Human Trypsin-3 (PRSS3) ELISA kit

Code CSB-EL018820HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Human PRSS3 ELISA Kit was designed for the quantitative measurement of Human PRSS3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Name Trypsin-3 (PRSS3)
Alternative Names
Brain trypsinogen ELISA Kit; Mesotrypsin ELISA Kit; Mesotrypsinogen ELISA Kit; MTG ELISA Kit; Pancreatic trypsinogen III ELISA Kit; Protease, serine, 3 ELISA Kit; Protease, serine, 4 (trypsin 4, brain) ELISA Kit; PRSS3 ELISA Kit; PRSS4 ELISA Kit; Serine protease 3 ELISA Kit; Serine protease 4 ELISA Kit; T9 ELISA Kit; TRY3 ELISA Kit; TRY3_HUMAN ELISA Kit; TRY4 ELISA Kit; Trypsin 3 ELISA Kit; Trypsin III ELISA Kit; Trypsin IV ELISA Kit; Trypsin-3 ELISA Kit; Trypsinogen 4 ELISA Kit; Trypsinogen 5 ELISA Kit; Trypsinogen IV ELISA Kit
Abbreviation PRSS3
Uniprot No. P35030
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 92
Range % 87-96
1:200 Average % 103
Range % 98-107
1:400 Average % 105
Range % 101-109
1:800 Average % 92
Range % 86-98
The recovery of human PRSS3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 86-98
EDTA plasma (n=4) 97 91-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.584 2.687 2.636 2.552
500 2.295 2.165 2.230 2.146
250 1.594 1.523 1.559 1.475
125 0.889 0.876 0.883 0.799
62.5 0.441 0.454 0.448 0.364
31.2 0.261 0.252 0.257 0.173
15.6 0.143 0.148 0.146 0.062
0 0.083 0.084 0.084  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Digestive protease that cleaves proteins preferentially after an Arg residue and has proteolytic activity toward Kunitz-type trypsin inhibitors.
Gene References into Functions
  1. PRSS3 is downregulated by intragenic hypermethylation in HCC. * Epigenetic silencing of PRSS3 facilitates growth, migration, and invasion of HCC. * PRSS3 intragenic methylation has implication in diagnosis of HCC. PMID: 28844099
  2. PRSS3 acts as an oncogene in invasive ductal carcinoma of the breast development and progression PMID: 28423522
  3. Study developed a new high resolution crystal structure of mesotrypsin complexed with diminazene through a structure-based molecular docking screen that could help facilitate derivatization efforts, addressing current pan-inhibition of human trypsins through rigidification of diminazene to select for a conformation that maximizes interactions with the non-conserved Arg-193 residue. PMID: 28463992
  4. Data show that silencing tumor-endothelial cells (EC) for trypsinogen 4 accumulated tissue factor pathway inhibitor-2 (TFPI-2) in the matrix. PMID: 26318044
  5. These findings suggest that inhibitor cleavage represents a functional adaptation of mesotrypsin that may have evolved in response to positive selection pressure. PMID: 26175157
  6. High PRSS3 expression in EOC tissues was significantly associated with advanced FIGO stage and lymph node metastasis. PMID: 25735255
  7. Data suggest that mesotrypsin cleavage of Kunitz domains may contribute to cancer progression. PMID: 25301953
  8. Mesotrypsin generated saposins A-D from prosaposin, and mature caspase-14 contributed to this process by activating mesotrypsinogen to mesotrypsin. Knockdown of these proteases markedly down-regulated saposin A synthesis in skin equivalent models. PMID: 24872419
  9. extra-pancreatic trypsinogen 3 is produced by esophageal adenocarcinoma cells and activates PAR-2 in an autocrine manner. PMID: 24146905
  10. IFN regulatory factor 2 (Irf2) has a regulatory role in trypsinogen5 gene transcription, which is resistant to a major endogenous trypsin inhibitor, Spink3 PMID: 22042864
  11. Report PRSS3/mesotrypsin upregulation in breast cancer cells and identify CD109 as the functional proteolytic target of mesotrypsin. PMID: 20035377
  12. PRSS3 plays an important role in the progression, metastasis and prognosis of human pancreatic cancer. PMID: 20947888
  13. Investigation did not reveal an association between PRSS3 variants and chronic pancreatitis. PMID: 20484962
  14. Because mesotrypsin is resistant to naturally occurring trypsin inhibitors, confined expression of the isoforms of mesotrypsinogens and enteropeptidase may indicate that mesotrypsin is involved in keratinocyte terminal differentiation PMID: 19924134
  15. Processing by mesotrypsin may ablate the protease inhibitory function of APP/protease nexin 2 in vivo and may also modulate other activities of APP/protease nexin 2 that involve the Kunitz domain. PMID: 19920152
  16. X-ray structure in complex with the inhibitor benzamidine at 1.7 A resolution; crystal structure reveals basis for inhibitor resistance PMID: 11827488
  17. biological function of human mesotrypsin is digestive degradation of trypsin inhibitors PMID: 14507909
  18. The results classify E32del mesotrypsinogen as a frequent polymorphic variant, which is not associated with chronic alcoholic pancreatitis PMID: 15855826
  19. PRSS3 promoter methylation is associated with advanced bladder cancer PMID: 15987713
  20. we determined the promoter hypermethylation status of PRSS3 in a case series study of primary NSCLC, and found methylation of this gene to be common, occurring in 53% (86 of 166) of tumors examined. PMID: 16013053
  21. Results suggest that human trypsin 4 may be one of the candidate proteases involved in the pathomechanism of multiple sclerosis via cleavage of myelin basic protein. PMID: 16412431
  22. analysis of structural rearrangement during the acylation step in human trypsin 4 on 4-methylumbelliferyl 4-guanidinobenzoate substrate analogue PMID: 16492676
  23. mesotrypsin cannot activate pancreatic zymogens, but might activate certain proteinase-activated receptors because of its thrombin-like subsite specificity; alpha1AT Pittsburgh is an effective mesotrypsin inhibitor PMID: 16759229
  24. human trypsinogen 4 is widely but unevenly distributed in the human brain. It is localized in neurons and glial cells, predominantly in astrocytes & the extracellular matrix. PMID: 17406981
  25. trypsin IV and p23 are inhibitor-resistant trypsins that can cleave and activate PARs, causing PAR(1)- and PAR(2)-dependent inflammation and PAR(2)-dependent hyperalgesia. PMID: 17623652
  26. This study reveals enhanced mRNA expression of trypsinogen IV and SERT and a higher 5-HT content in the small intestine of IBS patients compared to healthy subjects. PMID: 18363639
  27. Absence of mesotrypsinogen gene (PRSS3) copy number variations in patients with chronic pancreatitis. PMID: 18665091
  28. Here, we report that nexin-1 inhibits trypsin-4, and forms stable complexes only with this trypsin-isoenzyme. This result suggests that nexin-1 could modulate trypsin activity in brain where both nexin-1 and trypsin-4 are expressed. PMID: 19249338

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Subcellular Location Secreted.
Protein Families Peptidase S1 family
Tissue Specificity Detected in pancreas and pancreatic fluid (at protein level). Expressed in pancreas and brain. Detected in ileum.
Database Links

HGNC: 9486

OMIM: 613578

KEGG: hsa:5646

STRING: 9606.ENSP00000354280

UniGene: Hs.654513

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