Human cytokeratin 18,CK-18 ELISA Kit

Code CSB-E09172h
Size 96T,5×96T,10×96T
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Product Details

Target Name
keratin 18
Alternative Names
Cell proliferation inducing gene 46 protein ELISA Kit; Cell proliferation inducing protein 46 ELISA Kit; Cell proliferation-inducing gene 46 protein ELISA Kit; CK 18 ELISA Kit; CK-18 ELISA Kit; CK18 ELISA Kit; CYK 18 ELISA Kit; CYK18 ELISA Kit; Cytokeratin 18 ELISA Kit; Cytokeratin endo B ELISA Kit; Cytokeratin-18 ELISA Kit; K 18 ELISA Kit; K18 ELISA Kit; K1C18_HUMAN ELISA Kit; KA18 ELISA Kit; Keratin 18 ELISA Kit; Keratin 18, type I ELISA Kit; Keratin D ELISA Kit; keratin, type I cytoskeletal 18 ELISA Kit; Keratin-18 ELISA Kit; Krt18 ELISA Kit
Abbreviation
KRT18
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates, cell culture supernates
Detection Range
0.625 ng/mL-15 ng/mL
Sensitivity
0.25 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<10%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CK-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-100  
1:2 Average % 92  
Range % 88-99  
1:4 Average % 97  
Range % 92-106  
1:8 Average % 94  
Range % 86-103  
Recovery
The recovery of human CK-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 89-102  
EDTA plasma (n=4) 97 92-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
15 1.932 1.958 1.945 1.898  
7.5 1.318 1.357 1.338 1.291  
3.75 0.786 0.798 0.792 0.745  
1.25 0.375 0.362 0.369 0.322  
0.625 0.216 0.223 0.220 0.173  
0 0.046 0.047 0.047    
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-glucagon antibody.
  • Six vials standard (5ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial HRP-conjugated glucagon antibody (6 ml/bottle) --- Bind to the glucagon in the samples or standards and react with the substrate to make the solution chromogenic.
  • One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.
  • One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.
  • One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.
  • One vial Stop Solution (7ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human KRT18 ELISA Kit was designed for the quantitative measurement of Human KRT18 protein in serum, plasma, tissue homogenates, cell lysates, cell_x005f_x000D_ culture supernates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-15 ng/mL and the sensitivity is 0.25 ng/mL.

Customer Reviews and Q&A

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 Q&A
Q:

Could you please advice if you supply the quality control for this kit?Hope to hear from you soon.I am referring to the high and low control. Is it included in the kit?

A:
Thanks for your inquiry. Sorry the kit does not include the mentioned control.But it contains the standard in it which is used to validate and analyze the whole reagent system and can work as controls.

Target Background

Function
(From Uniprot)
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Gene References into Functions
  1. the present study demonstrated that CK18 might be served as a novel biomarker to predict clinicopathological features and the outcome of breast cancer. PMID: 29437899
  2. This study analyzed phase III data comparing basal insulin peglispro (BIL) and insulin glargine in type 1 (T1D), and type 2 diabetes (T2D) (insulin-naive and insulin-treated). Alanine aminotransferase (ALT), K-18, enhanced liver fibrosis scores and liver fat content. PMID: 29167192
  3. Results found that CK18, MMP-9, and TIMP1 averages of those with positive clinical lymph nodes and those in clinical stage 3 were higher than the averages of those with negative clinical lymph nodes and those in clinical stage 2. PMID: 29651326
  4. non-surviving severe malignant middle cerebral artery infarction patients had higher serum CCCK-18 levels than surviving patients PMID: 29573748
  5. Plasma M30-M65 levels are higher in serum of patients with placental abruption. PMID: 27893299
  6. results show that the caspase digestion-resistant K18 helps to maintain keratin filament organization and delays apoptosis, thereby resulting in protection from liver injury. PMID: 28606991
  7. The plasma keratin-18 (K18) values of nonsurviving alcoholic hepatitis (AH) patients were significantly elevated above their surviving counterparts and healthy controls PMID: 28770701
  8. Evidence suggested that the modification of histone H3 was highly correlated with the modulation of cytokeratin 18 and probably plays an important role in tumorigenesis of hepatocytes PMID: 28647696
  9. K8/K18 interact with Notch1 and regulate Notch1 signalling activity during differentiation of the colonic epithelium. PMID: 28475172
  10. FIB-4 index and CK-18F have good diagnostic abilities not only for nonalcoholic steatohepatitis (NASH) overall, but also for NASH with mild fibrosis. PMID: 29040984
  11. In children with nonalcoholic fatty liver disease, CK18 levels were found to be significantly higher in subjects with any fibrosis compared with those without fibrosis (304.6 +/- 124.8 vs 210.4 +/- 70.9, P < 0.001). PMID: 26835904
  12. CK18 combined with uric acid measurement is a promising non-invasive biomarker for prediction of disease severity in NASH patients PMID: 28472039
  13. Results suggested that serum M65 is a useful indicator of liver inflammation in chronic hepatitis B patients. Serum M65, not M30, is valuable in the grading of liver fibrosis. PMID: 28514295
  14. The results suggest that increased circulating CK-18 and FGF-21 are associated with Nonalcoholic Fatty Liver Disease and may be used for initial assessment, but not enough PMID: 28326329
  15. The purpose of this study is to investigate the immunohistochemical expression of cytokeratin 18 (CK18) and the reactivity to GDF5 (CDMP-1), called the morphogenetic protein-1, cartilage-derived, in lingual squamous cell carcinoma. PMID: 27151703
  16. In patients with anterior STEMI treated with primary PCI, the apoptosis marker M30 might be useful for predicting LV remodeling and subsequent LV systolic dysfunction. PMID: 27479531
  17. Serum concentrations of CK-18 fragments and transgelin-2 correlate with the severity of NAFLD, but not with obesity. PMID: 27740519
  18. High KRT18 serum levels are associated with non-small-cell lung cancer. PMID: 27468866
  19. Serum HGF and CK18 levels were significantly up-regulated and positively correlated with metastasis stage, tumor stage, and disease stage of esophageal squamous cell carcinoma. PMID: 27706656
  20. CK18 plays an important role in lung cancer progression and may be a therapeutic target for non small cell lung cancer. PMID: 27601168
  21. these results suggest that cytokeratin 18 critically contributes to initiating TGF-beta1-induced EMT via the smad 2/3-mediated regulation of snail and slug expression in breast epithelial cells. PMID: 27734227
  22. Study demonstrated that the downregulation of CK18 is associated with the acquisition of paclitaxel-resistance and tumor aggressiveness in prostate cancer. PMID: 26892177
  23. Data indicate that O-GlcNAcylation at serine Ser30 determines the occurrence of Pposphorylation at Ser33 on Keratin 18 (K18). PMID: 27059955
  24. Data show that adipose-derived stem cells (ADSC) differentiate into epithelial-like cells with the expression of epithelial surface marks cytokeratin 18 and 19 and form tube-like structures. PMID: 26416346
  25. High circulating CCCK-18 levels were associated with injury severity and a poor clinical outcome after aSAH and CCCK-18 had the potential to be a good prognostic biomarker for aSAH. PMID: 26671131
  26. NAFLD patients, serum CK-18 levels reflect disease activity scores and correlate with histological changes. PMID: 26436355
  27. Data show that the decrease of keratin 18 (K18) ser33 and ser52 phosphorylation inhibited autophagy and decreased apoptosis of HCT116 cells. PMID: 26728375
  28. As an apoptosis-related biomarker, serum M30 [M30 cytokeratin-18 peptide, human ] level has a merit to be the most promising test for prediction or differential diagnosis of HELLP syndrome in PE patients. PMID: 26138305
  29. Serum caspase-cleaved cytokeratin-18 levels are associated with 30-day mortality and could be used as a prognostic biomarker in patients with severe traumatic brain injury. PMID: 25822281
  30. The results suggest that oblongifolin C inhibits metastasis through the induction of the expression of keratin 18 and may be useful in cancer therapy. PMID: 25973684
  31. Our results suggest that reliable detection of apoptosis via the M30 assay only works when sufficient levels of cytokeratin 18 are present in the cells. This means that the M30 assay may result in false negative results for apoptosis PMID: 25846732
  32. No evidence of association between polymorphisms in KRT18 and sporadic amyotrophic lateral sclerosis in Han Chinese PMID: 25677198
  33. Variants in highly conserved residues of K18 Asp89His (isoniazid-related) were found in patients with fatal Drug-induced liver injury. PMID: 26286715
  34. Serum caspase-cleaved cytokeratin-18 levels were associated with mortality in severe septic patients. PMID: 25290885
  35. Although only serum M65 levels were found to be of diagnostic value, neither M30 nor M65 serum levels played a prognostic role in the outcome in melanoma patients. PMID: 23812330
  36. Under the present study design propofol or sevoflurane anaesthesia did not induce apoptosis or affected the liver function as assessed by the M30, M65 markers and liver enzymes in patients undergoing mastectomy or thyroidectomy under general anaesthesia. PMID: 25579144
  37. There were no statistically significant correlations between serum M30 and M65 concentrations and clinicodemographical variables. Serum M30 and M65 concentrations were found to have a diagnostic value in nasopharyngeal cancer PMID: 25326440
  38. The broken dialog between ANXA1 and CK18 in normal breast tissues may play a critical role in breast cancer development. PMID: 25028131
  39. The distribution of CK18 in a large cohort of older people with type 2 diabetes is associated with an adverse metabolic risk factor profile. PMID: 24237940
  40. placental expression significantly increased in preeclampsia compared to normotensive pregnancies PMID: 24857367
  41. Full length and caspase cleaved cytokeratin 18 (detected as M65 and M30 antigens) are associated with prognosis in hepatitis B virus-related acute-on-chronic liver failure. PMID: 24605039
  42. KRT18 expression is directly regulated by EGR1, and contributes to decrease malignancy of non-small cell lung carcinoma. PMID: 24990820
  43. we identified four proteins with different expression in paclitaxel resistant cells, i.e., serpin B3, serpin B4, heat shock protein 27 (all three upregulated) and cytokeratin 18 (downregulated). PMID: 24898082
  44. effect between the CK-18 concentration and the severity of NAFLD was found in the T2DM patients PMID: 24667697
  45. Due to promising results of various studies, CK-18 cell death markers could be applied in clinical routine soon. PMID: 24622872
  46. Median serum concentration of M30-CK18 was higher in chronic hepatitis C patients and was associated with inflammatory activity and liver fibrosis. PMID: 23981197
  47. A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients. PMID: 24324749
  48. Serum M65 has the potential as a new diagnostic parameter for hepatocellular carcinoma (HCC) and serum CD163 is a new prognostic parameter in HCC patients. PMID: 23792028
  49. M 30 serum levels increase in patients with active ulcerative colitis. PMID: 23945185
  50. These results provide an explanation for the elevated serum levels of HSP-27, HSP-70, and ccCK-18 found in COPD patients, indicating that hypoxic conditions can trigger the release of the aforementioned factors. PMID: 24660549

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Involvement in disease
Cirrhosis (CIRRH)
Subcellular Location
Cytoplasm, perinuclear region. Nucleus, nucleolus.
Protein Families
Intermediate filament family
Tissue Specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Database Links

HGNC: 6430

OMIM: 148070

KEGG: hsa:3875

STRING: 9606.ENSP00000373487

UniGene: Hs.406013

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