Human Collagen alpha-1(X) chain (COL10A1) ELISA kit

Instructions
Code CSB-EL005715HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name collagen, type X, alpha 1
Alternative Names COAA1_HUMAN ELISA Kit; Col10a 1 ELISA Kit; COL10A1 ELISA Kit; Collagen alpha 1(X) chain ELISA Kit; Collagen alpha-1(X) chain ELISA Kit; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) ELISA Kit; Collagen type X alpha 1 ELISA Kit; Collagen X alpha 1 polypeptide ELISA Kit; CollagenX ELISA Kit; fa66d11 ELISA Kit; fb10c08 ELISA Kit; OTTHUMP00000040411 ELISA Kit; Procollagen type X alpha 1 ELISA Kit; Schmid metaphyseal chondrodysplasia ELISA Kit; wu:fa66d11 ELISA Kit; wu:fb10c08 ELISA Kit
Abbreviation COL10A1
Uniprot No. Q03692
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human COL10A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-106  
1:2 Average % 92  
Range % 88-95  
1:4 Average % 95  
Range % 90-101  
1:8 Average % 96  
Range % 91-103  
Recovery
The recovery of human COL10A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-101  
EDTA plasma (n=4) 92 87-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 3.039 3.052 3.046 2.909  
10 2.647 2.658 2.653 2.516  
5 1.898 2.040 1.969 1.832  
2.5 1.211 1.262 1.237 1.100  
1.25 0.708 0.663 0.686 0.549  
0.625 0.425 0.406 0.416 0.279  
0.312 0.242 0.257 0.250 0.113  
0 0.138 0.136 0.137    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
Gene References into Functions
  1. Plasma samples from lung cancer patients and healthy heavy-smokers controls were tested for levels of COL11A1 and COL10A1 (n = 57 each) and SPARC (n = 90 each). Higher plasma levels of COL10A1 were detected in patients (p PMID: 30227835
  2. All affected individuals are heterozygous for the missense mutation collagen type X alpha 1 chain (COL10A1) rs111033552. PMID: 29234170
  3. increased expression of stromal colXalpha1 and low TILs correlate with poor pathologic response in ER+/HER2+ breast tumors. Further studies are needed to confirm their predictive value and impact on long-term outcomes, and to determine whether this collagen exerts a protective effect on the cancer cells or simply reflects other factors within the tumor microenvironment PMID: 27090210
  4. a novel sequence variation involving an unusual mutational site of the COL10A1 gene can cause mild metaphyseal chondrodysplasia PMID: 25542771
  5. COL10A1 mutation 2005delC in a Chinese pedigree with Schmid type metaphyseal chondrodysplasia is close to the C-terminus of the protein sequence and may result in genetic heterogeneity of the Chinese population PMID: 25974987
  6. Concentration of serum collagen type X levels correlated with cartilage degradation in osteoarthritis patients. PMID: 25245039
  7. The results show that COL10A1 is a tumor biomarker upregulated in a wide variety of tumors including those of the breast, colon, bladder, stomach, esophagus, lung, testis, ovary and pancreas. PMID: 22894674
  8. Yiqi Huayu Bushen Recipe increased the expression of aggrecan, decreased the expression of type X collagen, and promoted cell proliferation in cells from degenerated human intervertebral discs. PMID: 22015197
  9. Genetic variation near FRK/COL10A1 and VEGFA are associated with advanced age-related macular degeneration. PMID: 21665990
  10. MCDS is a rare genetic skeletal disorder caused by a collagen type X defect. Though much is known about the molecular pathology of the causative COL10A1 mutations, causal therapy of the disease is not yet available PMID: 21360259
  11. a frameshift mutation leading to elongation of the deduced alpha1(X) chain associated with Metaphyseal Chondrodysplasia type Schmid PMID: 21447328
  12. These results indicate that nitrogen-rich plasma polymerized surfaces inhibit COL10A1 expression via the suppression of COX-1. PMID: 20225218
  13. speculate that complete loss of mutant transcripts yields COL10A1 haploinsufficiency and late clinical presentation while incomplete loss of mutant transcripts yields dominant-negative effects with early clinical presentation PMID: 20872587
  14. The total expression of type X collagen in the concave side growth plates of the lower end vertebrae was higher than that in the same side growth plates of apex. PMID: 20073986
  15. methylation-based COL10A1 gene silencing is established in cartilage tissue and human articular chondrocytes during chondrogenesis. PMID: 18759285
  16. chains harboring Schmid metaphyseal chondrodysplasia NC1 domain mutations are selectively retained and degraded in stably transfected cells PMID: 11805116
  17. The crystal structure at 2.0 A resolution of the human collagen X NC1 domain reveals an intimate trimeric assembly strengthened by a buried cluster of calcium ions. PMID: 11839302
  18. The 4.6 kb promoter is able to drive specific expression of Col10a1 in hypertrophic cartilage. PMID: 15464363
  19. exposure of the NC1 thiol may trigger the recognition and degradation of mutant collagen X chains PMID: 15695517
  20. The effect of COL10A1 nonsense mutations in cartilage tissue has been examined in two patients, demonstrating that the mutant mRNA is completely removed by nonsense mediated mRNA decay PMID: 15880705
  21. retinoids stimulate collagen X transcription IN chondrocytes PMID: 16598786
  22. the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation PMID: 16806867
  23. Type X collagen was not detected in any of atehrosclerotic plaques investigated in crural arteries. PMID: 17335825
  24. Investigated a family affected with the Spahr type of metaphyseal chondrodysplasia. Sequencing of RMRP, and a haplotype analysis using highly informative markers around the COL10A1 excluded both genes from being pathogenic in this family. PMID: 18553549
  25. HY(hypertrophy) box is the core element responsive to RUNX-2 in human COL10A1 promoter PMID: 19116917

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Involvement in disease Schmid type metaphyseal chondrodysplasia (SMCD)
Subcellular Location Secreted, extracellular space, extracellular matrix
Database Links

HGNC: 2185

OMIM: 120110

KEGG: hsa:1300

STRING: 9606.ENSP00000243222

UniGene: Hs.520339

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