Human Triggering Receptor Expresses on Myeloid Cells-1,TREM-1 ELISA Kit

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Code CSB-E04836h
Size 96T,5×96T,10×96T
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Product Details

Target Name triggering receptor expressed on myeloid cells 1
Alternative Names CD354 ELISA Kit; OTTMUSP00000018206 ELISA Kit; TREM 1 ELISA Kit; TREM-1 ELISA Kit; TREM1 ELISA Kit; TREM1_HUMAN ELISA Kit; Triggering receptor expressed on monocytes 1 ELISA Kit; Triggering receptor expressed on myeloid cells 1 ELISA Kit; Triggering receptor TREM 1 ELISA Kit; Triggering receptor TREM1 ELISA Kit
Abbreviation TREM1
Uniprot No. Q9NP99
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %97
Range %92-102
1:2Average %90
Range %82-97
1:4Average %104
Range %99-110
1:8Average %86
Range %82-93
Recovery
The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9084-96
EDTA plasma (n=4)9690-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.529 2.752 2.641 2.538
10002.038 2.079 2.059 1.956
5001.304 1.389 1.347 1.244
2500.701 0.733 0.717 0.614
1250.441 0.455 0.448 0.345
62.50.302 0.326 0.314 0.211
31.250.205 0.201 0.203 0.100
00.104 0.101 0.103  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.
Gene References into Functions
  1. These data show that human macrophages infected with human immunodeficiency virus-1 show an increased expression of TREM-1. In parallel, direct exposure to the human immunodeficiency virus-1-related proteins Tat or gp120 induces TREM-1 expression in macrophages and confers anti-apoptotic attributes. PMID: 28181540
  2. Current data suggest that sTREM-1 might become a useful biomarker for the prediction of neonatal sepsis; however, the significant variation in the used threshold values (that ranged between 77.5 and 1707.35 pg/ml) and the methodological differences across studies limit the value of our findings in clinical practice.[Review] PMID: 29644420
  3. TREM-1 is a predictive biomarker of in-stent restenosis and an important mediator of cellular inflammation, migration, and proliferation in vascular smooth muscle cells PMID: 29080545
  4. The present study highlights the role of urine sTREM-1 levels in late-onset sepsis. PMID: 29480083
  5. TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis. PMID: 27762264
  6. serum levels seem higher in newly diagnosed type 1 diabetes patients than in their healthy siblings (not statistically significant) PMID: 27862781
  7. TREM-1 interventions by means of LP17, LR12 and TREM-1 fusion protein did not ameliorate IR-induced injury. In the human renal transplant cohort, donor and recipient TREM1 gene variant p.Thr25Ser was not associated with DGF, nor with biopsy-proven rejection or death-censored graft failure. We conclude that TREM-1 does not play a major role during experimental renal IR and after kidney transplantation PMID: 27928159
  8. TREM-1 plays an important role in mediating platelet activation. PMID: 28837205
  9. Serum sTREM-1 level is significantly higher in diarrhea-predominant irritable bowel syndrome (D-IBS) patients and positively correlates with abdominal pain, which may be initiated by TREM-1-associated macrophage activation, indicating the existence of subclinical inflammation in D-IBS. PMID: 29516326
  10. TREM-1 overexpression and M1 macrophage polarization are associated with obesity-induced insulin resistance. PMID: 28454543
  11. TREM-1 may have a role in pathology of craniopharyngioma and Rathke's cleft cyst PMID: 27409178
  12. low expression level of TREM-1 might be a characteristic for tumor-associated macrophages in lung cancer PMID: 27244892
  13. The results demonstrate that both commensal and pathogenic oral bacteria activate the TREM-1 pathway, resulting in a proinflammatory TREM-1 activity-dependent increase in proinflammatory cytokine production. PMID: 27448788
  14. Our findings suggest a novel modulatory role of TREM-1 in the pathogenesis of SLE. sTREM-1 production is a useful diagnostic marker and a molecular target for combination therapy of lupus. PMID: 28089248
  15. In the premature rupture of membranes group,the NF-kappaB p65 and sTREM-1 levels in maternal blood were significantly higher in women with chorioamnionitis than women without chorioamnionitis (P<.05). PMID: 27521929
  16. Diagnostic value of sTREM-1, IL-8, PCT, and CRP in febrile neutropenia after autologous stem cell transplantation PMID: 28920169
  17. findings showed detectable expression of TREM-1 in 100% of samples, urinary tract infection (UTI) and non-UTI groups; levels of sTREM-1 were detected in patients with UTI, but not in non-UTI patients; TREM-1 levels in patients with UTI tend to be higher and are associated with increased neutrophils and cytokine activity induced by bacteria PMID: 28367708
  18. The minor allele T of rs2234246 of TERM1 protein, was associated with increased sTREM-1 in the discovery population, explaining 33% of its variance, and with increased levels of mRNA. PMID: 28771614
  19. This study provided evidence that rs6910730G, an intronic variant of TREM1, reduced the ability of human monocytes for Abeta phagocytosis, and this reduction was likely attributed to a decreased monocytic TREM1 expression. PMID: 27670763
  20. Studies show that bronchoalveolar lavage fluid (BALF) soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) levels do not discriminate between mild-to-moderate asthma and chronic obstructive pulmonary disease (COPD), in contrast, the interleukin 6 (IL-6)/interleukine 13 (IL-13) ratio measured in BALF effectively differentiated these two diseases. PMID: 28791839
  21. study found that soluble TREM-1 is increased and correlates with the clinical and laboratory findings in Crimean Congo Haemorrhagic Fever, a viral infection characterized by activation of inflammation. PMID: 26877157
  22. The findings suggest that there are no differences in soluble TREM-1 levels in gingival crevicular fluid between healthy and periodontally diseased elderly adults PMID: 27542376
  23. TREM-1 was found to be significantly higher in tissues biopsies and blood of subjects with obesity. Greater expression and activity of TREM-1 suggest a possible role in the underlying pathophysiology of obesity and associated comorbidities. PMID: 28111922
  24. This study indicates that in children with community-acquired pneumonia, sTREM-1, Midregional-proANP, and Midregional-proADM blood levels have poor abilities to differentiate bacterial from viral diseases or to identify severe cases. PMID: 27846213
  25. Carriage of the TREM1 rs2234237T allele appears to be a risk factor for the development of severe malaria. PMID: 27671831
  26. TREM-1, HMGB1 and RAGE in the Shoulder Tendon: Dual Mechanisms for Inflammation Based on the Coincidence of Glenohumeral Arthritis PMID: 27792788
  27. is concluded that both HMGB1 and sTREM-1 are released during the inflammatory response of periodontal tissues and they can promote inflammatory process, which leads to tissue destruction. PMID: 27917624
  28. The soluble TREM-1 level, core temperature and SAPS II value were the only independent predictors of death after adjustment for potential confounders in sepsis patients admitted to intensive care. PMID: 27729010
  29. TREM-1 plays a critical role in osteoarthritis development and IL1B induced chondrocyte injury through regulation of NF-kappaB signaling PMID: 27932245
  30. sTREM1 was increased in septic patients with myocardial depression compared to those without myocardial depression. PMID: 27238916
  31. this study shows higher levels of TREM-1 in plasma of systemic lupus erythematosus in Egyptian patients PMID: 27648678
  32. Increased expression of TREM-1 in S compared to AS patients involving TNFa, MMP-1 and MMP-9 suggest a potential role of TREM-1 in plaque destabilization PMID: 27017522
  33. decreased serum level significantly associated with the presence and severity of coronary artery disease; restrains inflammatory reaction in endothelial cells PMID: 27631216
  34. Plasma levels of sTREM-1 were found to identify patients with septic shock more effectively than procalcitonin and C-reactive protein. Moreover, sTREM-1 was identified to be an early predictor for survival in patients with septic shock. PMID: 27319606
  35. This study provides new insights into the possible mechanism of TREM-1 and HIF-1alpha in psoriasis. PMID: 27743451
  36. TREM-1 may play an important role in the inflammatory cascade after subarachnoid hemorrhage and act as a monitoring biomarker PMID: 27697433
  37. This study shows increased expression of TREM-1 in monocytes from patients with advanced cervical cancer. PMID: 26506490
  38. TREM-1 expression is decreased during the generation of human osteoclast precursors PMID: 26852644
  39. TREM-1 activation may be involved in the development of Kawasaki disease. PMID: 27324541
  40. LPS-induced TREM-1 transcription. PMID: 26397033
  41. The study simultaneously evaluated both PCT and soluble TREM-1 along with CRP in febrile patients with autoimmune diseases. PMID: 27096761
  42. TREM-1 and Dectin-1 function concurrently in the corneal innate immune response by regulating inflammatory cytokine expression in fungal keratitis. PMID: 26963514
  43. High TREM-1 expression correlated significantly with increased recurrence in hepatocellular carcinoma. PMID: 25465376
  44. Soluble TREM-1 level is significantly increased in hemodialysis patients as are other pro-inflammatory markers. PMID: 26495896
  45. TREM-1 is induced by MSU and is associated with the inflammation of human acute gouty arthritis. PMID: 26320130
  46. Plasma and urine TREM-1 levels can be used as diagnostic biomarkers for acute kidney injury in critically ill patients with sepsis. PMID: 25944130
  47. study found no association between the other investigated SNPs within TREM-1 gene and infective endocarditis risk PMID: 25213166
  48. In neonates with late-onset neonatal sepsis, soluble TREM-1 has the potential to provide an excellent predictive value for septic shock/death. PMID: 25461656
  49. Soluble TREM-1, a member of the immunoglobulin super-family, is upregulated when neutrophils are exposed to bacteria. PMID: 25294884
  50. isolated PMNs have an increased proportion of both TREM1 and DAP12 compared to normal healthy control PMID: 25642940
  51. A reciprocal decrease of the pro-inflammatory surface receptor TREM-1 linked with sepsis-induced immunosuppression may be part of the explanation PMID: 25532536
  52. 25(OH)2D3-induced expression of TREM-1 was inhibited by rapamycin, a specific inhibitor of the mammalian target of rapamycin (mTOR) signaling pathway, indicating the involvement of mTOR. PMID: 25448705
  53. TREM-1 is a member of the immunoglobulin superfamily released from phagocytic cells in the presence of bacterial and fungal infections. PMID: 26281323
  54. plays critical roles in fungal infection of corneal epithelial cells PMID: 25242387
  55. Data indicate that plasma concentration of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) predicts death at 2 years after myocardial infarction. PMID: 25840803
  56. Soluble TREM-1 can serve as a biomarker for diagnosing sepsis and assessing disease severity. PMID: 25319790
  57. signaling contributes to the regulation of innate inflammatory responses PMID: 24281714
  58. Variants within the TREM locus are associated with pathological features of AD and aging-related cognitive decline. PMID: 25545807
  59. The role for PGLYRP1 as a TREM-1 activator provides a new mechanism by which bacteria can trigger myeloid cells, linking two known, but previously unrelated, pathways in innate immunity. PMID: 25595774
  60. Knockdown of TREM-1 by RNA interference inhibits cell differention but has no effect on cell-cell fusion. PMID: 24026310
  61. Soluble TREM-1 is an accurate marker of Lower respiratory tract infections. The overall diagnostic value of sTREM-1 for community acquired and hospital-acquired Lower respiratory tract infections is similar. PMID: 24661408
  62. Unlike CRP levels and ESR, serum sTREM-1 levels were not correlated with endoscopic activity in patients with intestinal Behcet's disease. PMID: 24954324
  63. TREM-1 is induced in tumor associated macrophages by cyclo-oxygenase pathway in human non-small cell lung cancer PMID: 24842612
  64. elevated in cholesteatoma PMID: 24702227
  65. sTREM-1 and procalcitonin have roles in sepsis and may predict sepsis mortality PMID: 24672147
  66. Biofilm-challenged MM6 cells exhibited higher TREM-1 expression. PMID: 24924298
  67. gene expression is modulated in the process of dengue infection PMID: 24447863
  68. Data indicate that single nucleotide polymorphisms of Triggering Receptor Expressed on Myeloid Cells-1 (TREM-1) were significantly associated with elevated risk of coronary artery disease (CAD). PMID: 25128583
  69. The gene expression levels of TREM1 in PMNs isolated from patients with bacterial infections may be used as a surrogate biomarker for determining the severity. PMID: 24465168
  70. Elevated sTREM-1 could be considered an early marker for neonatal sepsis that reflects sepsis severity and poor prognosis. PMID: 24290866
  71. The serum level of sTREM-1 may be a useful marker of disease activity in relapsing polychondritis. PMID: 24261769
  72. Studied sTREM-1, procalcitonin and C-reactive protein in postmortem serum in a series of sepsis-related fatalities and control individuals who underwent medico-legal investigations. PMID: 24012914
  73. upregulated by 1,25-dihydroxyvitamin D3 in human monocytes/macrophages PMID: 24012964
  74. Data indicate a positive correlation between serum levels of soluble TREM-1 (sTREM-1) and disease severity in infected patients as well as in experimentally infected mice. PMID: 23571837
  75. Soluble TREM-1 levels in serum were useful for the diagnosis of ventilator-associated pneumonia after cardiac surgery. PMID: 24289157
  76. Level of sTREM-1 in blood sera of patients with severe thermic injury could be proposed as an additional laboratory marker of burn wound mixed microbial infection. PMID: 24605677
  77. Serum sTREM-1 may have a role in myocardial dysfunction in patients with severe sepsis PMID: 23861562
  78. Studies indicate that bronchoalveolar lavage fluid (BALF) sTREM-1 is a useful biomarker of bacterial lung infections in in intensive care unit (ICU) patients. PMID: 23734253
  79. We found an increased oxidative stress as well as an increased expression of TREM-1 and serum levels of sTREM-1 in patients with critical limb ischemia. PMID: 23880194
  80. sTREM-1 concentrations in maternal serum were elevated during spontaneous term and preterm labor and sTREM-1 levels were significantly higher in preterm labor. PMID: 23468854
  81. dynamic change in serum sTREM- 1 may be a more accurate parameter for the assessment of sepsis prognosis; there is no association between variations rs2234246 and rs2234237 in the TREM-1 gene and susceptibility to sepsis; TREM-1 rs2234237 polymorphism is associated with increased 28-day mortality in sepsis PMID: 22798017
  82. Patients with E. coli sepsis are characterized by an association of Trem-1 expression on blood neutrophils with cytokine inducibility. PMID: 23414215
  83. Patients with sepsis had increased soluble TREM-1 and decreased TREM-1 expression on neutrophils compared to systemic inflammatory response syndrome patients. PMID: 23046618
  84. Activation of TREM-1 protected monocytic cells from apoptosis through activation of extracellular signal-regulated kinase and v-akt murine thymoma viral oncogene homologue pathways and MCL1 PMID: 23364598
  85. TREM-1 induction by the hypoxic microenvironment represents a mechanism of regulation of Th1-cell trafficking and activation by iDCs differentiated at pathologic sites. PMID: 23436478
  86. Gene expression in 23 patients (12 with pneumonia and 11 w/sepsis) were analyzed using quantitative real time pcr. The mRNA levels of TLR2 (20/23 cases) and CD14 (18/23 cases) were upregulated in the PMNs of patients when compared with healthy subjects. PMID: 22996209
  87. In ulcerative colitis, sTREM-1 level was correlated most closely with the endoscopic disease activity among serum biomarkers, but was not superior to the clinical activity index. PMID: 22721842
  88. Patients with rheumatoid arthritis had higher plasma sTREM-1 levels than controls, and plasma sTREM-1 levels were correlated with disease activity, suggesting that plasma sTREM-1 could play a role in the inflammatory process associated with TNF-alpha. PMID: 22422501
  89. Elevated plasma levels of sTREM-1 reflect the severity, state of exacerbation, presence of respiratory tract obstruction in allergic asthma bronchiale patients. PMID: 22829716
  90. levels of sTREM-1 were significantly higher in patients with epatocellular carcinoma (HCC) than those with benign liver tumors; peritumoral density of TREM-1 was shown to be an independent prognosis predictor; observations suggest that TREM-1 is related to the aggressive tumor behavior and has potential value as a prognostic factor for HCC PMID: 22417086
  91. Although serum levels of sTREM-1 are increased early upon advent of severe sepsis/shock, gene expression of TREM-1 on monocytes in severe sepsis/shock is not increased. PMID: 21855288
  92. Our finding that TREM-1 controls the production of interleukin-8 and TNF-alpha in U937 foam cells defines a potentially critical role of TREM-1 in the pathogenesis of atherosclerosis PMID: 22642593
  93. the sustained TREM-1 expression at the cell surface suffices to block the progress of a refractory state in human monocytes PMID: 22459945
  94. study shows an increase in sTREM-1 in patients with COPD compared to smokers but not in acute exacerbation-COPD compared to stable COPD. Viral exacerbations showed significantly lower sTREM-1 levels than non-viral exacerbations. PMID: 21701149
  95. Data demonstrate that sTREM-1 is elevated in high-risk patients with febrile neutropenia and is potentially useful to predict their clinical course, either together with, or as an alternative to procalcitonin. PMID: 21976106
  96. Serum and amniotic fluid sTREM-1 levels may emerge as early biological indicators for predicting PROM complicated with subclinical chorioamnionitis. PMID: 22179409
  97. Data show that kinetics of sTREM-1 differs among patients undergoing hemiarthroplasty of the hip and those undergoing total hip arthroplasty. PMID: 21942576
  98. High sTREM-1 is associated with intestinal Behcet's disease. PMID: 21910175
  99. the putative periodontal pathogen P. gingivalis can positively regulate the expression of the TREM-1/DAP12 pathway in monocytic cells. PMID: 21967868
  100. TREM-1 gene expression was increased in the monocytes of schizophrenic and bipolar patients & tended to be increased in the monocytes of major depressive disorder patients. PMID: 21421043

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Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Secreted
Tissue Specificity Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in norma
Database Links

HGNC: 17760

OMIM: 605085

KEGG: hsa:54210

STRING: 9606.ENSP00000244709

UniGene: Hs.283022

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