  5. Serum hCG-beta levels of POD 12 and 14 and the fold increase of hCG-beta levels from POD 12 to 14 significantly predict pregnancy outcomes after fresh and frozen cycles. PMID: 27262839
  6. Data indicate the diagnostic criteria of chorionic gonadotropin beta subunit (beta-hCG) and alpha-Fetoprotein (alphaFP) in CSF and serum, which may advance the early and formal diagnosis of intracranial germ cell tumors (ICGCTs). PMID: 26968839
  7. All pregnancies with serious complications had serum beta-human chorionic gonadotropin levels of 1.0-5.0 mIU/ml. PMID: 26702770
  8. hCGB serum concentrations differed significantly between Asian and European women during the first trimester of pregnancy. PMID: 25858516
  9. reveals a positive feedback loop between glial cells missing 1 and Human chorionic gonadotropin regulating placental hCGbeta expression and cell differentiation PMID: 26503785
  10. For Down syndrome affected pregnancies, the median MoM of first trimester PAPP-A, PlGF, and AFP were significantly reduced, and free-beta-hCG were significantly elevated. PMID: 25846403
  11. First trimester hCGbeta was not associated with placenta accreta. PMID: 24226752
  12. Maternal serum hCGB was not associated with fetal open spina bifida. PMID: 24226839
  13. Data suggest that low serum levels of hCGB/ADAM12 are biomarkers in diagnosis of continued incomplete spontaneous abortion; here, hCGB/ADAM12 indicate success/failure of single dose of misoprostol/oxytocic in treatment of early pregnancy failure. PMID: 26051455
  14. Depending on its source of production, glycoforms of hCG display different biological activities and functions that are essential for pregnancy outcome.[review] PMID: 25707740
  15. CGB was higher in pregnant women with chronic renal insufficiency compared to controls and women with renal disease and normal renal function. PMID: 25395363
  16. Data suggest that initial serum beta-HCG levels in women after transfer of vitrified--warmed cleavage-stage embryos are lower than those after transfer of slow cooled--thawed cleavage-stage embryos. PMID: 25592975
  17. Median free betahCG MoM in the control group was 1.04, and 1.08 (P = 0.859) in the placenta praevia group compared with 0.81 in the abnormally invasive placentation group (P = 0.06). PMID: 25639820
  18. Case Report: Krukenberg tumor presenting as back pain and a positive urine pregnancy test due to elevated urinary CGB. PMID: 24708577
  19. Hyperglycosylated human chorionic gonadotropin is associated with gestational trophoblastic diseases PMID: 25275660
  20. Time-to-pregnancy >/=24 months in spontaneously conceived pregnancies is associated with decreased levels of PAPP-A and free beta-hCG. PMID: 24338604
  21. The concentration of cell-free beta-HCG mRNA in maternal plasma may be applicable to the prenatal diagnosis of placenta accreta. PMID: 25063251
  22. placenta-mediated adverse pregnancy outcome (PMAPO) before term but not PMAPO at term associated with elevated serum level in second trimester PMID: 25767942
  23. no association between prior pre-eclampsia and first trimester pregnancy level PMID: 24304164
  24. CGB - core fragment was isolated from the urine of women, pregnant with normal, molar and hyperemesis gravidarum pregnancies. Larger glycoforms were more abundant in the abnormal pregnancies. PMID: 24905406
  25. In pregnant women with inherited thrombophilia, PAPP-A levels were significantly higher and fbHCG levels were lower during the first trimester. PMID: 23795618
  26. During pregnancy, regulation of hCG beta subunit genes expression correlates with both methylation of their promoters and TFAP2A expression level. PMID: 25063728
  27. Majority of the patients in 15 cases of hCG positive CSs without typical syncytiotrophoblastic cells showed elevated level of human chorionic gonadotropin (hCG)in blood preoperatively. PMID: 24480432
  28. may directly stimulate angiogenesis PMID: 24560494
  29. Expression of beta-hCG may be seen in high-grade osteosarcoma, but frequent beta-hCG immunohistochemical expression by tumor cells, associated with clinically elevated serum beta-hCG, is rare. PMID: 24856811
  30. Unlike the bovine c-terminal peptide of HCG-beta that lacks O-glycans and determinants for circulatory survival, human HCG-beta has extended plasma half-life. PMID: 24239648
  31. results of the experiments showed that hCGbeta action leads to the increase in ERK and AKT kinases phosphorylation in cancer cells and indicate that these biological effects can be achieved independently of human chorionic gonadotropin presence PMID: 24554412
  32. CGB genes 1 and 2, previously considered as pseudogenes, are notably expressed by epithelial cancer cell lines. PMID: 24778026
  33. LC-MS/MS provided in-depth information on hCG variant composition of hCGn (99/642) and hCGbetan (99/692) and hCG variant specificity profiles and facilitated precise classification of the epitopes PMID: 24014048
  34. Hyperglycosylated CGB is superior to traditional hCG for predicting pregnancy outcome after in vitro fertilization. PMID: 24355054
  35. Initial human chorionic gonadotropin values did not predict treatment success in cases with complete hydatidiform mole. PMID: 24379071
  36. hCG isoforms have potential roles as biomarkers of embryo viability for embryo/blastocyst transfer in infertility treatment. PMID: 23439616
  37. Data suggest that hCGB is higher in vaginal washing fluid in cases and suspected cases of PPROM (preterm premature rupture of membranes) than in control subjects; thus, hCGB level in vaginal washing fluid may be diagnostic test/marker for PPROM. PMID: 23510392
  38. Data suggest that during first trimester, women with ectopic pregnancies have lower blood levels of CGB, progesterone, and estradiol and higher levels of luteinizing hormone and follicle stimulating hormone than normal pregnant women. PMID: 23148678
  39. Increases in early beta-chorionic gonadotropin levels are slower in vanishing twins than in singleton and twin pregnancies. PMID: 23541408
  40. Pulmonary adenocarcinoma and bone metastases diagnosed with paraneoplastic secretion of the beta subunit of human chorionic gonadotropin (beta-HCG). PMID: 23337393
  41. hCGbeta induced cell motility via promoting ERK1/2 phosphorylation and MMP-2 upregulation in human prostate cancer DU145 cells. PMID: 23424616
  42. The depth of trophoblastic tissue infiltration into tubal wall in ampullary pregnancies is correlated with serum beta-hCG levels, but not with gestational age. PMID: 23011731
  43. A complex neuroendocrine circuit exists in the retina, involving hCG secreting cells, hCG targets and hCG-release controlling cells. PMID: 23285091
  44. Human CG not only acts as a chemoattractant of regulatory T (Treg) cells but also plays a central role in pregnancy-induced immune tolerance. PMID: 23396945
  45. Elevation of serum human chorionic gonadotropin is associated with intratubular trophoblasts in the contralateral testis. PMID: 23136239
  46. we showed that type 1 and type 2 beta-hCG are highly expressed by villous mononucleated cytotrophoblasts in the early first trimester PMID: 22811468
  47. The serum levels of betahCG were significantly higher in pregnancies which subsequently developed preeclampsia. PMID: 22148978
  48. Ethnic group-specific median for free beta-hCG should be incorporated during first-trimester screening for fetal Down syndrome;The equation of best fit for free beta-hCG was: predicted median free beta-hCG level=465.332x10(-0.024xgestational age in days) PMID: 22365285
  49. Pregnancy-associated plasma protein A and free-beta-hCG values are gestational age specific. PMID: 22367671
  50. analysis of candidate genes revealed four genes that influence fertility: CGB/LHB gene cluster (p = 0.0036), FSHR (p = 0.023), FST (p = 0.023), and INHB; none of the independent SNPs in these genes predicted mortality PMID: 21222045
  51. Antibody recognition of a human chorionic gonadotropin epitope (hCGbeta66-80) depends on local structure retained in the free peptide. PMID: 21592960
  52. KLF6 playing a role as transcriptional regulator of relevant genes for placental differentiation and physiology such as betahCG and PSG PMID: 21799854
  53. Assessment of CGB and GNRH1 expression level in cancer patients' blood may be useful for indicating metastatic spread of tumor cells. PMID: 21827674
  54. EPO, hCG, or their combination ameliorate cardiac remodeling post-myocardial infarct in a rat model PMID: 21707946
  55. The presence of the uterine leiomyomas may increase maternal serum concentration of the beta-hCG and so after the rate of the false positive results of the prenatal screening tests. PMID: 21463216
  56. Serum hCG-beta level 12 days after embryo transfer is a useful predictor of subsequent presence of fetal cardiac activity and live birth after in vitro fertilization. PMID: 21719003
  57. Hyperemesis gravidarum is associated with elevated levels of PAPP-A and free beta-hCG, and such changes are independent of serum indicators of thyroid and liver function. PMID: 21360554
  58. Our data support the hypothesis that elevated second trimester maternal serum levels of total hCG and/or inhibin-A may result from premature accelerated differentiation of the villous cytotrophoblasts. PMID: 21388678
  59. Elevated serum and cerebrospinal fluid human chorionic gonadotropin-beta is associated with recurrent pure CNS germinoma. PMID: 20949592
  60. Raman and surface-enhanced Raman scattering (SERS) spectra of the synthetic carboxy terminal peptide of chorionic gonadatropin beta-subunit free of carbohydrate moieties(P37) are reported. PMID: 20872871
  61. [review] hCG's involvement in breast cancer PMID: 20654692
  62. Hyperglycosylated hCG promotes trophoblast invasion during choriocarcinoma, growth of cytotrophoblast cells, and placental implantation in pregnancy. PMID: 20619452
  63. Case Report: Mediastinal tumor diagnosed as thymic adenocarcinoma producing beta-hCG, and the tumor had originated from the thymus. PMID: 20421278
  64. This is first evidence of a monoclonal antibody that recognizes a peptide derived from chorionic gonadotropin (hCG)-beta antigen and is capable of suppressing tumor growth, both dose-dependently and in a major histocompatibility (HLA-A*0201) context. PMID: 20065111
  65. Data show that specific antibodies against purified beta-hCG forms had comparable titers and could be used in diagnostic kits. PMID: 19501580
  66. Isolation and purification using affinity capture MALDI-TOF MS was enhanced 3-fold by a method of orienting immunoglobulin G using immobilized recombinant Fc receptor protein G. PMID: 12175153
  67. Deleting the native acceptor sites exposes a new site for O-glycosylation and promotes a differential intracellular processing and folding of the beta subunit. PMID: 12242028
  68. Expression of the free beta-subunit of human chorionic gonadotropin in renal cell carcinoma: prognostic study on tissue and serum PMID: 12594819
  69. elevated b-HCG levels in our study were associated with pre-eclampsia.There is a statistically significant correlation between high levels of b-HCG and increased numbers of intrauterine growth restriction and newborns delivered in poor condition. PMID: 15048184
  70. Substitution of arginine(68) of the beta-subunit (hCG(beta)) with glutamic acid (R68E) profoundly reduces the cross-reactivity while refocusing the immune response to the hCG(beta)-specific C-terminal peptide (CTP). PMID: 15718290
  71. Overexpression of HCG beta is associated with adenoma formation PMID: 16007123
  72. HCGbeta is directly responsible for changes in prostate carcinoma cells associated with an increased metastatic phenotype PMID: 16323172
  73. In pertussis toxin-treated BeWo cells, 6-chloromelatonin stimulated basal hCG-beta secretion (P < 0.001). PMID: 16632463
  74. Kaplan-Meier plots and log-rank test revealed no significant difference in progression-free survival between patients with elevated or normal levels of serum and/or urinary total hCGbeta. PMID: 16651852
  75. hCGB suppresses breast cancer growth by triggering apoptosis via Bax and p53 pathways. PMID: 16924466
  76. The data presented here support the model of glycoprotein hormone-receptor interaction in which the hormone binds to the extracellular domain through the beta subunit and then the alpha subunit is brought in contact with the transmembrane domain. PMID: 17045394
  77. An intracellular intact hCG beta-subunit form that showed the transient expression of the hCG-beta-core fragment epitope beta11 in the course of the maturation of this subunit, is described. PMID: 17059865
  78. The increased hCGbeta concentrations in body fluids of patients with aggressive bladder cancer may be due to overexpression of type II genes. PMID: 17139196
  79. in patients with complex congenital malformations amniotic fluid levels of beta-hCG were higher than in controls PMID: 17141398
  80. Patients with preexisting systemic lupus erythematosus have increased maternal serum-free beta-hCG levels in the first-trimester. PMID: 17437322
  81. Data suggests that the expressional profile of CGB genes, activated already in blastocyst stage, is associated with the status of pregnancy. The presence of CGB transcripts in testes may indicate a role in male reproductive tract. PMID: 18048458
  82. LH/hCGR, coexpressed with hCGbeta, may act as a potential mediator of hCG action in nontrophoblastic gynecological cancers PMID: 18217981
  83. These data suggest that human chorionic gonadotropin beta subunit may act as a tumor growth-stimulating factor. PMID: 18339208
  84. Alpha-fetoprotein and human chorionic gonadotropin beta are elevated in high-grade neuroendocrine tumors with a rapidly progressive course and poorer survival. PMID: 18577995
  85. Elevated beta human chorionic gonadotrophin is associated with seminoma PMID: 18578862
  86. Expression correlates with reduced cervical squamous carcinoma cell apoptosis and may be involved in tumor vascularization and dissemination. PMID: 18752498
  87. Sensitivity of breast cancer cells to hormones that target beta-HCG may favor invasive breast cancer. PMID: 18758818
  88. Elevated beta-human chorionic gonadotropin is associated with primary central nervous system germ cell tumors. PMID: 18802946
  89. Early prediction of fetuses being SGA is feasible with the combination of first trimester PAPP-A, beta-hCG and ADAM12. PMID: 19003798
  90. Although, beta-hCG is expressed in the majority of pituitary adenomas, more especially in NFPA, it is un-related to the risk of tumour recurrence or cellular proliferation as measured by Ki-67 nuclear labelling. PMID: 19005764
  91. Median values in pregnant smokers versus nonsmokers does not affect diagnosis of Down syndrome in the first trimester. PMID: 19106261
  92. Secretory trafficking signal encoded in the carboxyl-terminal region of the CGbeta-subunit. PMID: 19131508
  93. maternal smoking associated with a reduction in cord blood beta-hCG PMID: 19290844
  94. beta-hCG has been found in a phyllodes tumor of the breast. PMID: 19624411

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Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Tissue Specificity High expression in the placenta throughout pregnancy.
Database Links

HGNC: 1886

OMIM: 118860

KEGG: hsa:1082

UniGene: Hs.172944

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