Human Fatty-acid amide hydrolase 1(FAAH) ELISA kit

Code CSB-EL007938HU
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human FAAH ELISA Kit was designed for the quantitative measurement of Human FAAH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 34.4 pg/mL-2200 pg/mL and the sensitivity is 8.6 pg/mL.

Target Name fatty acid amide hydrolase
Alternative Names FAAH; FAAH1; Fatty-acid amide hydrolase 1; Anandamide amidohydrolase 1; Fatty acid ester hydrolase; Oleamide hydrolase 1
Abbreviation FAAH
Uniprot No. O00519
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 34.4 pg/mL-2200 pg/mL
Sensitivity 8.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FAAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 91-101  
1:2 Average % 103  
Range % 99-107  
1:4 Average % 93  
Range % 87-97  
1:8 Average % 99  
Range % 91-102  
Recovery
The recovery of human FAAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 84-94  
EDTA plasma (n=4) 94 88-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2200 2.489 2.413 2.451 2.332  
1100 2.117 2.098 2.108 1.989  
550 1.497 1.452 1.475 1.356  
275 0.948 0.939 0.944 0.825  
137.5 0.622 0.611 0.617 0.498  
68.75 0.375 0.368 0.372 0.253  
34.4 0.288 0.281 0.285 0.166  
0 0.121 0.117 0.119    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids.
Gene References into Functions
  1. the interaction between NAPE-PLD rs12540583 and FAAH rs324420, rs2295633 and rs6429600 is associated with schizophrenia. PMID: 29652995
  2. This study demonstrated that a significant association was observed between FAAH SNP genotype and self-report pain measures, mechanical and cold pain sensitivity among LBP participants. PMID: 28481838
  3. The FAAH levels were lower in the polycystic ovary syndrome(PCOS) group than the non-PCOS group. FAAH levels in secretory phases were significantly elevated compared to menstrual and proliferative phase. Dysregulation of the endocannabinoid system may result in PCOS. PMID: 28132572
  4. FAAH variants have shown a robust gene-environment interaction, namely, significantly higher anxiety and depression scores were exhibited by individuals carrying the A allele PMID: 27005594
  5. High FAAH expression is associated with lung neoplasms. PMID: 26930716
  6. study demonstrates a dose-dependent relationship between chronic cannabis use and reported sleep quality, independent of abstinence length. Furthermore, it provides novel evidence that depressive symptoms mediate the relationship between FAAH genotype and sleep quality in humans. PMID: 27074158
  7. FAAH was significantly associated with DSM-5 cannabis use disorder group count (DSM-5 CUD) using a gene-based test (p = 0.0035). This association survived Bonferroni correction for multiple testing at p < 0.004. Post hoc analyses suggested this association was driven by two common (minor allele frequency >5%) SNPs in moderate linkage disequilibrium, rs324420 and rs4141964, at p = 0.0014 and p = 0.0023, respectively. PMID: 27394933
  8. We first report loss-of-function mutations in DGAT2 and FAAH in one obese subject, which may interact with each other to affect the adiposity penetrance, providing a model of genetic interaction associated with human obesity. PMID: 28243972
  9. This study suggests that interactions between anandamide and CRF1 represent a fundamental molecular mechanism regulating amygdala function and anxiety. PMID: 26923505
  10. In cannabis users, fatty acid amide hydrolase binding was significantly lower across the brain regions examined than in matched control subjects. Lower binding was negatively correlated with cannabinoid concentrations in blood and urine and was associated with higher trait impulsiveness. PMID: 27345297
  11. We investigated the relationship between variation in the CNR1, CNR2, and FAAH genes and change in primary anxiety disorder severity. Five SNPs were nominally associated with a poorer treatment response (rs806365 [CNR1]; rs2501431 [CNR2]; rs2070956 [CNR2]; rs7769940 [CNR1]; rs2209172 [FAAH]) and one with a more favorable response (rs6928813 [CNR1]). PMID: 27346075
  12. C385A variation modulates stress responses in subjects with posttraumatic stress disorder and alcohol dependence PMID: 27716956
  13. REVIEW: role in pain and pain treatment PMID: 27240888
  14. the presence and differential distribution of fatty acid amide hydrolase (FAAH) and monoglyceride lipase (MGLL) in relation to CB1 during the maturation of human oocytes, was investigated. PMID: 26948343
  15. fatty acid amide hydrolase inhibition exerts cutaneous anti-inflammatory effects PMID: 26738935
  16. In transgenic mice, a direct involvement of the human FAAH C385A SNP was associated with alcohol "binge" drinking. PMID: 26857901
  17. Results suggest that carriers of FAAH A allele are at increased risk of Myocardial Infarction. PMID: 25270105
  18. FAAH gene variation was shown to associate with cold pain sensitivity with P129T/rs324420 being the most likely causal variant as it is known to reduce the FAAH enzyme activity. PMID: 26808012
  19. Data suggest that subjects who are carriers of minor allele (A) at missense mutation rs324420 in FAAH benefit from increased consumption of oleic and docosahexaenoic acids in dietary treatment of abdominal obesity. PMID: 26806592
  20. Specific FAAH polymorphisms are associated with refractory postoperative nausea and vomiting , opioid-related respiratory depression, and prolonged PACU stay due to opioid adverse effects in white children undergoing tonsillectomy. PMID: 25558980
  21. the FAAH 385C>A polymorphism may have a role in the endocannabinoid-related obesity phenotype PMID: 26561012
  22. Cannabis-using FAAH C/C carriers and A carrying controls have reduced white matter integrity compared to control C/C carriers. PMID: 26106535
  23. Studied the mechanism of FAAH for lipid selection to specifically hydrolyze anandamide, examining the role of the dynamic paddle formed at the boundaries of the FAAH catalytic site. PMID: 26111155
  24. FAAH genetic variation enhances fronto-amygdala function regarding fear regulation in mouse and human. PMID: 25731744
  25. no significant differences between preeclamptic and normal placenta in terms of CB2 and FAAH expressions and immunoreactivity PMID: 25444073
  26. evidence that the genetic variant rs324420 in FAAH is associated with measurable differences in brain FAAH binding as per PET [(11)C]CURB measurement. PMID: 26036940
  27. fatty acid amide hydrolase activity decreases in the frontal cortex from patients with Alzheimer's disease PMID: 25456842
  28. High FAAH expression is associated with drug resistance in non-small cell lung cancer. PMID: 24811863
  29. In subcutaneous adipose tissue, DAGL-a mRNA was upregulated and fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) mRNAs were down-regulated in obese subjects, but the diets had no influence. PMID: 24616451
  30. Single nucleotide polymorphism in the FAAH gene was not associated with IBS pathogenesis. PMID: 24444427
  31. FAAH Pro129/Pro129 homozygotes, reported higher placebo analgesia and more positive affective states immediately and 24 h after placebo administration; no effects on pain report in the absence of placebo were observed. PMID: 24042479
  32. It controls intestinal motility through its putative receptors or non-receptor-mediated pathways. Thus, Decreased enteric fatty acid amide hydrolase activity is associated with colonic inertia in slow transit constipation. PMID: 23926887
  33. Subjects with C385C genotype of the FAAH showed an improvement on insulin and HOMA-R levels with a high polyunsaturated fat hypocaloric diet after weight loss during 3 months PMID: 24445122
  34. In obese humans, FAAH or MGL activity in adipocytes is not affected by diabetes, dyslipidaemia or other markers of metabolic dysfunction. PMID: 24593280
  35. FLAT does not serve as a global intracellular AEA carrier PMID: 24223930
  36. Suggest anandamide and FAAH as direct modifiers in signalling mediated activation of Nrf2-HO-1 pathway. PMID: 23347118
  37. our results indicate that the FAAH C385A SNP is one of the most promising candidates for individuals who are at higher risk for alcohol problems. PMID: 24407958
  38. The findings support an association with CNR1 and FAAH genes and marijuana-related problems among regular marijuana users PMID: 24172113
  39. the C385A polymorphism of the fatty acid amide hydrolase gene is not a genetic susceptibility factor for the development of polycystic ovary syndrome. PMID: 23616186
  40. genetic association study in population in Spain: Data suggest SNP in FAAH (C385A, rs324420) is associated with response of obese subjects to reducing diet which is high in monounsaturated fats; weight loss is higher in subjects with C385C genotype. PMID: 23333123
  41. Our findings suggest that the FAAH Pro129Thr polymorphism may contribute to methamphetamine dependence in the Malay and Chinese ethnic groups PMID: 23556448
  42. The differential expression of NAPE-PLD and FAAH suggests that Anandamide could play an important role in the pathophysiology of preeclampsia. PMID: 23122699
  43. FAAH A carriers, compared to C/C carriers, exhibited increased startle potentiation and emotional responsiveness towards unpleasant picture stimuli, and reduced startle inhibition indicating reduced emotional reactivity in response to pleasant pictures PMID: 22776995
  44. Elevated endocannabinoid levels and reduced FAAH activity are associated with ectopic pregnancy and may modulate tubal function, suggesting dysfunctional endocannabinoid action in ectopic implantation. PMID: 23372171
  45. Mapping human brain fatty acid amide hydrolase activity with PET. PMID: 23211960
  46. FAAH expression increase steadily after infancy, peaking in adulthood. PMID: 22827915
  47. our data suggest that FAAH has an important role in PTSD through modulation of aversive memories and point to both a novel therapeutic target and a possible risk marker. PMID: 22832737
  48. genetic association studies in a population in Spain: Data suggest that an SNP in FAAH (rs324420) is not associated with response/resistance or remission/no remission with citalopram treatment in patients with major depressive disorder. PMID: 22826533
  49. There is a lack of association of the FAAH genotypes with metabolic syndrome prevalence. PMID: 22609216
  50. Cannabinoid receptors and endocannabinoid modulating enzymes were localized in fallopian tube epithelium by immunohistochemistry and showed reduced CB1 and FAAH expression in ectopic pregnancy PMID: 22701012

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Subcellular Location Endomembrane system; Single-pass membrane protein. Cytoplasm, cytoskeleton. Note=Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.
Protein Families Amidase family
Tissue Specificity Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.
Database Links

HGNC: 3553

OMIM: 602935

KEGG: hsa:2166

STRING: 9606.ENSP00000243167

UniGene: Hs.720143

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