Human Interleukin 28A,IL-28A/IFN-λ2 ELISA kit

Instructions
Code CSB-E11708h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Interleukin 28A,IL-28A/IFN-λ2
Alternative Names Cytokine ZCYTO20 ELISA Kit; IFN-lambda-2 ELISA Kit; IFNL2 ELISA Kit; IL-28A ELISA Kit; IL28A ELISA Kit; IL28A_HUMAN ELISA Kit; Interferon lambda 2 ELISA Kit; Interferon lambda-2 ELISA Kit; interleukin 28A (interferon, lambda 2) ELISA Kit; Interleukin 28A ELISA Kit; Interleukin-28A ELISA Kit; ZCYTO20 ELISA Kit
Abbreviation IL-28A/IFN-λ2
Uniprot No. Q8IZJ0
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-28A/IFN-λ2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 104    
Range % 100-108    
1:2 Average % 95    
Range % 90-100    
1:4 Average % 90    
Range % 85-94    
1:8 Average % 98    
Range % 94-102    
Recovery
The recovery of human IL-28A/IFN-λ2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 84-93    
EDTA plasma (n=4) 92 88-96    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
2000 2.815 2.742 2.779 2.680    
1000 2.352 2.196 2.274 2.175    
500 1.673 1.609 1.641 1.542    
250 0.961 1.004 0.983 0.884    
125 0.527 0.561 0.544 0.445    
62.5 0.289 0.295 0.292 0.193    
31.25 0.204 0.213 0.209 0.110    
0 0.096 0.101 0.099      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets
Gene References into Functions
  1. NS of severe fever with thrombocytopenia syndrome virus inhibited the activity of IFN-alpha1, IFN-beta, IFN-lambda1 and IFN-lambda2 through inhibition of STAT1 phosphorylation. PMID: 26353965
  2. Studies indicate that the type III interferons (IFNs) or IFN-lambdas consists of four members: IFN-lambda1 (IL-29), IFN-lambda2 (IL28A), IFN-lambda3 (IL-28B) and IFN-lambda4. PMID: 26194286
  3. These results show that type III interferons (IFN-lambdas) play a critical protective role in human metapneumovirus infection. PMID: 25355870
  4. Results showed no association between genotypes and alleles of IL28A, IL28B or IL29 polymorphisms and Hepatitis C virus infection. PMID: 24269996
  5. this study provides evidence for potential IL-28A participation in Behcet's diseaseand its value as a therapeutic agent. PMID: 24973639
  6. Interferon regulatory factor (IRF)-3 and -7 are the key transcriptional factors for the induction of IL-28A and IL-28B genes, whereas NF-kappaB is an additional requirement for the induction of the IL-29 gene. PMID: 24385435
  7. The results show that IL-28A/IL-28AR1 dyad-induced wound healing migration requires NF-kappaB-mediated MMP-9 expression by MAPK activation. PMID: 22560878
  8. IL-28 genotype was not associated with response to interferon treatment (OR for GT/GG vs. TT, 0.881 (95%CI 0.388 - 2.002); P = 0.762; OR for CT/CC vs. TT, 0.902 (95%CI 0.458 - 1.778); P = 0.766). PMID: 23652058
  9. IFN-lambdas can also directly affect T cells through inhibition of the T helper 2 cell (Th2) responses. IFN-lambdas have varying immunomodulatory functions under different physiological conditions PMID: 23207147
  10. p38 MAPK pathway mediates IL-28A-induced cell migration through MMP-9 expression by activating NF-kappaB and AP-1 binding motifs. PMID: 22825757
  11. Elevated expression of inflammatory cytokines (IL-5, IL-20, and IL-28A) is associated with bladder cancer development. PMID: 22962576
  12. Plasmacytoid dendritic cells are major producers of IFN-lambda2 (a type III interferon) in response to viral stimulation. PMID: 22891284
  13. This study aimed to 1) examine DNA sequences in IL28B surrounding each of the reported associated single nucleotide polymorphisms and the corresponding regions in IL28A. PMID: 22253847
  14. IL-29/IL-28A suppress herpes simplex virus type 1 (HSV-1) infection of human NT2-N neurons. PMID: 21499846
  15. In general IL-29 serum levels exceeded IL-28A/B at least twofold, with IL-29 and IL-28A/B levels being significantly higher in carriers of the rs12979860 C allele than in TT homozygous individuals in hepatitis C PMID: 21145813
  16. In contrast to IL-29, IL-28A is a potent gene repressor in patients with hepatitis C PMID: 21170333
  17. Observational study of gene-disease association. (HuGE Navigator) PMID: 20576307
  18. Chronic hepatitis C patients with the rs12979860 CC responder genotype may have a lower endogenous IFN response to the virus. PMID: 20235331
  19. study supports that IFN-lambdas do not influence every type of cell and that membrane-associated variant IFN-lambda R1 expression is not sufficient to ensure cellular sensitivity toward these cytokines. PMID: 19798076
  20. identified from the human genomic sequence a family of three cytokines, designated interleukin 28A (IL-28A), IL-28B and IL-29, that are distantly related to type I interferons (IFNs) and the IL-10 family PMID: 12469119
  21. closely positioned genes on human chromosome 19 encode distinct but paralogous proteins, which we designate interferon-lambda1 (IFN-lambda1), IFN-lambda2 and IFN-lambda3 PMID: 12483210
  22. IL-28A and IL-29 induced mRNA expression of the antiviral proteins 2',5'-OAS and MxA was abolished by overexpression of SOCS-1 PMID: 15850793
  23. we found IL-28A and IL-29 act similarly to IFNs, but are less effective generally and have activity in a more limited range of cell lines PMID: 15899585
  24. IECs express functional receptors for IFN-lambdas, which mediate antiviral and antiproliferative signals in IECs, suggesting a potential for therapeutic use in certain viral infections and as (antiproliferative) anticancer therapy PMID: 16051921
  25. IL-28A antiviral activity is associated with the activation of the JAK-STAT signaling pathway and expression of ISGs. PMID: 16146571
  26. IFN-lambdas are thus able to generate tolerogenic DCs, an activity that could thwart IFN-beta functions PMID: 16478884
  27. IFNs lambda exhibit several common features with type I IFNs: antiviral activity, antiproliferative activity and in vivo antitumour activity [review] PMID: 17367910
  28. These data provide direct and compelling evidence that IFN-lambda, through both extracellular and intracellular antiviral mechanisms, inhibits HIV-1 replication in macrophages. PMID: 19193806
  29. recent findings about the biology of IFN-lambdas and their pathophysiological roles in viral infection, cancer, and immune responses of the innate and adaptive arms.[review] PMID: 19304895

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Subcellular Location Secreted
Protein Families Lambda interferon family
Database Links

HGNC: 18364

OMIM: 607401

KEGG: hsa:282616

STRING: 9606.ENSP00000333639

UniGene: Hs.567792

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