Human Chorionic Gonadotrophin,HCG ELISA Kit

Code CSB-E05060h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P0DN86
Lead Time 3-5 working days
Abbreviation hCG
Protein Biological Process 1 Sex hormone
Target Name Chorionic Gonadotrophin,HCG
Alias N/A
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, cell culture supernates
Detection Range 8 mIU/mL-240 mIU/mL
Sensitivity 2 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HCG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 86-97  
1:2 Average % 92  
Range % 85-99  
1:4 Average % 95  
Range % 92-100  
1:8 Average % 95  
Range % 89-98  
Recovery
The recovery of human HCG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 97 90-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average Corrected  
240 2.540 2.596 2.568 2.532  
100 1.279 1.299 1.289 1.253  
40 0.638 0.632 0.635 0.599  
16 0.285 0.291 0.288 0.252  
8 0.159 0.155 0.157 0.121  
0 0.036 0.035 0.036    
References
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Function Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role in pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Tissue Specificity High expression in the placenta throughout pregnancy.
Database Links

HGNC: 1886

OMIM: 118860

KEGG: hsa:1082

UniGene: Hs.172944

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