Human epididymal protein 4(HE4) ELISA kit

Code CSB-E12923h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name WAP four-disulfide core domain 2
Alternative Names dJ461P17.6 ELISA Kit; EDDM4 ELISA Kit; epididymal protein 4 ELISA Kit; Epididymal secretory protein E4 ELISA Kit; Epididymis specific whey acidic protein type four disulfide core ELISA Kit; HE 4 ELISA Kit; Major epididymis specific protein E4 ELISA Kit; Major epididymis-specific protein E4 ELISA Kit; MGC57529 ELISA Kit; Putative protease inhibitor WAP5 ELISA Kit; WAP 5 ELISA Kit; WAP domain containing protein HE4 ELISA Kit; WAP domain containing protein HE4 V4 ELISA Kit; WAP four disulfide core domain 2 ELISA Kit; WAP four disulfide core domain protein 2 ELISA Kit; WAP four-disulfide core domain protein 2 ELISA Kit; WAP5 ELISA Kit; WFDC 2 ELISA Kit; WFDC2 ELISA Kit; WFDC2_HUMAN ELISA Kit
Abbreviation WFDC2
Uniprot No. Q14508
Species Homo sapiens (Human)
Sample Types serum
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<20%        
Three samples of known concentration were tested in twenty assays to assess.      
To assess the linearity of the assay, samples were spiked with high concentrations of human HE4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 95    
Range % 85-102    
1:2 Average % 97    
Range % 85-105    
1:4 Average % 95    
Range % 83-101    
1:8 Average % 96    
Range % 84-107    
The recovery of human HE4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
Sample Type Average % Recovery Range    
Serum (n=5) 98 88-102    
EDTA plasma (n=4) 97 86-105    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
pmol/ml OD1 OD2 Average      
120 1.782 1.796 1.789      
48 0.941 0.952 0.947      
16.4 0.355 0.342 0.349      
6 0.131 0.129 0.130      
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews


Regarding your ELISA kit CSB-E12923h, could you please advise on the following:
1) Is the standard natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Does the standard include additive agent such as antiseptic or preservative? If included, what is the composition of additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

Thanks for your inquiry.
Standard is crude extract of native samples and it includes preservative.
Preservative is proclin 300(1:3000).
Stop solution is 4N sulfuric acid.
The kit doesn't contain azide, thimerosal, 2-ME.Pls let me know if you have any further questions. Thank you.

Target Data

Function Broad range protease inhibitor.
Gene References into Functions
  1. These results may be commented as HE4 expression rises in patients with HER2/neu amplification. PMID: 30004048
  2. Serum HE4 with its high specificity is useful in ruling out ovarian malignancy especially among the premenopausal women and Risk of Ovarian Malignancy Algorithm (ROMA)which is the combination of both serum CA 125 and HE4, appears to be an all-rounder with overall good sensitivity and specificity especially among the postmenopausal women. PMID: 30063463
  3. CA125 appears the most reliable biomarker for OC monitoring, whereas HE4 contributes additional information only in a minority of cases PMID: 30125544
  4. level of HE4 might predict chemoresistance and the possibility of ascites formation. The determination of the expression of HE4 alone or combined with CA125 levels in both serum and ascites in ovarian cancer patients with ascites may have important significance for guiding and improving the treatment PMID: 29903044
  5. Elevated serum HE4 in SLE patients is significantly associated with a higher risk of incidence for lupus nephritis (LN), and may be a useful predictor for developing LN. PMID: 29747124
  6. HE4 has the potential to be used as a novel clinical biomarker for evaluating kidney function and predicting renal fibrosis in kidney transplant recipients PMID: 29738696
  7. A panel of three tumor markers CYFRA 21.1, HE4 and ProGRP may play a role for discriminating LC from benign lung disease and subtyping as SCLC. PMID: 29729229
  8. Serum Smac expression level was significantly lower in the EAOC group than in the control group and benign ovarian tumor group (P< 0.05), while HE4 and CA125 expression levels were significantly higher in the EAOC group than the other two groups. PMID: 29226858
  9. This study demonstrated that altered expression of HE4 may involve in tumorigenesis in the uterine cervix. Our findings also suggested the presence of a correlation between HE4 expression and the invasive potential of uterine tumors. Therefore it may be thought that the tissue expression of HE4 can be used to differentiate high grade intraepithelial tumors from carcinomas. PMID: 28326512
  10. Use of HE4 instead of CA125 did not significantly improve diagnostic performance of risk of malignancy indices 1-4 in patients with an adnexal mass PMID: 29238719
  11. The significant effect of the normalization of the HE4 marker after therapy and 50% reduction of HE4 levels before interval cytoreductive surgery on PFS and OS confirmed that HE4 might be an independent prognostic factor of treatment response. PMID: 29584739
  12. HE4 could help with the differentiation of prognostically varied patient groups as well as with the decision-making process associated with the development of individual treatment plans PMID: 29786997
  13. Studied serum levels of Parkinson's disease-associated protein 7 (DJ-1) and human epididymis protein 4 (HE4) in endometrial cancer (EC) patients and healthy controls. The median serum concentrations of DJ-1 and HE4 were found to be significantly higher in EC patients compared to controls. PMID: 28374920
  14. HE4 is a biomarker for ovarian cancer recurrence [review] PMID: 28825178
  15. Numerous studies indicated that HE4 is able to promote cancer progression and drug resistance. These observations strongly suggest that HE4 may play a significant role in cancer recurrence through its bioactivity in the regulation of malignant behavior at the cellular level. Overall, available data shows that high serum HE4 levels correlate well with ovarian cancer recurrence. [review] PMID: 28382859
  16. WFCD2, which is up-regulated in ovarian cancer, can now for the first time be considered a regulator of tumor metastasis. PMID: 28679402
  17. High HE4 expression is associated with adnexal malignancy. PMID: 27116243
  18. Architect CA 125 II and HE4 values in Chinese women presenting with a pelvic mass PMID: 28549533
  19. Review/Meta-analysis: urine HE4 has diagnostic value in detecting ovarian cancer. PMID: 28039447
  20. HE4 seems to be superior to CA125 in the detection of recurrent disease. PMID: 29463191
  21. Data show that serum human epididymis protein 4 (HE4) levels were significantly positively associated with renal fibrosis in chronic kidney disease (CKD) patients. PMID: 27589683
  22. serum HE4 level was a marker of poor prognosis in lung cancer patients PMID: 28499820
  23. This review summarizes data regarding the detection and alteration of HE4 in urine samples collected from ovarian cancer patients and controls. PMID: 28117603
  24. HE4 is a prognostic marker in endometrial cancer PMID: 28401338
  25. HE4 levels correspond to clinical and FIGO stage of endometrial cancer PMID: 28476847
  26. HE4 is elevated less frequently than CA125 in benign adnexal tumors, regardless of age or menopausal status PMID: 27752776
  27. HE4 expression in biopsy material from endometrial hyperplasia can be monitored and regulated by progestin. Changes in the expression of HE4 during progestin therapy regimens can predict therapy response or indicate progestin resistance for medium- and low-risk endometrial hyperplasia. PMID: 27537387
  28. Serum HE4 levels were significantly elevated in children and adults with cystic fibrosis (CF) compared to controls. Abnormal but lower HE4 concentrations were found in cases of severe bronchitis, asthma, pneumonia, and bronchiectasis. HE4 mRNA levels were significantly upregulated in CF vs. non-CF airway biopsy specimens. PMID: 27105680
  29. Human epididymis protein 4 induces chemoresistance against anti-cancer drugs and activates the AKT and Erk pathways to enhance tumor survival. Human epididymis protein 4 expression in ovarian cancer tissue is associated with a worse prognosis for epithelial ovarian cancer patients. PMID: 27862665
  30. Study indicated that serum human epididymis protein 4 levels are closely associated with primary fallopian tube carcinoma and the outcome of surgical therapy and recurrence in Chinese patients. PMID: 27307153
  31. This study found that increased serum HE4 is independently associated with the development of Lupus Nephritis and Chronic Kidney Disease in Systemic Lupus Erythematosus patients. PMID: 27075413
  32. The present study displayed the most fundamental and full-scale data to show differentially expressed genes in response to HE4 PMID: 27430660
  33. We found the high specificity of HE4 and CA125 while differentiating ovarian benign diseases from epithelial ovarian cancer in postmenopausal women and the high sensitivity of CA125 in detecting epithelial ovarian cancer in premenopausal patients PMID: 27436085
  34. HE4 levels at primary diagnosis correlate with an increased risk of endometrial cancer recurrence, particularly in cases of endometrioid histotype, and they may help to recognize patients who may need a more intensive follow-up PMID: 26531723
  35. Overexpression of HE4 promotes collateral resistance to cisplatin and paclitaxel, and downregulation of HE4 partially reverses this chemoresistance. PMID: 27184254
  36. High Serum HE4 level is associated with epithelial ovarian cancer. PMID: 27629144
  37. HE4 and miR-21 may play an important role in the development and progression of ovarian cancer and they may serve as prognostic indicators in ovarian cancer PMID: 26733162
  38. HE4 had more potential than CA125. PMID: 27797265
  39. High serum HE-4 levels were associated with Endometrial Cancer. PMID: 27268621
  40. High HE4 expression is associated with Ovarian Tumors. PMID: 27268637
  41. High correlation with MRI parameters related to neoangiogenesis may indicate signaling pathway of HE4. PMID: 27192502
  42. The aim of the study was to establish whether preoperative serum levels of HE4 and CA125 could be a good predictor for lymphadenectomy in the early stage of endometrioid adenocarcinoma of the uterus. PMID: 26344910
  43. Data suggest that the combination of epididymis protein 4 (HE4) and CA125 may be recommended as potential biomarkers in the preliminary differential diagnosis of between epithelial ovarian cancer (EOC) and peritoneal tuberculosis (PTB). PMID: 26667154
  44. findings suggested that serum HE4 was a better biomarker in early lung cancer than other frequently-used biomarkers PMID: 26851650
  45. HE4 is a promising biomarker for the early diagnosis of epithelial ovarian cancer [review] PMID: 26220095
  46. serum level associated with risk in endometrioid adenocarcinoma PMID: 26223178
  47. HE4 alone and even more the combined analysis of CA125 and HE4 using risk of malignancy algorithm improve the diagnostic accuracy of adnexal masses, especially in early ovarian cancer. PMID: 21923475
  48. Data suggest that overexpressed human epididymis protein 4 (HE4) played an important role in ovarian cancer progression and metastasis and that HE4 has the potential to serve as therapeutic target for ovarian cancer. PMID: 26575020
  49. optimal cut-off values (HE4: 70pmol/l for all; CA125: 60U/ml for pre- and 35U/ml for postmenopausal women) could notably improve diagnostic performance in EOC detection in patients in southern China PMID: 26285075
  50. Results show that recombinant HE4 proteins are able to promote the proliferation of pancreatic and endometrial cancer cells suggesting that HE4 secreted from cancer cells may carry out paracrine and endocrine functions in cancer patients. PMID: 26497244
  51. Increased WFDC2 expression plays an important role in altering the estrogen pathway in ovarian cancer. PMID: 26928556
  52. Endometrial cancer patients with high HE4 expression have a poor prognosis. PMID: 26539494
  53. Data show that serum neuron-specific enolase, cytokeratin 19 fragment 21-1, pro-gastrin-releasing peptide, squamous cell carcinoma antigen, tissue inhibitor of metalloproteinase-1, and human epididymis protein 4 are not associated with brain metastases. PMID: 26730601
  54. In this report, we describe our use of capillary electrophoresis (CE) as the separation mechanism in the process of selecting DNA aptamers with affinity for the ovarian cancer biomarker HE4. PMID: 25863801
  55. Expression levels of ANXA2 and HE4 were closely related to the malignant biological behavior of endometrial carcinoma PMID: 26362938
  56. High serum HE4 level at diagnosis is an independent determinant of poor prognosis in NSCLC. PMID: 26030627
  57. Authors examined the serum concentration of human epididymis protein (HE4) in patients with benign gynecological diseases complicated with chronic renal deficiency PMID: 25867363
  58. Body mass index does not influence HE4 levels in serum. PMID: 25920696
  59. Serum HE4 measured during clinical follow-up may identify recurrent disease particularly in patients with endometrioid histology. PMID: 25655024
  60. HE4 was less elevated and more suitable as a biomarker than CA125 in chinese women with benign disease. PMID: 25834501
  61. HE4 overexpression induces resistance to antiestrogens in ovarian cancer. PMID: 24975515
  62. The HE4 marker concentrations in the peritoneal fluid are highly irrespective of the pathology observed in the female sexual organ. PMID: 24528554
  63. Our results suggest a potential role of HE4 in endometrial cancer stage prediction. PMID: 25577252
  64. HE4 protein played a promotive role in the proliferation and resistance to carboplatin-induced apoptosis in the ovarian cancer. PMID: 25354091
  65. High expression of WFDC2 was demonstrated for both, EOC and fallopian tube, as opposed to its low expression observed in normal ovaries suggesting that EOC is derived from fallopian tube rather than ovary PMID: 26117969
  66. we suggest He4 and ROMA score to be considered as routine preoperative assessment in the event that an ovarian mass is diagnosed and malignancy cannot be excluded. PMID: 25431767
  67. this study provides new evidence on promotive effects of the HE4 on gastric cancer progression and indicates that HE4 might be a promising prognostic factor for gastric cancer diagnosis. PMID: 25432133
  68. HE4 and annexin II binding interaction promoted ovarian cancer cell invasion. PMID: 25362534
  69. A HE4 level is a useful preoperative test for predicting the benign or malignant nature of pelvic masses.[review; meta-analysis] PMID: 25078339
  70. Median serum HE4 concentration was significantly higher in patients with epithelial ovarian cancer compared to patients with ovarian metastases of gastrointestinal origin. PMID: 25560808
  71. Measurement of combined HE4 and CA125 levels provides a more accurate method for epithelial ovarian cancer diagnosis. PMID: 25326813
  72. This study demonstrates that overexpression of the Lewis y antigen on HE4 promotes ovarian cancer cell invasion and metastasis, which is likely to be used as a target for the clinical treatment of ovarian cancer. PMID: 24998328
  73. Report presence of HE4 in vaginal secretions and its regulation in response to vaginal microbiota and to the concentrations of antimicrobial and proinflammatory mediators. PMID: 24023032
  74. Serum HE4 and CA125 as predictors of response and outcome during neoadjuvant chemotherapy of advanced high-grade serous ovarian cancer. PMID: 25190018
  75. the HE4 marker appears to be helpful in forecasting of optimal cytoreduction and possibly also of the prediction of response to platinum analogues used in first-line treatment of ovarian cancer. PMID: 25018782
  76. High serum HE4 levels are endometrial cancer. PMID: 24885319
  77. Human epididymis protein 4 might inhibit cell proliferation by regulating the mitogen-activated protein kinase and phosphoinositide 3-kinase/AKT signal transduction pathways in vitro. PMID: 24557433
  78. Data suggest the feasibility of using serum instead of bronchial aspiration fluid (BAF) samples for human epididymis 4 (HE-4) measurements in lung cancer cases. PMID: 24879624
  79. The modified cutoff value of CA125, HE4, RMI score and ROMA were 165.2 U/mL, 103.4 pM, 368.7, 28/54. PMID: 24716917
  80. High HE4 expression is associated with ovarian cancer relapse. PMID: 24217786
  81. Serum HE4 was not significantly elevated in patients with ovarian endometriosis. PMID: 25118488
  82. HE4 seems to be superior to Ca125 in terms of diagnostic performance of all premenopausal women PMID: 24771264
  83. The concentration of HE4 was noted to increase with age. PMID: 23464464
  84. Our findings suggest that HE4 values during first-line chemotherapy could predict chemotherapy response in epithelial ovarian cancer patients. PMID: 24748235
  85. These findings suggest that HE4 levels are greatly elevated in sera and tumor tissues of patients with SCLC and serum HE4 is a potential diagnostic marker for patients with SCLC. PMID: 25051914
  86. findings implicate increased HE4 expression as a molecular factor in ovarian cancer tumorigenesis PMID: 24389815
  87. HE4 variants exhibit tissue-specific expression, suggesting that each variant may exert distinct functions in normal and malignant cells. HE4 levels appear to correlate with EC patient survival in a variant-specific manner PMID: 24252907
  88. Reference intervals for serum HE4, CA125 and risk of ovarian malignancy algorithm in healthy Chinese female were established. PMID: 24012857
  89. High HE4 expression is associated with epithelial ovarian tumours. PMID: 24083699
  90. Serum HE4 may be used as a potential marker to differentiate lung cancer from pulmonary tuberculosis and healthy controls; higher levels of HE4 predict poor prognosis in non-small-cell lung cancer PMID: 24025389
  91. The analysis of published data suggests that HE4 is the most accurate and sensitive endometrial cancer marker identified to date; this new marker seems to have a good performance in diagnosis PMID: 24068567
  92. The serum HE4 level may have significance for the differential diagnosis of s LGSG and HGSG PMID: 24034113
  93. HE4 serum levels combined with CE CT imaging may improve the monitoring management of women affected by ovarian cancer. PMID: 23970060
  94. High HE4 expression is associated with endometrial cancer. PMID: 24403483
  95. HE4 contains Lewis y antigen PMID: 23894390
  96. High WFDC2 expression is associated with ovarian cancer. PMID: 23129262
  97. Serum levels of HE4 are related to both the stage and grade of tumor in patients with early stage endometrial neoplasms. PMID: 23640061
  98. HE4 gene plays an important role in regulating proliferation, apoptosis, migration, invasion of serous ovarian cancer cells by ERK pathway and protease system. PMID: 23608327
  99. HE4 significant correlation with decreased Overall Survival, Disease Free Survival and Progression Free Survival suggests its potential role as a novel prognostic marker for endometrial cancer PMID: 23096757
  100. Because of the strong effect of age on HE4, thresholds for HE4 are best defined for women of specific ages. PMID: 22962406

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Subcellular Location Secreted
Tissue Specificity Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described
Database Links

HGNC: 15939

OMIM: 617548

KEGG: hsa:10406

STRING: 9606.ENSP00000361761

UniGene: Hs.2719

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