Recombinant Human KeRatin, type I cytoskeletal 18(KRT18),partial

Code CSB-EP012519HU2
Size US$1726
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names KRT18
Uniprot No. P05783
Research Area Cell Cycle
Alternative Names Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46 ; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; K 18; K18; K1C18_HUMAN; KA18; Keratin 18; Keratin 18, type I; Keratin D ; keratin, type I cytoskeletal 18; Keratin-18; Krt18
Species Homo sapiens (Human)
Source E.coli
Expression Region 102-396aa
Target Protein Sequence ETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDAL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 38.0kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Gene References into Functions
  1. the present study demonstrated that CK18 might be served as a novel biomarker to predict clinicopathological features and the outcome of breast cancer. PMID: 29437899
  2. This study analyzed phase III data comparing basal insulin peglispro (BIL) and insulin glargine in type 1 (T1D), and type 2 diabetes (T2D) (insulin-naive and insulin-treated). Alanine aminotransferase (ALT), K-18, enhanced liver fibrosis scores and liver fat content. PMID: 29167192
  3. Results found that CK18, MMP-9, and TIMP1 averages of those with positive clinical lymph nodes and those in clinical stage 3 were higher than the averages of those with negative clinical lymph nodes and those in clinical stage 2. PMID: 29651326
  4. non-surviving severe malignant middle cerebral artery infarction patients had higher serum CCCK-18 levels than surviving patients PMID: 29573748
  5. Plasma M30-M65 levels are higher in serum of patients with placental abruption. PMID: 27893299
  6. results show that the caspase digestion-resistant K18 helps to maintain keratin filament organization and delays apoptosis, thereby resulting in protection from liver injury. PMID: 28606991
  7. The plasma keratin-18 (K18) values of nonsurviving alcoholic hepatitis (AH) patients were significantly elevated above their surviving counterparts and healthy controls PMID: 28770701
  8. Evidence suggested that the modification of histone H3 was highly correlated with the modulation of cytokeratin 18 and probably plays an important role in tumorigenesis of hepatocytes PMID: 28647696
  9. K8/K18 interact with Notch1 and regulate Notch1 signalling activity during differentiation of the colonic epithelium. PMID: 28475172
  10. FIB-4 index and CK-18F have good diagnostic abilities not only for nonalcoholic steatohepatitis (NASH) overall, but also for NASH with mild fibrosis. PMID: 29040984
  11. In children with nonalcoholic fatty liver disease, CK18 levels were found to be significantly higher in subjects with any fibrosis compared with those without fibrosis (304.6 +/- 124.8 vs 210.4 +/- 70.9, P < 0.001). PMID: 26835904
  12. CK18 combined with uric acid measurement is a promising non-invasive biomarker for prediction of disease severity in NASH patients PMID: 28472039
  13. Results suggested that serum M65 is a useful indicator of liver inflammation in chronic hepatitis B patients. Serum M65, not M30, is valuable in the grading of liver fibrosis. PMID: 28514295
  14. The results suggest that increased circulating CK-18 and FGF-21 are associated with Nonalcoholic Fatty Liver Disease and may be used for initial assessment, but not enough PMID: 28326329
  15. The purpose of this study is to investigate the immunohistochemical expression of cytokeratin 18 (CK18) and the reactivity to GDF5 (CDMP-1), called the morphogenetic protein-1, cartilage-derived, in lingual squamous cell carcinoma. PMID: 27151703
  16. In patients with anterior STEMI treated with primary PCI, the apoptosis marker M30 might be useful for predicting LV remodeling and subsequent LV systolic dysfunction. PMID: 27479531
  17. Serum concentrations of CK-18 fragments and transgelin-2 correlate with the severity of NAFLD, but not with obesity. PMID: 27740519
  18. High KRT18 serum levels are associated with non-small-cell lung cancer. PMID: 27468866
  19. Serum HGF and CK18 levels were significantly up-regulated and positively correlated with metastasis stage, tumor stage, and disease stage of esophageal squamous cell carcinoma. PMID: 27706656
  20. CK18 plays an important role in lung cancer progression and may be a therapeutic target for non small cell lung cancer. PMID: 27601168
  21. these results suggest that cytokeratin 18 critically contributes to initiating TGF-beta1-induced EMT via the smad 2/3-mediated regulation of snail and slug expression in breast epithelial cells. PMID: 27734227
  22. Study demonstrated that the downregulation of CK18 is associated with the acquisition of paclitaxel-resistance and tumor aggressiveness in prostate cancer. PMID: 26892177
  23. Data indicate that O-GlcNAcylation at serine Ser30 determines the occurrence of Pposphorylation at Ser33 on Keratin 18 (K18). PMID: 27059955
  24. Data show that adipose-derived stem cells (ADSC) differentiate into epithelial-like cells with the expression of epithelial surface marks cytokeratin 18 and 19 and form tube-like structures. PMID: 26416346
  25. High circulating CCCK-18 levels were associated with injury severity and a poor clinical outcome after aSAH and CCCK-18 had the potential to be a good prognostic biomarker for aSAH. PMID: 26671131
  26. NAFLD patients, serum CK-18 levels reflect disease activity scores and correlate with histological changes. PMID: 26436355
  27. Data show that the decrease of keratin 18 (K18) ser33 and ser52 phosphorylation inhibited autophagy and decreased apoptosis of HCT116 cells. PMID: 26728375
  28. As an apoptosis-related biomarker, serum M30 [M30 cytokeratin-18 peptide, human ] level has a merit to be the most promising test for prediction or differential diagnosis of HELLP syndrome in PE patients. PMID: 26138305
  29. Serum caspase-cleaved cytokeratin-18 levels are associated with 30-day mortality and could be used as a prognostic biomarker in patients with severe traumatic brain injury. PMID: 25822281
  30. The results suggest that oblongifolin C inhibits metastasis through the induction of the expression of keratin 18 and may be useful in cancer therapy. PMID: 25973684
  31. Our results suggest that reliable detection of apoptosis via the M30 assay only works when sufficient levels of cytokeratin 18 are present in the cells. This means that the M30 assay may result in false negative results for apoptosis PMID: 25846732
  32. No evidence of association between polymorphisms in KRT18 and sporadic amyotrophic lateral sclerosis in Han Chinese PMID: 25677198
  33. Variants in highly conserved residues of K18 Asp89His (isoniazid-related) were found in patients with fatal Drug-induced liver injury. PMID: 26286715
  34. Serum caspase-cleaved cytokeratin-18 levels were associated with mortality in severe septic patients. PMID: 25290885
  35. Although only serum M65 levels were found to be of diagnostic value, neither M30 nor M65 serum levels played a prognostic role in the outcome in melanoma patients. PMID: 23812330
  36. Under the present study design propofol or sevoflurane anaesthesia did not induce apoptosis or affected the liver function as assessed by the M30, M65 markers and liver enzymes in patients undergoing mastectomy or thyroidectomy under general anaesthesia. PMID: 25579144
  37. There were no statistically significant correlations between serum M30 and M65 concentrations and clinicodemographical variables. Serum M30 and M65 concentrations were found to have a diagnostic value in nasopharyngeal cancer PMID: 25326440
  38. The broken dialog between ANXA1 and CK18 in normal breast tissues may play a critical role in breast cancer development. PMID: 25028131
  39. The distribution of CK18 in a large cohort of older people with type 2 diabetes is associated with an adverse metabolic risk factor profile. PMID: 24237940
  40. placental expression significantly increased in preeclampsia compared to normotensive pregnancies PMID: 24857367
  41. Full length and caspase cleaved cytokeratin 18 (detected as M65 and M30 antigens) are associated with prognosis in hepatitis B virus-related acute-on-chronic liver failure. PMID: 24605039
  42. KRT18 expression is directly regulated by EGR1, and contributes to decrease malignancy of non-small cell lung carcinoma. PMID: 24990820
  43. we identified four proteins with different expression in paclitaxel resistant cells, i.e., serpin B3, serpin B4, heat shock protein 27 (all three upregulated) and cytokeratin 18 (downregulated). PMID: 24898082
  44. effect between the CK-18 concentration and the severity of NAFLD was found in the T2DM patients PMID: 24667697
  45. Due to promising results of various studies, CK-18 cell death markers could be applied in clinical routine soon. PMID: 24622872
  46. Median serum concentration of M30-CK18 was higher in chronic hepatitis C patients and was associated with inflammatory activity and liver fibrosis. PMID: 23981197
  47. A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients. PMID: 24324749
  48. Serum M65 has the potential as a new diagnostic parameter for hepatocellular carcinoma (HCC) and serum CD163 is a new prognostic parameter in HCC patients. PMID: 23792028
  49. M 30 serum levels increase in patients with active ulcerative colitis. PMID: 23945185
  50. These results provide an explanation for the elevated serum levels of HSP-27, HSP-70, and ccCK-18 found in COPD patients, indicating that hypoxic conditions can trigger the release of the aforementioned factors. PMID: 24660549
  51. Patients with nonalcoholic fatty liver disease had higher blood keratin-18 levels. PMID: 24067262
  52. Low expression of M30 implies the development of resistance mechanisms against apoptosis, facilitating the progression of colon cancer. PMID: 23767766
  53. Caspase-cleaved or total CK-18 are promising plasma biomarkers to identify patients with HELLP syndrome. PMID: 24157880
  54. Data indicate that although both serum levels of M30 and M65 assays were found to be of diagnostic value, no predictive and prognostic values were determined in pancreatic adenocarcinoma (MPA) patients. PMID: 23784459
  55. This first human study suggests that cytokeratin 18 (marker of apoptosis) might be a serological predictive marker for acute pancreatitis for disease activity. PMID: 23864450
  56. CK18 is a promising non-invasive biomarker for nonalcoholic steatohepatitis in children with fatty liver disease. PMID: 23752877
  57. Serum keratin-18 values increase after chemotherapy in patients with advanced non-small-cell lung cancer; elevated serum value is an independent prognostic factor for both progression-free survival and overall survival. PMID: 23536001
  58. high serum M65 levels were associated with poor prognosis and resistance to platinum-based chemotherapy PMID: 23801282
  59. Serum sFas could be considered a possible marker of advanced fibrosis both in pediatric and adult patient with chronic hepatitis C as well as M30 might be a good predictor of steatosis severity in children. PMID: 23326448
  60. Report keratin 18 expression in breast papillomas and papillary carcinoma. PMID: 23327593
  61. Low Cytokeratin 18 expression is associated with less response to therapy in non-small-cell lung cancer. PMID: 23154549
  62. There is aberrant expression of CK18 in dysplasia, oral submucous fibrosis, and oral squamous cell carcinoma, suggesting CK18 as a surrogate marker of malignant transformation. PMID: 22677743
  63. H. pylori-mediated cleavage of CK18 appeared proportional to the number of CagA EPIYA-C and CM motifs, which seemed to be downplayed in the presence of EPIYA-B motifs PMID: 22967118
  64. Keratin 18 increases CFTR expression by binding to its C-terminal hydrophobic domain. PMID: 23045527
  65. Elevated serum M30 and M65 is associated with treatment response in advanced gastric cancer. PMID: 22890829
  66. loss of cytokeratin 18 expression might be a good indicator of the poor prognosis of the breast cancer and it may play an active role in the breast tumorigenesis. PMID: 22699783
  67. There is a synergism between risky alcohol consumption and common metabolic disorders (particularly obesity) in relation to serum concentrations of cytokeratin-18. PMID: 21682752
  68. A dose-dependent increase in the activities of caspase-9 and caspase-3 coinciding with elevation of CK18 levels was found in bromelain-treated breast cancer cells. PMID: 22191568
  69. Redox regulation of cytokeratin 18 protein by NADPH oxidase 1 in preneoplastic human epithelial cells PMID: 21877197
  70. Suggest that CK18 is unsuitable for early diagnosis of tongue squamous cell carcinoma. PMID: 21705376
  71. Patients with nonalcoholic steatohepatitis (NASH) had significantly higher levels of CK-18 and soluble Fas than patients in the "not NASH" group. PMID: 21145805
  72. the KRT8/KRT18 heterodimeric intermediary filaments of the cytoskeleton apparently are an essential component for the proper targeting of CFTR to the apical membrane in epithelial cells. PMID: 21548936
  73. speculated that chymotrypsin C might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression PMID: 21460362
  74. degradation of plectin induced by staurosporine-treatment in liver cells resulted in cytoskeleton disruption and induced morphological changes in these cells by affecting the expression and organization of cytokeratin 18. PMID: 21420381
  75. These findings reveal the potential for cytokeratin 18 to be used as a diagnostic marker for early detection of hepatosplenic schistosomiasis. PMID: 21357724
  76. Cervical cancer cells with diminished CK18 expression are more susceptible to cytokine-induced apoptosis, particularly in response to FasL treatment. PMID: 21119362
  77. evidence for increased total CK-18 serum and urine levels in chronic kidney disease patients PMID: 21195700
  78. caspase-cleaved epitope M30 serum levels are associated with survival in advanced gastric carcinoma patients PMID: 20362077
  79. The expression of CK-18-mRNA was significantly associated with clinical stages, tumor differentiation and lymphatic metastasis in oral squamous cell carcinoma. PMID: 19575986
  80. CK18 may play an important role in the progression of renal cell carcinoma PMID: 20372788
  81. The levels of urinary NMP22 and CK18 in the patients with transitional cell carcinoma of the bladder were significantly higher than those in the non-transitional cell carcinoma of the bladder. PMID: 19615282
  82. results indicate that keratin-18 could be regarded as a marker of liver damage and apoptosis in chronic hepatitis C. PMID: 20374042
  83. These data suggest that keratin 18 phosphorylation is a progression marker for chronic hepatitis b. PMID: 20334631
  84. Results reveal significantly increased preoperative values of circulating cytokeratin 18 fragment m65 in stage I and IIA colon cancer patients PMID: 19727975
  85. K18 attenuates estrogen receptor alpha-mediated signaling by sequestering LRP16 in cytoplasm. PMID: 20035625
  86. Over-expression of Akt1 increases K8/18 proteins. Akt2 up-regulates K18 expression by an increased mRNA stability. PMID: 20109457
  87. Serum contents of caspase-cleaved cytokeratin-18 and histone-associated-DNA-fragments were increased in patients with Chronic obstructive pulmonary disease (COPD), whereas anti-inflammatory soluble ST2 showed a peak in patients with COPD I&II. PMID: 19927353
  88. The crosstalk between angiogenesis, CK-18, and insulin resistance may play an important role in the onset and progression of non-alcoholic steatohepatitis. PMID: 19891019
  89. Serum levels of the apoptotic marker cytokeratin 18/M30 peak at 24 h after acute myocardial infarction and reflects the extent of coronary artery disease in this patient group. PMID: 19770641
  90. non-small cell lung carcinoma subtypes have specific patterns of desmoplakin 1 and 2 and cytokeratin 18 gene expression, protein content and biodistribution PMID: 11955647
  91. K18 mutation and its consequent keratin filament disruption predispose hepatocytes to Fas- but not TNF-mediated apoptotic injury. PMID: 12717381
  92. The expression of green fluorescent protein-tagged cytokeratin18 arg89cys induced aggregations and loss of the intermediate filament network composed of cytokeratins in liver-derived epithelial cells. PMID: 14648556
  93. CYK18 has a role in suppressing breast cancer progression PMID: 15102669
  94. The detection of CK18 neoepitope M30 in the serum might be a useful marker in tracing apoptotic epithelium in septic patients. PMID: 15316390
  95. Data show that the host intermediate filament protein cytokeratin 18 (CK18) is an interacting partner of EspF during enteropathogenic Escherichia coli (EPEC) pathogenesis. PMID: 15339273
  96. results lead to conclusion that, in colon epithelial cells, the expression level of the K18 gene is kept in check by a repression mechanism involving CtBP1, HDAC & BRCA1; mechanism is altered in SW613-S colon carcinoma cells that overexpress the K18 gene PMID: 15831101
  97. findings show that Ese-2 upregulates K18 gene expression through specific interactions within ETS binding sites in the regulatory first intron of the gene PMID: 15987600
  98. keratin 18 (K18) expression in tumor cells is associated with reduced invasiveness in vitro. PMID: 16046547
  99. CK18-mRNA is preserved through metaplasia, and metaplastic squamous cells differentiate with a decrease of CK18 and an increase of CK13 expression. PMID: 16836912
  100. Docetaxel induced increased levels of caspase-cleaved CK18 in serum from breast cancer patients, indicating apoptosis PMID: 17545523

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Involvement in disease Cirrhosis (CIRRH)
Subcellular Location Cytoplasm, perinuclear region, Nucleus, nucleolus
Protein Families Intermediate filament family
Tissue Specificity Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Database Links

HGNC: 6430

OMIM: 148070

KEGG: hsa:3875

STRING: 9606.ENSP00000373487

UniGene: Hs.406013

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