Human Netrin-1,Ntn1 ELISA Kit

Code CSB-E11899h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human NTN1 ELISA Kit was designed for the quantitative measurement of Human NTN1 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Name netrin 1
Alternative Names epididymis tissue protein Li 131P ELISA Kit; NET1_HUMAN ELISA Kit; Netrin 1, mouse, homolog of ELISA Kit; Netrin 1-like ELISA Kit; Netrin-1 ELISA Kit; Netrin1 ELISA Kit; Ntn 1 ELISA Kit; NTN 1L ELISA Kit; NTN1 ELISA Kit; NTN1L ELISA Kit; Unc6 ELISA Kit
Abbreviation NTN1
Uniprot No. O95631
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Ntn1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 82-94  
1:2 Average % 98  
Range % 94-101  
1:4 Average % 97  
Range % 90-104  
1:8 Average % 86  
Range % 82-91  
Recovery
The recovery of human Ntn1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-95  
EDTA plasma (n=4) 100 96-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.745 2.750 2.748 2.606  
1000 2.432 2.312 2.372 2.230  
500 1.833 1.827 1.830 1.688  
250 1.045 1.066 1.056 0.914  
125 0.658 0.703 0.681 0.539  
62.5 0.401 0.432 0.417 0.275  
31.25 0.266 0.275 0.271 0.129  
0 0.141 0.143 0.142    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews
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Sample type: Plasma (anticoagulant)

Sample species: Human

Sample dilution: No dilution

Review: I am using CSB-E11899h (human netrin-1 ELISA kit) for the first time. I have done various ELISA before, but the cusabio ELISA product has become my first choice product due to its sensitivity. I like to use this very much except there is an activation step for this netrin-1 protein. As soon as I activate the sample becomes coagulated. I haven\'t used any other Netrin-1 ELISA kit. So just facing this problem even in case of a fresh sample.

By Anonymous

Target Background

Function
(From Uniprot)
Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Involved in dorsal root ganglion axon projection towards the spinal cord. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.
Gene References into Functions
  1. High NTN1 expression is associated with gastric cancer. PMID: 30106432
  2. increased concentrations of urinary SEMA-3A and urinary Netrin-1 are found in urine from children with severe hydronephrosis and that their concentrations are related to the degree of obstruction. PMID: 29199452
  3. miR214 reduces chemoresistance by targeting netrin1 in bladder cancer cell lines. PMID: 29328435
  4. Netrin-1 decreased in experimental autoimmune encephalomyelitis and in multiple sclerosis patients, mainly during relapse, suggesting an anti-inflammatory role of netrin-1 PMID: 28677863
  5. Such data indicate that cooperation between the UPR and UNC5A depletion as previously observed by ourselves in HCC patients samples may foster liver cancer development and growth. PMID: 29277614
  6. Netrin-1 restores cell injury and impaired angiogenesis in vascular endothelial cells upon high glucose via PI3K/AKT-eNOS signaling. PMID: 28250059
  7. Collectively, our results defined netrin-1 as a positive regulator of malignant tumor metastasis in GC by activating the YAP signaling, with potential implications for new approaches to GC therapy. PMID: 29305865
  8. Results demonstrate that NTN1 is an important regulator of stemness and motility of glioblastoma cells. Furthermore, it deciphers a novel mechanism where NTN1 activates Notch signaling and subsequent stemness in invasive glioblastoma cells. PMID: 28069038
  9. Three mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. PMID: 28945198
  10. Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton's jelly mesenchymal stem cells. PMID: 28241866
  11. Our study has shown an increase in netrin-1 expression that could be linked with macrophage infiltration and polarization in the epicardial adipose tissue of patients with coronary artery disease PMID: 27727089
  12. netrin-1 degradation products are capable of modulating vascular permeability in diabetic retinopathy PMID: 27400127
  13. NETRIN1 signaling pathway is identified as a candidate pathway for major depressive disorder and should be explored in further large population studies. PMID: 27422368
  14. Enhanced detection of netrin-1-expressing CD14(low) cells in patients with systemic sclerosis-related interstitial lung disease was observed, and antibody-mediated netrin-1 neutralization attenuated detection of fibrocytes in all settings. PMID: 26749424
  15. we review the Netrin-1 mediated regulation of cancer, its potential use as a biomarker, and the targeting of the Netrin-1 pathway to treat cancers PMID: 28443497
  16. Our observations revealed that SOX6 is a tumor suppressor in ovarian cancer cells, and SOX6 exerts an inhibitory effect on the proliferation, invasion, and tumor cell-induced angiogenesis of ovarian cancer cells, whereas nerin-1 plays an opposite role and its expression is inversely correlated with SOX6. PMID: 28475012
  17. NTN1 rs9788972 is identified as a risk locus for nonsyndromic orofacial clefts susceptibility in a northern Chinese population; SNPs in PAX7 were not associated with any increased risk PMID: 28489749
  18. that netrin-1 may function as a positive regulator of hypoxia-triggered malignant behavior in prostate cancer by activating the Yes-associated protein signaling PMID: 27815019
  19. Data suggest that urinary netrin-1 may be a new biomarker for determining early tubular injury in obese children. PMID: 27087488
  20. cell proliferation and survival were decreased following targeted deletion of Netrin-1. Cell invasion was dramatically diminished in Netrin-1 knockdown Glioblastoma stem cells. Moreover, Netrin-1 knockdown Glioblastoma stem cells exhibited less proangiogenic activity. PMID: 27651158
  21. Data show that netrin-1 concentrations elevated in advanced non-small cell lung cancer compared to a healthy control group, and netrin-1 concentrations decreased with chemotherapy. PMID: 27067437
  22. upregulation of netrin-1 expression in different forms of cancer, and the increased expression of netrin-1 has been linked to its functions as a survival and invasion promoting factor. PMID: 27793362
  23. High Plasma netrin-1 levels are associated with peripheral artery disease in smokers. PMID: 26944719
  24. Netrin-1 reduced the Abeta1- 40-induced Abeta1-42 increase, increased sAbetaPPalpha, and reversed the Abeta-induced sAbetaPPalpha decrease in vitro PMID: 27060954
  25. Akt-FoxO1 signaling pathway plays an essential role during liver regeneration PMID: 26573873
  26. MUC4 has a role in promoting neural invasion mediated by the axon guidance factor Netrin-1 in pancreatic ductal adenocarcinoma PMID: 26393880
  27. Netrin-1 enhanced infectivity of hepatitis C virus particles and promoted viral entry by increasing the activation and decreasing the recycling of the epidermal growth factor receptor (EGFR), a protein that is dysregulated in hepatocellular carcinoma. PMID: 27031829
  28. NTN1-DCC pathway contains targets of FDA-approved drugs and may offer promise for guiding applied clinical research on preventive and therapeutic interventions for AMD PMID: 25950802
  29. These results identify Netrin-1 as a key regulator of osteoclast differentiation that may be a new target for bone therapies. PMID: 25483983
  30. Investigation of the mechanisms that trigger local translation revealed a role for calcium-dependent retrograde netrin-1/DCC receptor signaling. PMID: 25569157
  31. Netrin-1 stimulates phosphatase 1A to dephosphorylate YAP, which leads to decreased ubiquitination and degradation, enhancing YAP accumulation and signaling PMID: 26039999
  32. results demonstrate that netrin 1 is an important regulator of blood-brain barrier maintenance that protects the central nervous system against inflammatory conditions such as multiple sclerosis. PMID: 25903786
  33. Increased maternal netrin-4 levels in IUGR neonates may reflect in utero hypoxia, while the negative correlations between fetal netrin-1 and -4 levels may exert the dynamic balance between their angio- and anti-angiogenic properties. PMID: 24716747
  34. early urinary biomarker in the diagnosis of septic acute kidney injury PMID: 25154466
  35. Studies indicate that elevating netrin-1 expression increased migration and invasion in colorectal cancer cells. PMID: 24338005
  36. These studies demonstrate that semaphorin 3A and netrin-1 can be useful early diagnostic biomarkers of AKI after liver transplantation. PMID: 25289643
  37. Netrin-1 ameliorates myocardial infarction-induced myocardial injury. PMID: 24827071
  38. results of another in vitro assay, in which endothelial cells were co-cultured with human fibroblasts, however, showed that Mel2a-netrin1 CM inhibited tube formation through blocking elongation and coalescence of human umbilical vein endothelial cells PMID: 25022751
  39. Ntn-1 induces MMP-12-dependent E-cad degradation via the distinct activation of PKCalpha and FAK/Fyn, which is necessary to govern the activation of ERK, JNK, and NF-KB in promoting motility of umbilical cord blood-derived mesenchymal stem cells. PMID: 24738865
  40. Careful phenotyping of patients combined with molecular and functional studies in zebrafish identifed netrin-1 as a potential shared genetic factor for cardiac and thyroid congenital defects. PMID: 25353184
  41. netrin-1 promoted pancreatic cancer cell proliferation by upregulation of murine double minute 2 (Mdm2). PMID: 25001177
  42. Data indicate both total and nuclear netrin-1 expression as prognostic factors in brain metastases patients in contrast to other prognostic markers in oncology such as patient age, number of brain metastases or Ki67 proliferation index. PMID: 24647424
  43. In bladder cancer cells, netrin-1 expression is increased compared to control cells. PMID: 24528886
  44. promotes angiogenesis and accelerates atherosclerosis, protects the heart against ischemia-reperfusion injury, and reduces the infarct size. PMID: 24262644
  45. Netrin-1 promotes medulloblastoma cell invasiveness and angiogenesis, and demonstrates elevated expression in tumor tissue and urine of patients with pediatric medulloblastoma. PMID: 24812271
  46. Data indicate that dysregulation of expression of netrin-1 NTN1 in smooth muscle cell (SMC) and its chemorepulsive receptor UNC5B in macrophages are involved in the development of atherosclerosis and unstable plaques. PMID: 24122613
  47. These results suggest that in hypoxic HCC cells, netrin-1 activates downstream signaling pathways to induce EMT activation with subsequent production of multiple inflammatory mediators which in turn promotes cancer invasion. PMID: 24442237
  48. The netrin-1 upregulation which appears to be p53-dependent is a survival mechanism as netrin-1 silencing by siRNA is associated with a potentiation of cancer cell death upon Doxorubicin treatment. PMID: 24293316
  49. novel model for the spatial regulation of axon branching by Netrin-1, in which localized plasma membrane expansion occurs via TRIM9-dependent regulation of SNARE-mediated vesicle fusion. PMID: 24778312
  50. Netrin-1 is highly expressed in obese but not lean adipose tissue, where it directs the retention of macrophages, promoting inflammation. NTN1 also promotes insulin resistance. PMID: 24584118

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Subcellular Location Secreted. Cytoplasm.
Tissue Specificity Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level).
Database Links

HGNC: 8029

OMIM: 601614

KEGG: hsa:9423

STRING: 9606.ENSP00000173229

UniGene: Hs.660885

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