Human Sialic acid-binding Ig-like lectin 8(SIGLEC8) ELISA kit

Instructions
Code CSB-EL021302HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name sialic acid binding Ig-like lectin 8
Alternative Names CD329 antigen ELISA Kit; CDw329 ELISA Kit; SAF 2 ELISA Kit; SAF-2 ELISA Kit; SAF2 ELISA Kit; Sialic acid binding Ig like lectin 8 ELISA Kit; Sialic acid-binding Ig-like lectin 8 ELISA Kit; Sialoadhesin family member 2 ELISA Kit; SIGL8_HUMAN ELISA Kit; Siglec 8 ELISA Kit; Siglec-8 ELISA Kit; SIGLEC8 ELISA Kit; SIGLEC8L ELISA Kit
Abbreviation SIGLEC8
Uniprot No. Q9NYZ4
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SIGLEC8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 100
Range % 95-107
1:2 Average % 96
Range % 92-100
1:4 Average % 84
Range % 80-92
1:8 Average % 88
Range % 84-92
Recovery
The recovery of human SIGLEC8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 90-100
EDTA plasma (n=4) 90 85-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.164 2.246 2.205 2.040
5 1.618 1.634 1.626 1.461
2.5 1.201 1.175 1.188 1.023
1.25 0.792 0.823 0.808 0.643
0.625 0.558 0.571 0.565 0.400
0.312 0.419 0.442 0.431 0.266
0.156 0.324 0.305 0.315 0.150
0 0.162 0.167 0.165  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.
Gene References into Functions
  1. In differentiating ,eosinophils, SIGLEC-8 messenger RNA and protein were markedly down-regulated in parallel with OLIG2 by an OLIG2 small interfering RNA or a short hairpin RNA. An E-box in the first intron was found to stimulate SIGLEC-8 gene transcription and to bind OLIG2. PMID: 27154355
  2. miR-215 acts in concert with the host gene IARS2 to affect neuron migration and proliferation through the target gene SIGLEC-8. PMID: 28006787
  3. Our study suggested that intratumoral Siglec-8 expression was an independent prognostic factor for overall survival of patients with gastric cancer PMID: 26883254
  4. Siglec-8 expression is sensitive to tiotropium and formoterol, indicating that it may be involved in COPD pathogenesis and may influence COPD phenotyping PMID: 23835953
  5. In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
  6. Engagement of Siglec-8 on blood eosinophils results in caspase- and mitochondria-dependent apoptosis. PMID: 22324980
  7. Report the development of a sensitive competitive ELISA to detect sSiglec-8 in human serum samples obtained from patients with various forms of eosinophilia. PMID: 22683541
  8. Eosinophils primed by IL-33 and/or IL-5 in vivo would be expected to display enhanced susceptibility to undergoing Siglec-8-induced apoptosis. PMID: 22079334
  9. expressed on eosinophils and basophils from subjects with chronic eosinophilic leukemia, chronic myelogenous leukemia, and on malignant and non-malignant bone marrow mast cells PMID: 21938510
  10. The Siglec-8 gene may be a susceptibility locus for asthma. PMID: 20087405
  11. Sialic acid binding immunoglobulin-like lectin 8 (Siglec-8) cross-linking with antibodies rapidly generated caspase-3-like activity and reduced eosinophil viability through induction of apoptosis PMID: 12609831
  12. Siglec-8 binds preferentially to a sLex structure bearing an additional sulfate ester on the galactose 6-hydroxyl PMID: 15563466
  13. Siglec-8-induced apoptosis in eosinophils occurs through the sequential production of reactive oxygen species, followed by induction of mitochondrial injury and caspase cleavage. PMID: 16157303
  14. IL-5 priming enhances Siglec-8-mediated mitochondrial and ROS-dependent eosinophil apoptosis and eliminates caspase dependence. PMID: 17690326
  15. First reported inhibitory effects of Siglec engagement on human mast cells. PMID: 18036650
  16. A review of the cytotoxic effects of natural anti-Siglec8 autoantibodies on both neutrophils and eosinophils. PMID: 18558361
  17. REVIEW: Siglec-8 and Siglec-F function on human eosinophils, and Siglec-8 function on mast cells PMID: 19178537
  18. Selective and specific engagement and binding of Siglec-8 on eosinophils by a synthetic 6'-sulfo-sLe(x)-containing ligand could be useful for controlling eosinophilic inflammatory responses in vivo. PMID: 19458105

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Subcellular Location Membrane; Single-pass type I membrane protein.
Protein Families Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Tissue Specificity Expressed specifically on red blood cells namely basophil, mast cells and eosinophils.
Database Links

HGNC: 10877

OMIM: 605639

KEGG: hsa:27181

STRING: 9606.ENSP00000321077

UniGene: Hs.447899

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