Product Details

Uniprot No.

Target Names

FAAH

Alternative Names

FAAH; FAAH1; Fatty-acid amide hydrolase 1; Anandamide amidohydrolase 1; Fatty acid ester hydrolase; Oleamide hydrolase 1

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human FAAH1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8G3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Fatty acid amide hydrolase (FAAH) serves as the primary enzyme responsible for degrading endocannabinoid signaling molecules, including anandamide and oleamide. By regulating the tone of the endocannabinoid system, FAAH influences diverse physiological processes spanning pain perception, inflammation, mood regulation, and metabolic homeostasis. This central role in lipid signaling has positioned FAAH as a compelling target for researchers investigating cancer biology, metabolic disorders, and signal transduction pathways.

This recombinant monoclonal antibody, clone 8G3, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in a controlled recombinant system, you can expect reliable performance across experiments and between lots, eliminating the variability that often complicates long-term studies or multi-site collaborations.

Validation testing demonstrates robust performance across multiple applications. In western blot experiments, the antibody detects FAAH at the expected 64 kDa molecular weight across a diverse panel of human cell lines including MCF-7, THP-1, HEK293, and HepG2, as well as mouse liver tissue, confirming cross-species reactivity between human and mouse samples. This breadth of validated sample types provides flexibility whether you're working with cancer cell models, immune cells, or primary tissue lysates. For tissue localization studies, immunohistochemistry validation in paraffin-embedded human testis tissue demonstrates clear specific staining, supporting applications in archival tissue analysis.

The unconjugated format and affinity-purified preparation allow straightforward integration into existing detection systems. Whether you're mapping FAAH expression patterns in tumor samples, investigating metabolic regulation, or characterizing endocannabinoid pathway dynamics, this antibody delivers the specificity and consistency your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids.

Gene References into Functions

the interaction between NAPE-PLD rs12540583 and FAAH rs324420, rs2295633 and rs6429600 is associated with schizophrenia. PMID: 29652995
This study demonstrated that a significant association was observed between FAAH SNP genotype and self-report pain measures, mechanical and cold pain sensitivity among LBP participants. PMID: 28481838
The FAAH levels were lower in the polycystic ovary syndrome(PCOS) group than the non-PCOS group. FAAH levels in secretory phases were significantly elevated compared to menstrual and proliferative phase. Dysregulation of the endocannabinoid system may result in PCOS. PMID: 28132572
FAAH variants have shown a robust gene-environment interaction, namely, significantly higher anxiety and depression scores were exhibited by individuals carrying the A allele PMID: 27005594
High FAAH expression is associated with lung neoplasms. PMID: 26930716
study demonstrates a dose-dependent relationship between chronic cannabis use and reported sleep quality, independent of abstinence length. Furthermore, it provides novel evidence that depressive symptoms mediate the relationship between FAAH genotype and sleep quality in humans. PMID: 27074158
FAAH was significantly associated with DSM-5 cannabis use disorder group count (DSM-5 CUD) using a gene-based test (p = 0.0035). This association survived Bonferroni correction for multiple testing at p < 0.004. Post hoc analyses suggested this association was driven by two common (minor allele frequency >5%) SNPs in moderate linkage disequilibrium, rs324420 and rs4141964, at p = 0.0014 and p = 0.0023, respectively. PMID: 27394933
We first report loss-of-function mutations in DGAT2 and FAAH in one obese subject, which may interact with each other to affect the adiposity penetrance, providing a model of genetic interaction associated with human obesity. PMID: 28243972
This study suggests that interactions between anandamide and CRF1 represent a fundamental molecular mechanism regulating amygdala function and anxiety. PMID: 26923505
In cannabis users, fatty acid amide hydrolase binding was significantly lower across the brain regions examined than in matched control subjects. Lower binding was negatively correlated with cannabinoid concentrations in blood and urine and was associated with higher trait impulsiveness. PMID: 27345297
We investigated the relationship between variation in the CNR1, CNR2, and FAAH genes and change in primary anxiety disorder severity. Five SNPs were nominally associated with a poorer treatment response (rs806365 [CNR1]; rs2501431 [CNR2]; rs2070956 [CNR2]; rs7769940 [CNR1]; rs2209172 [FAAH]) and one with a more favorable response (rs6928813 [CNR1]). PMID: 27346075
C385A variation modulates stress responses in subjects with posttraumatic stress disorder and alcohol dependence PMID: 27716956
REVIEW: role in pain and pain treatment PMID: 27240888
the presence and differential distribution of fatty acid amide hydrolase (FAAH) and monoglyceride lipase (MGLL) in relation to CB1 during the maturation of human oocytes, was investigated. PMID: 26948343
fatty acid amide hydrolase inhibition exerts cutaneous anti-inflammatory effects PMID: 26738935
In transgenic mice, a direct involvement of the human FAAH C385A SNP was associated with alcohol "binge" drinking. PMID: 26857901
Results suggest that carriers of FAAH A allele are at increased risk of Myocardial Infarction. PMID: 25270105
FAAH gene variation was shown to associate with cold pain sensitivity with P129T/rs324420 being the most likely causal variant as it is known to reduce the FAAH enzyme activity. PMID: 26808012
Data suggest that subjects who are carriers of minor allele (A) at missense mutation rs324420 in FAAH benefit from increased consumption of oleic and docosahexaenoic acids in dietary treatment of abdominal obesity. PMID: 26806592
Specific FAAH polymorphisms are associated with refractory postoperative nausea and vomiting , opioid-related respiratory depression, and prolonged PACU stay due to opioid adverse effects in white children undergoing tonsillectomy. PMID: 25558980
the FAAH 385C>A polymorphism may have a role in the endocannabinoid-related obesity phenotype PMID: 26561012
Cannabis-using FAAH C/C carriers and A carrying controls have reduced white matter integrity compared to control C/C carriers. PMID: 26106535
Studied the mechanism of FAAH for lipid selection to specifically hydrolyze anandamide, examining the role of the dynamic paddle formed at the boundaries of the FAAH catalytic site. PMID: 26111155
FAAH genetic variation enhances fronto-amygdala function regarding fear regulation in mouse and human. PMID: 25731744
no significant differences between preeclamptic and normal placenta in terms of CB2 and FAAH expressions and immunoreactivity PMID: 25444073
evidence that the genetic variant rs324420 in FAAH is associated with measurable differences in brain FAAH binding as per PET [(11)C]CURB measurement. PMID: 26036940
fatty acid amide hydrolase activity decreases in the frontal cortex from patients with Alzheimer's disease PMID: 25456842
High FAAH expression is associated with drug resistance in non-small cell lung cancer. PMID: 24811863
In subcutaneous adipose tissue, DAGL-a mRNA was upregulated and fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) mRNAs were down-regulated in obese subjects, but the diets had no influence. PMID: 24616451
Single nucleotide polymorphism in the FAAH gene was not associated with IBS pathogenesis. PMID: 24444427
FAAH Pro129/Pro129 homozygotes, reported higher placebo analgesia and more positive affective states immediately and 24 h after placebo administration; no effects on pain report in the absence of placebo were observed. PMID: 24042479
It controls intestinal motility through its putative receptors or non-receptor-mediated pathways. Thus, Decreased enteric fatty acid amide hydrolase activity is associated with colonic inertia in slow transit constipation. PMID: 23926887
Subjects with C385C genotype of the FAAH showed an improvement on insulin and HOMA-R levels with a high polyunsaturated fat hypocaloric diet after weight loss during 3 months PMID: 24445122
In obese humans, FAAH or MGL activity in adipocytes is not affected by diabetes, dyslipidaemia or other markers of metabolic dysfunction. PMID: 24593280
FLAT does not serve as a global intracellular AEA carrier PMID: 24223930
Suggest anandamide and FAAH as direct modifiers in signalling mediated activation of Nrf2-HO-1 pathway. PMID: 23347118
our results indicate that the FAAH C385A SNP is one of the most promising candidates for individuals who are at higher risk for alcohol problems. PMID: 24407958
The findings support an association with CNR1 and FAAH genes and marijuana-related problems among regular marijuana users PMID: 24172113
the C385A polymorphism of the fatty acid amide hydrolase gene is not a genetic susceptibility factor for the development of polycystic ovary syndrome. PMID: 23616186
genetic association study in population in Spain: Data suggest SNP in FAAH (C385A, rs324420) is associated with response of obese subjects to reducing diet which is high in monounsaturated fats; weight loss is higher in subjects with C385C genotype. PMID: 23333123
Our findings suggest that the FAAH Pro129Thr polymorphism may contribute to methamphetamine dependence in the Malay and Chinese ethnic groups PMID: 23556448
The differential expression of NAPE-PLD and FAAH suggests that Anandamide could play an important role in the pathophysiology of preeclampsia. PMID: 23122699
FAAH A carriers, compared to C/C carriers, exhibited increased startle potentiation and emotional responsiveness towards unpleasant picture stimuli, and reduced startle inhibition indicating reduced emotional reactivity in response to pleasant pictures PMID: 22776995
Elevated endocannabinoid levels and reduced FAAH activity are associated with ectopic pregnancy and may modulate tubal function, suggesting dysfunctional endocannabinoid action in ectopic implantation. PMID: 23372171
Mapping human brain fatty acid amide hydrolase activity with PET. PMID: 23211960
FAAH expression increase steadily after infancy, peaking in adulthood. PMID: 22827915
our data suggest that FAAH has an important role in PTSD through modulation of aversive memories and point to both a novel therapeutic target and a possible risk marker. PMID: 22832737
genetic association studies in a population in Spain: Data suggest that an SNP in FAAH (rs324420) is not associated with response/resistance or remission/no remission with citalopram treatment in patients with major depressive disorder. PMID: 22826533
There is a lack of association of the FAAH genotypes with metabolic syndrome prevalence. PMID: 22609216
Cannabinoid receptors and endocannabinoid modulating enzymes were localized in fallopian tube epithelium by immunohistochemistry and showed reduced CB1 and FAAH expression in ectopic pregnancy PMID: 22701012

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Subcellular Location

Endomembrane system; Single-pass membrane protein. Cytoplasm, cytoskeleton. Note=Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.

Protein Families

Amidase family

Tissue Specificity

Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.

Database Links

HGNC: 3553

OMIM: 602935

KEGG: hsa:2166

STRING: 9606.ENSP00000243167

UniGene: Hs.720143

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Code	CSB-RA560252A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Mouse Liver whole cell lysate All lanes: FAAH1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa IHC image of CSB-RA560252A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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FAAH Recombinant Monoclonal Antibody

Product Details

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Target Background