SIGLEC8 Antibody

Code CSB-PA005297
Size US$100
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  • Western Blot analysis of K562 cells using CD329 Polyclonal Antibody
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Product Details

Uniprot No.
Target Names
SIGLEC8
Alternative Names
CD329 antigen antibody; CDw329 antibody; SAF 2 antibody; SAF-2 antibody; SAF2 antibody; Sialic acid binding Ig like lectin 8 antibody; Sialic acid-binding Ig-like lectin 8 antibody; Sialoadhesin family member 2 antibody; SIGL8_HUMAN antibody; Siglec 8 antibody; Siglec-8 antibody; SIGLEC8 antibody; SIGLEC8L antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Synthesized peptide derived from the Internal region of Human CD329.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:20000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.
Gene References into Functions
  1. In differentiating ,eosinophils, SIGLEC-8 messenger RNA and protein were markedly down-regulated in parallel with OLIG2 by an OLIG2 small interfering RNA or a short hairpin RNA. An E-box in the first intron was found to stimulate SIGLEC-8 gene transcription and to bind OLIG2. PMID: 27154355
  2. miR-215 acts in concert with the host gene IARS2 to affect neuron migration and proliferation through the target gene SIGLEC-8. PMID: 28006787
  3. Our study suggested that intratumoral Siglec-8 expression was an independent prognostic factor for overall survival of patients with gastric cancer PMID: 26883254
  4. Siglec-8 expression is sensitive to tiotropium and formoterol, indicating that it may be involved in COPD pathogenesis and may influence COPD phenotyping PMID: 23835953
  5. In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
  6. Engagement of Siglec-8 on blood eosinophils results in caspase- and mitochondria-dependent apoptosis. PMID: 22324980
  7. Report the development of a sensitive competitive ELISA to detect sSiglec-8 in human serum samples obtained from patients with various forms of eosinophilia. PMID: 22683541
  8. Eosinophils primed by IL-33 and/or IL-5 in vivo would be expected to display enhanced susceptibility to undergoing Siglec-8-induced apoptosis. PMID: 22079334
  9. expressed on eosinophils and basophils from subjects with chronic eosinophilic leukemia, chronic myelogenous leukemia, and on malignant and non-malignant bone marrow mast cells PMID: 21938510
  10. The Siglec-8 gene may be a susceptibility locus for asthma. PMID: 20087405
  11. Sialic acid binding immunoglobulin-like lectin 8 (Siglec-8) cross-linking with antibodies rapidly generated caspase-3-like activity and reduced eosinophil viability through induction of apoptosis PMID: 12609831
  12. Siglec-8 binds preferentially to a sLex structure bearing an additional sulfate ester on the galactose 6-hydroxyl PMID: 15563466
  13. Siglec-8-induced apoptosis in eosinophils occurs through the sequential production of reactive oxygen species, followed by induction of mitochondrial injury and caspase cleavage. PMID: 16157303
  14. IL-5 priming enhances Siglec-8-mediated mitochondrial and ROS-dependent eosinophil apoptosis and eliminates caspase dependence. PMID: 17690326
  15. First reported inhibitory effects of Siglec engagement on human mast cells. PMID: 18036650
  16. A review of the cytotoxic effects of natural anti-Siglec8 autoantibodies on both neutrophils and eosinophils. PMID: 18558361
  17. REVIEW: Siglec-8 and Siglec-F function on human eosinophils, and Siglec-8 function on mast cells PMID: 19178537
  18. Selective and specific engagement and binding of Siglec-8 on eosinophils by a synthetic 6'-sulfo-sLe(x)-containing ligand could be useful for controlling eosinophilic inflammatory responses in vivo. PMID: 19458105

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Tissue Specificity
Expressed specifically on red blood cells namely basophil, mast cells and eosinophils.
Database Links

HGNC: 10877

OMIM: 605639

KEGG: hsa:27181

STRING: 9606.ENSP00000321077

UniGene: Hs.447899

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