Human Semaphorin 3A(Sema 3A)ELISA Kit

Instructions
Code CSB-E15913h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Alternative Names SEMA3A ELISA Kit; SEMAD ELISA Kit; Semaphorin-3A ELISA Kit; Semaphorin III ELISA Kit; Sema III ELISA Kit
Abbreviation SEMA3A
Uniprot No. Q14563
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Sema 3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 96    
Range % 85-100    
1:2 Average % 87    
Range % 82-93    
1:4 Average % 98    
Range % 95-100    
1:8 Average % 92    
Range % 88-94    
Recovery
The recovery of human Sema 3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 92 88-96    
EDTA plasma (n=4) 97 90-104    
               
               
             
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
10 2.490 2.569 2.530 2.422    
5 2.171 2.196 2.184 2.076    
2.5 1.726 1.756 1.741 1.633    
1.25 1.284 1.306 1.295 1.187    
0.625 0.727 0.790 0.759 0.651    
0.312 0.461 0.481 0.471 0.363    
0.156 0.287 0.296 0.292 0.184    
0 0.107 0.109 0.108      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

May I have the following information regarding Human Semaphorin 3A, Sema 3A ELISA Kit (CSB-E15913h)
1)The active ingredient of the stop solution and its concentration ?
2)The type, concentration, and state (liquid/lyophilized) of the preservatives used ?
3) If the components are lyophilized, what is the concentration prior to reconstitution (i.e. mass of preservative used/ total mass of lyophilized vial contents)?
4)Expression host of the standards ?
5)Which components have H202 with the concentration of H202 in each, if any?
6) Are there any components that contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) in any of the components in this kit?
7) If yes, which components contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) and the concentration?

A:
Thanks for your inquiry.
CSB-E15913h
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
Standard is CHO derived recombinant protein. It is lyophilized powder and the concentration after reconstitution is 10ng/mL.
The kit doesn't contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) .
Pls let me know if you have any further questions. Thank you.

Target Data

Function Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
Gene References into Functions
  1. Semaphorin 3A is overexpressed in urothelial cancer patients, as evidenced both in its presence in urine and in bladder tissue. PMID: 29288007
  2. the inhibitory effect of Sema3C on microcapillary formation by human umbilical vein endothelial cells could be abrogated upon mutation at the Sema3C basic domain within putative furin cleavage site 742RNRR745, indicating that this site was essential for the Sema3 biological activity. PMID: 30304095
  3. Results provide evidence that SEMA3A is highly expressed in glioblastoma (GBM) compared to non-neoplastic brain tissues and SEMA3A signaling axis promotes GBM growth and invasion. PMID: 29129044
  4. these findings shows that miR-362 promotes lung cancer metastasis through downregulation of Sema3A PMID: 30155491
  5. These findings indicate a regulatory mechanism and a proapoptotic function of aberrant Sema3A signaling in Osteoarthritis (OA) chondrocytes, and suggest that targeting Sema3A signaling might interfere OA pathogenesis. PMID: 29111592
  6. increased concentrations of urinary SEMA-3A and urinary Netrin-1 are found in urine from children with severe hydronephrosis and that their concentrations are related to the degree of obstruction. PMID: 29199452
  7. Advanced peri-implantitis lesions showed higher levels of gene expression for Sem3A and Sem4D and lower levels of Sem4A in comparison to tissues obtained from a healthy dental implant. PMID: 29763494
  8. clinical samples from apical periodontitis patients were obtained to analyse the expression of Sema3A/Nrp1. These results indicated that the bone destruction level expanded from days 7 to 35 PMID: 29457037
  9. study demonstrates that Sema3E/plexinD1 inhibits proliferation and migration of vascular smooth muscle cells via inactivation of Rap1-AKT signalling pathways PMID: 29016743
  10. Results showed a preferential association of NRP1 with SEMA3A, suggesting that SEMA3A can partially reverse the effects caused by the VEGFA preventing its binding with the NRP1 receptor. PMID: 28636974
  11. Our observation of a second case supports the notion that bi-allelic mutations in SEMA3A cause an autosomal recessive type of syndromic short stature. PMID: 28075028
  12. the upregulation of SEMA3A in hepatocellular carcinoma (HCC)cells promoted tumor growth and progression in an HCC mouse model. These results indicate that SEMA3A enhances CapG, galectin-3, enolase 2 and EpCAM expression to promote HCC progression and is a potential therapeutic target for HCC. PMID: 28498470
  13. Sema3A as a novel regulator of airway smooth cell proliferation by suppressing Rac1, GSK-3beta and STAT3 signaling mediated by Nrp1. PMID: 27791986
  14. provide an easy and reproducible method to analyze growth cone sensitivity to the prototypic guidance molecule family class 3 semaphorin PMID: 27787850
  15. Measuring electrical impedance allows real-time monitoring of changes in endothelial cell morphology and adhesion induced by SEMA3E via plexin D1 PMID: 27787852
  16. describe a method where in vitro cultures of growth cones are incubated with these ligands in the presence or absence of Sema3A, followed by stripping of remaining ligand on cell-surface and analysis by immunofluorescence techniques PMID: 27787860
  17. Semaphorin 3A increased tumor-infiltrating macrophages (TAM) infiltration and promoted hepatocellular carcinoma (HCC) progression. Semaphorin 3A expression alone, or combined with the number of TAMs, is a new prognostic factor and potential target for the treatment of HCC. PMID: 27351132
  18. Significant genetic risk for Hirschsprung disease (HSCR) was imparted by rs2435357 and rs2506030 at RET and by rs12707682 at SEMA3 in a Chinese population. No evidence was found of a genetic association between HSCR and either of the NRG1 SNPs rs7835688 and rs16879552, at either allele or genotype level. PMID: 27203398
  19. rs139438618 at the semaphorin 3A locus was significantly associated with Alcohol Dependence and Major Depression comorbidity in African Americans. There was no significant association identified in European American participants. PMID: 29071344
  20. Serum level of Sema3A taken from diagnosed celiac without gluten-free diet was higher significantly than healthy controls. PMID: 28502679
  21. Sema3A serum levels and the expression of sema3A on Tregs was significantly lower in patients with moderate to severe asthma when compared to healthy individuals. PMID: 28502680
  22. Sema3a-Nrp2 signaling along an unrecognized pancreatic developmental axis constitutes a chemoattractant system essential for generating the hallmark morphogenetic properties of pancreatic islets. PMID: 28893946
  23. EV-delivered Sema3A orchestrates loss of barrier integrity in glioblastoma PMID: 26364614
  24. IL6 underexpression is associated with Breast Cancer. PMID: 27197153
  25. We also demonstrate that the effects of RET and NRG1 are universal across European and Asian ancestries. In contrast, we detected a European-specific association of a low-frequency variant, rs80227144, in SEMA3. Conditional analyses on the lead SNPs revealed a secondary association signal, corresponding to an Asian-specific, low-frequency missense variant encoding RET p.Asp489Asn PMID: 27702942
  26. Semaphorin3A serum concentrations were similar in ankylosing spondylitis patients and controls and were not altered on treatment in anti-TNF agents. PMID: 27782869
  27. The self-renewal ability abolished by Sema3a knockdown could not be recovered by exogenous addition of recombinant SEMA3A. In addition, the activity of mammalian target of rapamycin complex 1 (mTORC1) and the expression of its substrate p70S6K1 were also decreased. These results demonstrate that Sema3a is a potential therapeutic target in eradication of CSCs. PMID: 27769863
  28. Study shows a significant reduction of the serum Sema3A level and its mRNA expression in peripheral blood mononuclear cells in patients with relapsing-remitting multiple sclerosis compared with normal individuals. PMID: 28188869
  29. Genetic analyses of susceptibility alleles at the SEMA3 locus in families of Caucasian ancestry show that a significant genetic risk can be detected at SEMA3 rs11766001, rs12707682, and rs1583147 variants. PMID: 27469503
  30. Sema3A expression on T and B regulatory lymphocytes is low in familial Mediterranean fever patients during attack and in smoldering disease compared to the expression in remission and healthy controls. PMID: 27157528
  31. Tumor cells decrease Sema3a mRNA expression in osteoblasts in an mTORC1-dependent manner. PMID: 26865270
  32. Semaphorin-3a, neuropilin-1 and plexin-A1 are axonal guidance molecules that have been recently implicated in regulating bone metabolism. PMID: 26602825
  33. Sema3A and sema7A expression correlated with the inflammatory activity of the multiple sclerosis (MS) lesions, suggesting their involvement in the immunological process that takes place in MS. PMID: 26432853
  34. Sema3A expression is low in systemic sclerosis serum and more specifically on regulatory T cells. PMID: 25895648
  35. Findings show the involvement of amphiregulin and semaphorin-3A in the improvement of skin innervations and penetration of nerve fibers into the epidermis. PMID: 26201903
  36. LL-37 induces SeMA3A expression in epidermal keratinocytes. Possible therapy for pruritus in atopic dermatitis. PMID: 26121211
  37. Vital roles for semaphorin signaling in vascular patterning and cardiac morphogenesis have been demonstrated, and impaired semaphorin signaling has been associated with various human cardiovascular disorders. PMID: 25651171
  38. our data suggest that MCF-7 cell-derived Sema 3A plays a causative role in osteoblastic bone metastases progression by stimulating osteoblastic differentiation. PMID: 25973043
  39. Increased urinary SEMA3A is independently associated with CKD in both diabetic and non diabetic hypertensive patients. PMID: 24756974
  40. Despite limited research in this field, our aim is to promote further understanding of the function of Sema3A in the skeletal system. PMID: 25955818
  41. Genetic risk for Hirschsprung disease in individuals of European ancestry can only be detected at RET (rs2435357 and rs2506030) and at SEMA3 (rs11766001), but not at NRG1. PMID: 25666438
  42. Serum semaphoring 3A levels are reduced after hemodialysis. PMID: 25560370
  43. Decreased semaphorin 3A expression is associated with a poor prognosis in patients with epithelial ovarian carcinoma PMID: 25812535
  44. although plexin-A4 overexpression restored Sema3A signaling in plexin-A1-silenced cells, it failed to restore Sema3B signaling in plexin-A2-silenced cells. PMID: 25335892
  45. the general effects of Sema3A on bone metabolism are weak and not clear as evidenced by lack of association between this parameter and bone mineral densities and osteoporotic fractures PMID: 25050899
  46. Semaphorin-3A involvement in autoimmune responses, diabetic nephropathy and diabetic retinopathy is discussed. PMID: 25331060
  47. These findings indicate that excess sema3a promotes severe diabetic nephropathy and identifies novel potential therapeutic targets for diabetic nephropathy. PMID: 25475434
  48. Data proposed a novel signaling mechanism by which Sema 3A regulates PTEN, FOXO 3a and MelCAM in a coordinated manner that leads to suppression of breast cancer growth and angiogenesis. PMID: 24727891
  49. These studies demonstrate that semaphorin 3A and netrin-1 can be useful early diagnostic biomarkers of AKI after liver transplantation. PMID: 25289643
  50. These results suggest that Sema3A may possess the function to convert periodontal ligament cells into mesenchymal-stem-like cells. PMID: 24380401
  51. HMGB1 promotes tumor cell migration through epigenetic silencing of semaphorin 3A. PMID: 24213571
  52. Our results suggest that prostate cancer-induced osteoblastic differentiation is at least partially mediated by Sema 3A, and may be regulated by the beta-catenin signalling pathway. PMID: 25421946
  53. Demonstrate SEMA3A as a naturally occurring protein that selectively inhibits Kv4.3 and SEMA3A as a possible Brugada syndrome susceptibility gene through a Kv4.3 gain-of-function mechanism. PMID: 24963029
  54. SEMA3a enhanced CD72 expression in B cells of both healthy controls and SLE patients. PMID: 24461079
  55. heterozygous missense variants in SEMA3A and SEMA7A may modify the phenotype of Kallmann syndrome but most likely are not alone sufficient to cause the disorder PMID: 24522099
  56. Sema3A is a new player in the regulation of bone remodeling. [Review] PMID: 24589620
  57. Stimulation of nonmodified endothelial cells with Sema3A resulted in an up regulation of CIITA expression, which was associated with enhanced HLA-DR levels and an increase in alloreactive CD4+ T-cell proliferation. PMID: 24673430
  58. Biallelic SEMA3A defects cause a novel type of syndromic short stature. PMID: 24124006
  59. sema3A plays a role in the pathogenesis of allergic rhinitis and atopic dermatitis. [review] PMID: 24713604
  60. SEMA3A expression positively correlates with itching sensation and number of nerve fibers in the epidermis in prurigo nodularis PMID: 23398122
  61. Loss of Sema3A expression is associated with biochemical recurrence of patients with low- and intermediate-risk prostate cancer. PMID: 23906303
  62. in this study we have analyzed three aminoacidic substitutions, A131T-SEMA3A, S598G-SEMA3A and E198K-SEMA3D. These variants result in an increase of SEMA proteins levels in the HSCR colon tissue. PMID: 23372769
  63. A high incidence of SEMA3A(I334V) in UCA patients and inappropriate innervation patterning in their hearts implicate involvement of the SEMA3A gene in the pathogenesis of UCA. PMID: 23593010
  64. Reduction of Sema3A expression in rheumatoid arthritis synovial tissues may contribute to pathogenesis of RA. PMID: 23343469
  65. This is the first study in which a reduced serum level of sema3A was found in association with systemic lupus erythematosus disease activity PMID: 22697500
  66. Semaphorin 3A impinged upon the basal activity of the serine phosphatase PP2A and disrupted PP2A interaction with VE-cadherin, leading to cell-cell junction disorganization and increased permeability. PMID: 22685328
  67. Decreased skin Sema3A expression may play a role in pruritus and the pathogenesis of psoriasis. PMID: 22565412
  68. This study demonstrated a marked loss of noradrenergic and sensory nerve fibers in polyp mucosa, which was associated with a strong increase of semaphorin 3F and 3A. PMID: 22093159
  69. sema3A is reduced in systemic lupus erythematosus (SLE) patients in correlation with disease severity and autoimmunity; memory B cells are beneficially responsive to sema3A PMID: 22875653
  70. Semaphorin 3A has a role in the development of the olfactory system and in neuronal control of puberty in humans. PMID: 22416012
  71. Sema3A is overexpressed, with a direct correlation with cloning, in osteoarthritic cartilage and that it suppresses the VEGF165-promoted migration of chondrocytes. PMID: 21953086
  72. A review of how neuron-derived factors, GPR91 and Semaphorin 3A, guide retinal vascularization and are major contributors to the pathogenesis of retinopathy of prematurity . PMID: 22497252
  73. Findings indicate that semaphorin-3A signaling insufficiency contributes to the pathogenesis of Kallmann syndrome. Nonsynonymous mutations in SEMA3A were found in 24 patients, all in heterozygous state . PMID: 22927827
  74. Our results for rs7804122 polymorphism provid preliminary evidence that the SEMA3A gene is involved in the susceptibility to Hirschsprung disease in the Northeastern Chinese population. PMID: 22184102
  75. SEMA-3A is involved in the regulation of CXCL12-driven human thymocyte migration, where it acts as a physiological antagonist. PMID: 21878545
  76. the downregulated expression of SEMA3A and several SEMA3s results in a loss of inhibitory activities in tumor angiogenesis and tumor growth of VEGFA. PMID: 21842119
  77. A decrease in class 3 semaphorin and their plexin receptors may have some relationship with disease progression in ductal breast carcinoma. PMID: 21884206
  78. SEMA3A expression was upregulated in the aganglionic smooth muscle layer of the colon in some patients with HSCR and our data suggest that increased SEMA3A expression may be a risk factor for HSCR pathology PMID: 21656899
  79. We found no genome-wide statistically significant associations but identified several plausible candidate genes among findings at p < 5E-05: SEMA3A, TMEM132D, LRRC7, ALK, and STIP1. PMID: 21784300
  80. Both systemic and tumor-targeted delivery of SEMA3A inhibits tumor angiogenesis and tumor growth in multiple mouse models; moreover, SEMA3A inhibits the metastatic spreading from primary tumors. PMID: 21205984
  81. incubation of breast tumor cells with recombinant Sema3A rapidly increased eIF4E activity, RhoA protein levels, and RhoA activity; Sema3A impedes breast tumor cell migration in part by stimulating RhoA PMID: 20655307
  82. Results demonstrate the critical role of Galectin-1 and Sema-3A in mesenchymal stem cell functions and may open new perspectives in the understanding and treatment of various immune and neoplastic disorders. PMID: 19886821
  83. In degenerate intervertebral disc samples, sema3A expression decreased significantly PMID: 20051117
  84. these findings indicate that semaphorin 3A released from corneal fibroblasts may play an important role in the regulation of intercellular communication between corneal epithelial cells as well as in the maintenance of corneal structure and function. PMID: 20382125
  85. neither Sema3A nor Sema4D likely influence the susceptibility to Alzheimer's disease PMID: 19957197
  86. NRP2 is necessary to trigger Sema3A-induced glioma cell repulsion and attraction. PMID: 19855168
  87. Dysregulation of the Sema3A pathway plays a key role in prostatic cancer progression. PMID: 19817889
  88. SEMA3A suppression of tumor cell migration is dependent on alpha2beta1, the expression of which is stimulated in breast tumor cells by an autocrine SEMA3A pathway. PMID: 18787945
  89. sema3A is elevated in schizophrenia, and is associated with downregulation of genes involved in synaptic formation and maintenance. PMID: 12610647
  90. human glioma cells express class 3 semaphorins and receptors for soluble and membrane-bound semaphorins, suggesting a possible role of the semaphorin/neuropilin system in the interactions of human malignant glioma with the nervous and immune systems. PMID: 12730958
  91. during vascular development and experimental angiogenesis, endothelial cells generate autocrine chemorepulsive signals of class 3 semaphorins (SEMA3 proteins) that localize at nascent adhesive sites in spreading endothelial cells PMID: 12879061
  92. Breast carcinoma cells support an autocrine pathway involving SEMA3A, plexin-A1, and NP1 that impedes their ability to chemotax. PMID: 14500350
  93. new role of Sema-3A in VEGF function mediated by p38 MAPK and suggest that the abrogation of regulated Sema-3A expression is responsible for VEGF-driven growth of tumor cells PMID: 14656993
  94. the chemorepulsive signals mediated by Sema 3A play an important role in preventing nerve fibers growth in the umbilical cord and in gestational uterine tissues. PMID: 15517571
  95. extensive inhibition of platelet function by Sema3A appears to be mediated, at least in part, through impairment of agonist-induced Rac1-dependent actin rearrangement PMID: 15831706
  96. Down-regulation of NRP1 by NRSF overexpression reduced Sema3A activity. It was concluded that NRSF is a transcription factor that silences NRP1 expression and thereby diminishes the Sema3A mediated inhibition of HaCaT keratinocyte migration PMID: 16330548
  97. Vascular endothelial growth factor165 (VEGF165) and semaphorin3A (SEMA3A) elicit pro- and antiangiogenic signals respectively in endothelial cells (ECs) by binding to their receptors VEGFR-2, neuropilin-1 and plexin-a. PMID: 16684957
  98. In the regulation of the immune response Sema-3A plays a novel role as a modulator of cross-talk between activated dendritic cells and T cells. PMID: 16791896
  99. NP-1/Sema-3A-mediated interactions participate in the control of human thymocyte development PMID: 17369353
  100. sema3A and sema3C have opposite roles in neoplasm invasiveness and adhesion PMID: 17390026

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Involvement in disease Hypogonadotropic hypogonadism 16 with or without anosmia (HH16)
Subcellular Location Secreted
Protein Families Semaphorin family
Database Links

HGNC: 10723

OMIM: 603961

KEGG: hsa:10371

STRING: 9606.ENSP00000265362

UniGene: Hs.252451

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