Human Serglycin(SRGN) ELISA kit

Code CSB-EL022664HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
serglycin
Alternative Names
Chondroitin sulfate proteoglycan core protein ELISA Kit; Cytolytic granule proteoglycan core protein ELISA Kit; FLJ12930 ELISA Kit; gp600 ELISA Kit; Hematopoetic proteoglycan core protein ELISA Kit; Mastocytoma proteoglycan core protein ELISA Kit; MGC9289 ELISA Kit; OTTHUMP00000019716 ELISA Kit; P.PG ELISA Kit; PG19 core protein ELISA Kit; Pgsg ELISA Kit; Platelet proteoglycan core protein ELISA Kit; platelet proteoglycan protein core ELISA Kit; PLATELET PROTEOGLYCAN PROTEIN CORE; PPG ELISA Kit; PPG ELISA Kit; PRG ELISA Kit; PRG1 ELISA Kit; PROTEOGLYCAN 1 ELISA Kit; proteoglycan 1; secretory granule ELISA Kit; Proteoglycan 10K core protein ELISA Kit; Proteoglycan peptide core protein ELISA Kit; PROTEOGLYCAN PROTEIN CORE FOR MAST CELL SECRETORY GRANULE ELISA Kit; secretory granule proteoglycan 1 ELISA Kit; Secretory granule proteoglycan core peptide ELISA Kit; Secretory granule proteoglycan core protein ELISA Kit; Serglycin ELISA Kit; serglycin proteoglycan ELISA Kit; Sgc ELISA Kit; Srgn ELISA Kit; SRGN_HUMAN ELISA Kit
Abbreviation
SRGN
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SRGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 107    
Range % 103-112    
1:2 Average % 97    
Range % 93-102    
1:4 Average % 94    
Range % 88-98    
1:8 Average % 96    
Range % 92-99    
Recovery
The recovery of human SRGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 93 88-97    
EDTA plasma (n=4) 87 82-91    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
20 2.612 2.535 2.574 2.414    
10 2.265 2.171 2.218 2.058    
5 1.667 1.632 1.650 1.490    
2.5 1.078 1.064 1.071 0.911    
1.25 0.675 0.669 0.672 0.512    
0.625 0.443 0.438 0.441 0.281    
0.312 0.328 0.323 0.326 0.166    
0 0.161 0.159 0.160      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human SRGN ELISA Kit was designed for the quantitative measurement of Human SRGN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization.
Gene References into Functions
  1. SRGN overexpression promoted colorectal cancer cell migration and invasion and SRGN was physically binding to a hypoxia response element in its promoter region. PMID: 30121667
  2. SRGN secreted by tumor cells and stromal components in the TME promotes malignant phenotypes through interacting with tumor cell receptor CD44 PMID: 27819672
  3. Extracellular spce serglycin upregulated CD44 receptor expression to maintain nasopharyngeal carcinoma stemness by interacting with CD44 and activating the MAPK/beta-catenin pathway. PMID: 27809309
  4. SRGN expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  5. Patients with NPC with tumors showing strong tumor intensity and low infiltrated percentage of tumor-infiltrated lymphocytes with serglycin may be at high risk for distant metastases. PMID: 24995621
  6. The elevated levels of serglycin in aggressive cancer and stromal cells may suggest a key role for serglycin in disease progression PMID: 26581653
  7. These results suggest functions for serglycin in endothelial cells trough interactions with partner molecules, in biological processes with relevance for diabetic complications, cardiovascular disease and cancer development. PMID: 26694746
  8. These results suggest that different signaling pathways are involved in regulating secretion of serglycin and partner molecules in activated endothelial cells. PMID: 24513305
  9. The reduced expression of SRGN was accompanied by elevated levels of E-cadherin. PMID: 23996242
  10. These studies provide direct evidence for a critical role for serglycin in MM pathogenesis and show that targeting serglycin may provide a novel therapeutic approach for MM. PMID: 24403068
  11. These results suggest that human epicardial adipose tissue (EAT) has strong inflammatory properties in patients with coronary artery disease and provide novel evidence that serglycin is an adipocytokine highly expressed in EAT. PMID: 23376071
  12. Cell-surface SRGN promotes the adhesion of myeloma cells to collagen type I. PMID: 23387827
  13. This review covers recent studies on the involvement of serglycin in various pathological conditions, as well as its roles in immunity, hemostasis, cell growth, apoptosis, and reproduction. PMID: 22807344
  14. Human granulocyte precursors transfected with siRNA against serglycin displayed reduced capability to retain fully processed HNP-1. PMID: 21849484
  15. Serglycin regulates NPC metastasis via autocrine and paracrine routes, and that it serves as an independent prognostic indicator of metastasis-free survival and disease-free survival in NPC patients. PMID: 21289131
  16. Serglycin secreted by human multiple myeloma cell lines inhibits both the classical and lectin pathways of complement, without influencing alternative pathway activity. PMID: 21268013
  17. Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1. PMID: 21075844
  18. proapoptotic granzyme is exocytosed predominantly as a macromolecular complex with serglycin PMID: 12388539
  19. cell- and differentiation-specific alterations in chromatin structure may control serglycin gene expression PMID: 14506241
  20. serglycin-bound granzyme B in high-molecular-weight degranulate material from cytotoxic T lymphocytes predominantly followed a dynamin-dependent pathway to kill target cells PMID: 14739229
  21. mechanism of delivery is proposed entailing electrostatic transfer of granzyme B from serglycin to cell surface proteins PMID: 15788411
  22. Serglycin release is a constitutive process, which may be of fundamental biological importance in the study of multiple myeloma. PMID: 16870619
  23. Serglycin is of importance for secretory processes in human monocytes. PMID: 17909965
  24. Mutations in SRGN is not associated with familial hemophagocytic lymphohistiocytosis PMID: 18000860

Show More

Hide All

Subcellular Location
Cytoplasmic granule. Cytolytic granule. Secreted, extracellular space. Golgi apparatus.
Protein Families
Serglycin family
Database Links

HGNC: 9361

OMIM: 177040

KEGG: hsa:5552

STRING: 9606.ENSP00000242465

UniGene: Hs.1908

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1