Human Trypsin-3 (PRSS3) ELISA kit

Code CSB-EL018820HU
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Product Type ELISA kit
Size 96T,5×96T,10×96T
Uniprot No. P35030
Lead Time 3-5 working days
Abbreviation PRSS3
Target Name Trypsin-3 (PRSS3)
Alias Brain trypsinogen Mesotrypsinogen Serine protease 3 Serine protease 4 Trypsin III Trypsin IV PRSS4, TRY3, TRY4
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 92
Range % 87-96
1:200 Average % 103
Range % 98-107
1:400 Average % 105
Range % 101-109
1:800 Average % 92
Range % 86-98
Recovery
The recovery of human PRSS3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 86-98
EDTA plasma (n=4) 97 91-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.584 2.687 2.636 2.552
500 2.295 2.165 2.230 2.146
250 1.594 1.523 1.559 1.475
125 0.889 0.876 0.883 0.799
62.5 0.441 0.454 0.448 0.364
31.2 0.261 0.252 0.257 0.173
15.6 0.143 0.148 0.146 0.062
0 0.083 0.084 0.084  
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Function Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5.
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Expressed in pancreas and brain. Also expressed in Paneth cells, at the base of small intestinal crypts.
Database Links

HGNC: 9486

OMIM: 613578

KEGG: hsa:5646

STRING: 9606.ENSP00000354280

UniGene: Hs.654513

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