Human Metastasis-associated in colon cancer protein 1(MACC1) ELISA kit

Code CSB-EL013298HU
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This human MACC1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human MACC1 in multiple samples, including serum, plasma, tissue homogenates, or cell lysates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the MACC1 levels in the sample and is measured at 450 nm through a microplate reader.

MACC1 has been reported to be overexpressed in multiple cancers including glioblastoma multiforme and gastric cancer and promotes proliferation, metastasis, cancer stem cell-like properties, and drug resistance of cancer cells. It is a potent prognostic biomarker inducing proliferation, migration, invasiveness, and metastasis of cancer cells. MACC1 promotes carcinogenesis, growth, and metastasis of colorectal cancer. Furthermore, MACC1 is linked to the promotion of tumor growth, invasion, and metastasis in gastric cancer, as well as poor prognosis in solid cancers.

Target Name metastasis associated in colon cancer 1
Alternative Names 7A5 ELISA Kit; MACC1 ELISA Kit; MACC1_HUMAN ELISA Kit; Metastasis associated in colon cancer 1 ELISA Kit; Metastasis-associated in colon cancer protein 1 ELISA Kit; OTTHUMP00000201891 ELISA Kit; Putative binding protein 7a5 ELISA Kit; SH3 domain-containing protein 7a5 ELISA Kit; SH3BP4L ELISA Kit
Abbreviation MACC1
Uniprot No. Q6ZN28
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MACC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 92-101
1:2 Average % 84
Range % 80-88
1:4 Average % 91
Range % 85-95
1:8 Average % 98
Range % 94-102
Recovery
The recovery of human MACC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 106 101-113
EDTA plasma (n=4) 86 82-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.711 2.541 2.626 2.512
1500 1.955 1.874 1.915 1.801
750 1.044 1.105 1.075 0.961
375 0.588 0.612 0.600 0.486
187.5 0.353 0.340 0.347 0.233
94 0.236 0.253 0.245 0.131
47 0.178 0.169 0.174 0.060
0 0.111 0.117 0.114  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo.
Gene References into Functions
  1. Expression levels of MACC1, CD44, Twist1, and KiSS-1 are related to duration of overall survival among patients with colonic adenocarcinoma. PMID: 30021598
  2. Authors validated that metastasis associated in colon cancer-1 (MACC1) is a novel target of miR-598 in GBM. Restoring MACC1 expression reversed the inhibitory effects of miR-598 overexpression on GBM cells. PMID: 29444745
  3. These results demonstrated that miR485 may perform its tumor suppressive function in cervical cancer by directly targeting MACC1 and inhibiting the Met/AKT signaling pathway. Therefore, the miR485/MACC1 axis may be a novel and effective therapeutic target in cervical cancer. PMID: 29916552
  4. MACC1 is an independent prognostic marker in patients with small invasive lung adenocarcinoma after complete surgical resection. Differential outcomes are associated with MACC1 expression level. PMID: 29630522
  5. MACC1 and c-Myc are highly expressed in serum and tumor tissues of EC patients. Both are correlated with TNM stage, primary infiltration, and lymph node or distal metastasis. PMID: 29984790
  6. The results of this study found that MACC1 protein expression in tumor tissue is significantly prognostic for shorter disease-free survival. PMID: 29678846
  7. miR-338-3p/MACC1/MAPK regulatory pathway play an important role in the progression of cervical cancer. PMID: 29243777
  8. this study found that upregulation of MACC1 in ESCC was associated with lymph node metastasis of patients, and MACC1 regulated ESCC cell proliferation, apoptosis, migration and invasion mainly through AMPK-ULK1 induced autophagy PMID: 28791376
  9. MACC1 overexpression is associated with poor survival rates, regional invasion and lymph-node metastasis [meta-analysis] PMID: 27542234
  10. MACC1 expression stratifies colon cancer patients with unfavorable pMMR status. Stage II colon cancer patients with pMMR/MACC1-low tumors have a similar favorable prognosis to those with dMMR with potential implications for the role of adjuvant therapy PMID: 28460000
  11. coexistence of high MACC1 and low NM23-H1 expression and tumor budding was associated with short overall survival PMID: 29700912
  12. YB-1 promoted lung adenocarcinoma growth and progression in vitro and in vivo through directly binding to the MACC1 promoter and enhancing MACC1/c-Met pathway. PMID: 28624808
  13. Serum metastasis-associated in colon cancer-1 (MACC1) levels were higher in breast cancer patients than patients with benign disease or healthy volunteers. PMID: 27793048
  14. restoration of MACC1 expression could abrogate the anti-metastatic effects of miR-944 on colorectal cancer (CRC)cells with enhanced cell migration and invasion. MACC1/Met/AKT signaling may be implicated with the function of miR-944 in CRC cells. Altogether, miR-944 potentially act as a prognostic predictor and a drug-target for CRC patients. PMID: 28498456
  15. Data suggest that the miR-497/MACC1 axis mediated the effect of XIST on GC cell growth. PMID: 27911852
  16. these findings highlight the pivotal role of miR-218 in various aspects of MACC1 expression regulation and MACC1-mediated colorectal cancer progression PMID: 27462788
  17. MACC1-induced tumor progression in colorectal cancer acts, at least in part, via the newly discovered MACC1/Nanog/Oct4 axis. PMID: 26758557
  18. Data indicate that OR3A4 upregulation contributes to metastasis and tumorigenesis in gastric cancer by regulating the activation of PDLIM2, MACC1, NTN4, and GNB2L1. PMID: 26863570
  19. H19 may regulate non-small cell lung cancer (NSCLC) metastasis through modulating cellular signaling pathway proteins related to cell proliferation and cell adhesion, including MACC1, EGFR, beta-catenin and ERK1/2. PMID: 27607135
  20. Metastasis-associated in colon cancer 1 is expressed at early stages of colorectal oncogenesis within the affected colonic tissue in colorectal cancer patient cohort. PMID: 27439755
  21. MACC1 is a key driver and prognostic biomarker for cancer progression and metastasis in a large variety of solid tumor types, particularly colorectal cancer (CRC). Data suggest that some small molecules with antineoplastic activity inhibit MACC1 transcription in CRC and prevent liver metastasis in CRC; lovastatin and rottlerin are the antineoplastic agents used here. PMID: 28570591
  22. MACC1 regulates Fas mediated apoptosis through STAT1/3 - Mcl-1 signaling in solid cancers. PMID: 28649004
  23. High expression of MACC1 is associated with non-small cell lung cancer. PMID: 27832750
  24. MACC1 down-regulation suppresses endometrial carcinoma cell proliferation. PMID: 27991682
  25. SPON2 is a transcriptional target of the metastasis gene MACC1. SPON2 induces cell motility in vitro and CRC metastasis in mice. In patients, SPON2 serves as prognostic indicator for CRC metastasis and survival, and might represent a promising target for therapeutic approaches. PMID: 26686083
  26. High MACC1 expression is associated with Angiogenesis and recurrence in Gastric Cancer. PMID: 27280289
  27. Elevated Orai1 and STIM1 expressions upregulate MACC1 expression to promote tumor cell proliferation, metabolism, migration, and invasion in human gastric cancer. PMID: 27431311
  28. Data show that metastasis-associated in colon cancer-1 (MACC1) and monocarboxylate transporter 1 (MCT1) are highly expressed in gastric cancer indicating poor prognosis. PMID: 28235486
  29. A novel role of MACC1 in gastric cancer cell lipogenesis.MACC1 and FASN are positively correlated and responsible for the poor prognoses in gastric cancer patients. PMID: 28339092
  30. MACC1 is more frequently expressed in a poor prognosis phenotype of gastric cancer and acts as a promising prognostic prediction parameter for gastric cancer PMID: 27143263
  31. Results indicate that Nav 1.7 promotes GC progression through MACC1-mediated upregulation of NHE1. PMID: 27529686
  32. MACC1 gene (metastasis-associated in colon cancer 1) is upregulated in cancer stem cells both resistant and sensitive to chemotherapy PMID: 27917797
  33. MACC1 overexpression is associated with gastric cancer. PMID: 27793161
  34. High MACC1 expression is associated with colorectal cancer. PMID: 27492459
  35. Downregulation of MACC1 upregulates E-cadherin, N-cadherin and Vimentin in melanoma cell lines. PMID: 27488539
  36. MACC1 is essential for acetylcholine-induced gastric cancer cell invasion/migration and epithelial-mesenchymal transition PMID: 26919111
  37. Study demonstrated that suppression of MACC1 improves the chemosensitivity of cisplatin in epithelial ovarian cancer cells probably through regulation of the ERK1/2 signaling pathway and P-gp and its downstream apoptotic proteins. PMID: 26794135
  38. MACC1 was a specific target gene of miR-338-3p, which regulates malignant biological behaviors of glioma cells partly through directly silencing MACC1 expression. PMID: 26936749
  39. This study suggests that MACC1 is an independent prognostic factor in gastric adenocarcinoma and that the prognostic impact of MACC1 may be associated with MACC1 partners other than MET. PMID: 26719224
  40. Metastasis-associated in colon cancer-1 may have an important role in tumour development in adenoid cystic carcinoma. Metastasis-associated in colon cancer-1 is a potential biomarker for adenoid cystic carcinoma. PMID: 25640194
  41. downregulation of MACC1 expression could enhance cisplatin sensitivity and decrease drug resistance in SKOV- 3/DDP cells. PMID: 26681060
  42. Our findings suggest that MACC1 protein, as a valuable marker of cervical cancer prognosis, plays an important role in the progression of human cervical cancer cells. PMID: 26332389
  43. Our results suggest that rs975263 and rs4721888 polymorphisms in MACC1 are associated with the risk of breast cancer susceptibility and may be involved in the progression of breast cancer in Chinese women PMID: 26871844
  44. Conclude that the ability of proliferation and invasion in T24 cells can be inhibited by RNAi-targeting MACC1. PMID: 26339359
  45. the expression levels of MACC1 were significantly correlated with the biological processes underlying glioma cell proliferation, invasion and metastasis. PMID: 26043756
  46. In renal cell carcinoma, positive MACC1 expression was found to significantly correlate with distant metastasis and TNM stage. patients with higher MACC1 expression had a significantly lower disease-free rate PMID: 26097569
  47. conclusion, high MACC1 expression may serve as a prognostic biomarker to guide individualized management in clinical practice for digestive system neoplasms. PMID: 26090393
  48. MACC1 promotes vasculogenic mimicry in gastric cancer by regulating the HGF/c-Met-TWIST1/2 signaling pathway. PMID: 25895023
  49. MACC1 expression predicts survival outcome in colorectal cancer patients. PMID: 25884643
  50. These results suggested that MACC1 leads to a poor prognosis in HCC, partly by promoting proliferation via enhancement in glucose metabolism by HK2. PMID: 25738944

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Subcellular Location Cytoplasm. Nucleus. Note=Mainly found in the cytoplasm in non-metastasizing tumors.
Tissue Specificity Preferentially expressed in metastasizing tumors.
Database Links

HGNC: 30215

OMIM: 612646

KEGG: hsa:346389

STRING: 9606.ENSP00000328410

UniGene: Hs.598388

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