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| Code | CSB-E08103h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:2000 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:4000 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:8000 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:16000 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| The recovery of human Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 91-101 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human APOA1 ELISA Kit is suitable for qualitatively determining human concentrations in serum, plasma, cell culture supernates, saliva, or urine in vitro. APOA1 is the main structural protein in high-density lipoprotein (HDL). It is synthesized by the liver (70%) and small intestine (30%) in a lipid-poor state and then packed into HDL by adding phospholipids and unesterified cholesterol aided by the ABCA1 at the cell surface, forming nascent HDL. It has over 90% amphipathic α-helical content, allowing for the formation and stabilization of the HDL. HDL quality is highly dependent on the abundance and function of ApoA1. APOA1 is primarily involved in reverse cholesterol transport. APOA1 also plays important role in tumor growth, angiogenesis, and invasion, and metastasis.
This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human APOA1 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human APOA1 antibody. Biotin-labeled APOA1 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the APOA1 in the rat samples. APOA1 binds to the biotinylated anti-APOA1 human monoclonal antibody. And the biotin on the biotinylated anti-APOA1 human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of APOA1 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.
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