Code | CSB-E04836h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human TREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 97 | ||||
Range % | 92-102 | |||||
1:2 | Average % | 90 | ||||
Range % | 82-97 | |||||
1:4 | Average % | 104 | ||||
Range % | 99-110 | |||||
1:8 | Average % | 86 | ||||
Range % | 82-93 |
The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 84-96 | ||||
EDTA plasma (n=4) | 96 | 90-101 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
CUSABIO's human TREM1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for quantitatively measuring human TREM1 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of TREM1 in samples. The color intensity is measured at 450 nm via a microplate reader.
TREM1, an activating receptor expressed on myeloid cells, has the synergistic ability to amplify the signaling of the TLR4 or TLR2, which can recognize components of a variety of microorganisms including bacteria, fungi, and viruses. TREM1 mainly augments inflammation during acute inflammation and is upregulated during sepsis and pulmonary infection. It has been recognized as a critical immunomodulator in several inflammatory diseases of both infectious and non-infectious etiologies such as inflammatory bowel disease, acute pancreatitis, gouty arthritis, and atherosclerosis.
There are currently no reviews for this product.