Human apolipoprotein A1 (Apo-A1) ELISA Kit

Code CSB-E08103h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P02647
Lead Time 3-5 working days
Protein Biological Process 2 Lipogenesis and lipometabolism
Abbreviation APOA1
Protein Biological Process 1 Apolipoprotein
Target Name apolipoprotein A-I
Alias MGC117399, OTTHUMP00000069346|OTTHUMP00000069347|OTTHUMP00000069348
Species Homo sapiens (Human)
Protein Biological Process 3 Cholesterol metabolism
Sample Types serum, plasma, cell culture supernates, saliva, urine
Detection Range 1.4 ng/mL-1000 ng/mL
Sensitivity 0.7 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.
HGNC 600
RGD 2130
MGI 88049
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:2000 Average % 88  
Range % 84-92  
1:4000 Average % 96  
Range % 92-102  
1:8000 Average % 95  
Range % 89-100  
1:16000 Average % 92  
Range % 89-96  
The recovery of human Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 96 91-101  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
1000 1.879 1.903 1.891 1.728  
500 1.592 1.612 1.602 1.439  
250 1.304 1.311 1.308 1.145  
125 0.955 0.968 0.962 0.799  
62.5 0.743 0.757 0.750 0.587  
31.2 0.562 0.572 0.567 0.404  
15.6 0.374 0.376 0.375 0.212  
0 0.161 0.165 0.163    
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Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Involvement in disease High density lipoprotein deficiency 2 (HDLD2); High density lipoprotein deficiency 1 (HDLD1); Amyloidosis 8 (AMYL8)
Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b
Database Links

HGNC: 600

OMIM: 105200

KEGG: hsa:335

STRING: 9606.ENSP00000236850

UniGene: Hs.93194

Pathway PPAR signaling pathway
Cholesterol metabolism

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