Human Cofilin-1(CFL1) ELISA kit

Instructions
Code CSB-EL005280HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cofilin 1 (non-muscle)
Alternative Names 18 kDa phosphoprotein ELISA Kit; CFL 1 ELISA Kit; CFL ELISA Kit; CFL1 ELISA Kit; COF1_HUMAN ELISA Kit; Cofilin 1 ELISA Kit; Cofilin 1 non muscle ELISA Kit; Cofilin ELISA Kit; Cofilin non muscle isoform ELISA Kit; Cofilin-1 ELISA Kit; epididymis secretory protein Li 15 ELISA Kit; HEL-S-15 ELISA Kit; non-muscle isoform ELISA Kit; p18 ELISA Kit
Abbreviation CFL1
Uniprot No. P23528
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CFL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 85-93
1:2 Average % 102
Range % 98-105
1:4 Average % 97
Range % 95-00
1:8 Average % 88
Range % 84-92
Recovery
The recovery of human CFL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 90-96
EDTA plasma (n=4) 105 101-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.604 2.638 2.621 2.486
800 2.078 2.041 2.060 1.925
400 1.329 1.305 1.317 1.182
200 0.804 0.797 0.801 0.666
100 0.431 0.456 0.444 0.309
50 0.294 0.287 0.291 0.156
25 0.213 0.206 0.210 0.075
0 0.136 0.134 0.135  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation
Gene References into Functions
  1. In conclusion, deregulation of cytoskeleton dynamics through TESK1/CFL1 pathway underlies epithelial intestinal dysfunction in the small bowel mucosa of diarrhea-predominant irritable bowel syndrome, particularly in female patients. PMID: 29396473
  2. High CFL1 expression is associated with malignant pancreatic lesions. PMID: 29347944
  3. High CFL1 expression is associated with epithelial-mesenchymal transition of gastric cancer. PMID: 28388575
  4. Cofilin-1 plays a dominant role in angiotensin-(1-7)-induced G0/G1 arrest and autophagy to maintain cellular homeostasis in human aortic endothelial cells PMID: 27748441
  5. NKCC1 not only controls cell volume and Cl- concentration, but it can also regulate the actin cytoskeleton through Cofilin 1. PMID: 28679472
  6. The enhancement of cancer cells invasion is dependent on CFL1, a known regulator of invadopodia maturation. PMID: 28446539
  7. The present study demonstrates that miR-145 plays an important role in inhibiting cell migration by directly targeting PAK4, and identifies miR-145-PAK4-LIMK1-cofilin as a novel regulatory pathway that contributed to colorectal cancer metastasis. PMID: 28440035
  8. Our results revealed that CKS1 is involved in normal glutamatergic synapse development and dendritic spine maturation in adult hippocampus through modulating p27 stability. PMID: 28365778
  9. Our study indicates that Cofilin 1 holds an important position in the development and progression of human bladder cancer PMID: 27768223
  10. Results suggest that the immune-complex (IC) of cofilin-1 in sera is a potentially attractive serum biomarker for the prognosis of pancreatic ductal adenocarcinoma (PDAC). PMID: 28161904
  11. High cofilin expression is associated with adrenocortical tumor. PMID: 28826686
  12. In advance urothelial cancer, overexpression of nuclear cofilin correlates with bladder cancer progression. PMID: 27402302
  13. Report structural basis for noncanonical substrate recognition of cofilin-1/LIMK1 to regulation actin cytoskeleton dynamics. PMID: 27153537
  14. STMN1,COF1 and PAIRBP1 thus represent proteins associated with proliferative and aggressive tumors of high grades, while TSP2 and POSTN were connected to low grade tumors with better prognosis PMID: 28216224
  15. We observed marked increases in LIM kinase 2 (LIMK2) and cofilin 1 (CFL1) gene expressions in metabolic syndrome patients. PMID: 26956845
  16. Therefore, miR-138/LIMK1/cofilin may be considered a potential therapeutic target for the treatment of non-small cell lung cancer PMID: 27665963
  17. These findings indicate that inhibition of the Rac1WAVE2Arp2/3 signaling pathway may promote radiosensitivity, which may partially result from the downregulation of CFL1 in U251 human glioma cells. PMID: 27052944
  18. The present findings demonstrated that the anticancer effect of docetaxel induces the apoptosis of prostate cancer via the suppression of the cofilin1 and paxillin signaling pathways, which will assist in setting a stage for the clinical treatment of prostate cancer. PMID: 27035282
  19. VEGFA induced phosphorylation of Pak1 and its effector cofilin in a manner that was dependent on time as well as p38MAPKbeta. PMID: 26857814
  20. CFL1 itself does not translocate actin into the cell nucleus but this transport requires the functional expression of IPO9. PMID: 26934847
  21. Abnormal CFL1 expression may affect vulvar carcinogenesis and subsequent progression. PMID: 26936386
  22. PAK1-cofilin phosphorylation mechanism to mediate lung adenocarcinoma cells migration promoted by apelin-13 PMID: 26918678
  23. Progesterone (P4) increases human astrocytoma cells migration and invasion through its intracellular receptor, and that cofilin activation by P4 is independent of progesterone receptor action. PMID: 26639431
  24. the presence of the actin-severing protein, cofilin, in human sperm, is reported. PMID: 26085344
  25. Aurora kinase A has a role in inducing papillary thyroid cancer lymph node metastasis by promoting cofilin-1 activity PMID: 27003257
  26. analysis of human Cof1, Cof2, and ADF effects on actin filament severing and turnover PMID: 26996939
  27. Cofilin 1 activation prevents the defects in axon elongation and guidance induced by extracellular alpha-synuclein PMID: 26558842
  28. novel roles for actin-depolymerizing factor and cofilin-1 in regulating the remodeling and permeability of epithelial junctions PMID: 26878213
  29. cofilin1 is involved in hypertensive nephropathy by modulating the nuclear translocation of NF-kappaB and the expression of its downstream inflammatory factors in renal tubular epithelial cells PMID: 26450610
  30. Cofilin-1 and transgelin may play roles in the carcinogenesis and development of esophageal squamous cell carcinoma PMID: 26344167
  31. results demonstrate that in VSMCs mechanical stimulation activates cofilin by a Nox4-dependent mechanism and that this pathway is required for cytoskeleton reorganization and cell reorientation PMID: 25998423
  32. Cor1B, Cof1 and AIP1 work in concert through a temporally ordered pathway to induce highly efficient severing and disassembly of actin filaments. PMID: 25995115
  33. the results of the present study suggested that miR-429 inhibits the migration and invasion of colon cancer cells, partly at least, by mediating the expression of PAK6, as well as the activity of cofilin signaling PMID: 26058485
  34. These results indicated that cofilin might be involved in the modulation of Aspergillus fumigatus internalization into type II alveolar epithelial cells through the RhoA-ROCK-LIM kinase pathway. PMID: 26268695
  35. Increasing radiosensitivity with the downregulation of cofilin-1 in U251 human glioma cells PMID: 25529407
  36. estradiol-17beta (E2beta) stimulates S-nitrosylation of CFL1 via eNOS-derived nitric oxide. PMID: 25635941
  37. Chronophin has a role in coordinating cell leading edge dynamics by controlling active cofilin levels PMID: 26324884
  38. cofilin-1 quantification in clinical samples in combination with presence/absence of EGFR mutation could be used to select patients that would benefit from TKI's treatment. PMID: 25784483
  39. Actin-modulating protein cofilin is involved in the formation of measles virus ribonucleoprotein complex at the perinuclear region. PMID: 26269174
  40. Novel signaling pathway consisting of chemoattractant GPCR/Galphai protein, PLC, PKCbeta and PKDs that regulates SSH2/cofilin activity, F-actin polymerization and directional assembly of actin cytoskeleton in neutrophil chemotaxis was identified. PMID: 25568344
  41. Overexpression of wt-cofilin-1 inhibited VEGF-induced F-actin formation PMID: 25056928
  42. Data indicate that knockdown of let-7b or let-7e could recover the growth rate and the invasion of cofilin-1 over-expressing cells. PMID: 25597880
  43. High-speed atomic force microscopy was employed to observe structural changes in actin filaments induced by cofilin binding. PMID: 25642645
  44. Data indicate that gamma-secretase is a negative regulator of Cofilin 1 activation. PMID: 25315299
  45. Actin filament severing by vertebrate cofilin is driven by the linked dissociation of a single, site-specific cation that controls filament structure and mechanical properties, and that filament severing is an essential function of cofilin in cells. PMID: 25468977
  46. ERK5 acted in concert with the actin remodeling protein, CFL1, and upon hormone exposure, both localized to active nuclear transcriptional hubs. PMID: 24505128
  47. We have also defined in the actin-binding protein cofilin-1 a link between PP2A, actin cytoskeleton, and necrotic death PMID: 25096578
  48. It provides a brief overview of the current knowledge about the central role of cofilin/actin rods formation, where its dysregulation and malfunction might be the trigger to neurodegeneration. PMID: 24813767
  49. Brain phosphorylated cofilin is downregulated in transgenic mice by RanBP9 overexpression, accelerating amyloid plaque accumulation. PMID: 24254706
  50. The CFL-1-dependent recruitment of actin to SPCA1 following calcium influx is critical for secretory cargo sorting. PMID: 25179631

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Subcellular Location Nucleus matrix, Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Peripheral membrane protein, Cytoplasmic side, Cell projection, lamellipodium membrane, Peripheral membrane protein, Cytoplasmic side, Cell projection, lamellipodium
Protein Families Actin-binding proteins ADF family
Tissue Specificity Widely distributed in various tissues.
Database Links

HGNC: 1874

OMIM: 601442

KEGG: hsa:1072

STRING: 9606.ENSP00000309629

UniGene: Hs.170622

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