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WFDC2 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA964489A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: HT29 whole cell lysate, Hela whole cell lysate, HT29 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate
    All lanes: HE4 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 25 kDa
    Observed band size: 25 kDa
  • Overlay Peak curve showing MCF-7 cells stained with CSB-RA964489A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Q14508
Target Names
WFDC2
Alternative Names
WAP four-disulfide core domain protein 2 (Epididymal secretory protein E4) (Major epididymis-specific protein E4) (Putative protease inhibitor WAP5), WFDC2, HE4 WAP5
Species Reactivity
Human
Immunogen
A synthesized peptide derived from Human WFDC2
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
17D2
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

WFDC2, also known as HE4 (human epididymis protein 4), is a secreted glycoprotein belonging to the WAP-type four-disulfide core domain family. Originally identified in epididymal epithelium, WFDC2 has gained significant attention as a serum biomarker in oncology research, particularly for ovarian cancer studies where it serves as a valuable complement to CA125 monitoring. Its putative protease inhibitor function and elevated expression in various malignancies make it a compelling target for researchers investigating tumor biology, biomarker development, and epithelial cell differentiation.

This recombinant monoclonal antibody, clone 17D2, offers the reproducibility and consistency that demanding research applications require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, you can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation data demonstrates reliable detection of WFDC2 across multiple human cell lines in Western blot applications, including HT29, HeLa, MCF7, A549, and HEPG2 whole cell lysates. The observed band at 25 kDa aligns with the predicted molecular weight, confirming specific target recognition. For flow cytometry applications, the antibody has been validated in MCF-7 cells using intracellular staining protocols, showing clear separation from isotype control, which supports its utility in quantitative single-cell analyses.

The antibody is supplied in a glycerol-containing buffer optimized for long-term storage stability and is compatible with ELISA, Western blot, and flow cytometry workflows. Whether you are characterizing WFDC2 expression patterns, validating cell models, or developing detection assays, this antibody provides a dependable tool for your research.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Customer Reviews and Q&A

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Target Background

Function
Broad range protease inhibitor.
Gene References into Functions
  1. These results may be commented as HE4 expression rises in patients with HER2/neu amplification. PMID: 30004048
  2. Serum HE4 with its high specificity is useful in ruling out ovarian malignancy especially among the premenopausal women and Risk of Ovarian Malignancy Algorithm (ROMA)which is the combination of both serum CA 125 and HE4, appears to be an all-rounder with overall good sensitivity and specificity especially among the postmenopausal women. PMID: 30063463
  3. CA125 appears the most reliable biomarker for OC monitoring, whereas HE4 contributes additional information only in a minority of cases PMID: 30125544
  4. level of HE4 might predict chemoresistance and the possibility of ascites formation. The determination of the expression of HE4 alone or combined with CA125 levels in both serum and ascites in ovarian cancer patients with ascites may have important significance for guiding and improving the treatment PMID: 29903044
  5. Elevated serum HE4 in SLE patients is significantly associated with a higher risk of incidence for lupus nephritis (LN), and may be a useful predictor for developing LN. PMID: 29747124
  6. HE4 has the potential to be used as a novel clinical biomarker for evaluating kidney function and predicting renal fibrosis in kidney transplant recipients PMID: 29738696
  7. A panel of three tumor markers CYFRA 21.1, HE4 and ProGRP may play a role for discriminating LC from benign lung disease and subtyping as SCLC. PMID: 29729229
  8. Serum Smac expression level was significantly lower in the EAOC group than in the control group and benign ovarian tumor group (P< 0.05), while HE4 and CA125 expression levels were significantly higher in the EAOC group than the other two groups. PMID: 29226858
  9. This study demonstrated that altered expression of HE4 may involve in tumorigenesis in the uterine cervix. Our findings also suggested the presence of a correlation between HE4 expression and the invasive potential of uterine tumors. Therefore it may be thought that the tissue expression of HE4 can be used to differentiate high grade intraepithelial tumors from carcinomas. PMID: 28326512
  10. Use of HE4 instead of CA125 did not significantly improve diagnostic performance of risk of malignancy indices 1-4 in patients with an adnexal mass PMID: 29238719
  11. The significant effect of the normalization of the HE4 marker after therapy and 50% reduction of HE4 levels before interval cytoreductive surgery on PFS and OS confirmed that HE4 might be an independent prognostic factor of treatment response. PMID: 29584739
  12. HE4 could help with the differentiation of prognostically varied patient groups as well as with the decision-making process associated with the development of individual treatment plans PMID: 29786997
  13. Studied serum levels of Parkinson's disease-associated protein 7 (DJ-1) and human epididymis protein 4 (HE4) in endometrial cancer (EC) patients and healthy controls. The median serum concentrations of DJ-1 and HE4 were found to be significantly higher in EC patients compared to controls. PMID: 28374920
  14. HE4 is a biomarker for ovarian cancer recurrence [review] PMID: 28825178
  15. Numerous studies indicated that HE4 is able to promote cancer progression and drug resistance. These observations strongly suggest that HE4 may play a significant role in cancer recurrence through its bioactivity in the regulation of malignant behavior at the cellular level. Overall, available data shows that high serum HE4 levels correlate well with ovarian cancer recurrence. [review] PMID: 28382859
  16. WFCD2, which is up-regulated in ovarian cancer, can now for the first time be considered a regulator of tumor metastasis. PMID: 28679402
  17. High HE4 expression is associated with adnexal malignancy. PMID: 27116243
  18. Architect CA 125 II and HE4 values in Chinese women presenting with a pelvic mass PMID: 28549533
  19. Review/Meta-analysis: urine HE4 has diagnostic value in detecting ovarian cancer. PMID: 28039447
  20. HE4 seems to be superior to CA125 in the detection of recurrent disease. PMID: 29463191
  21. Data show that serum human epididymis protein 4 (HE4) levels were significantly positively associated with renal fibrosis in chronic kidney disease (CKD) patients. PMID: 27589683
  22. serum HE4 level was a marker of poor prognosis in lung cancer patients PMID: 28499820
  23. This review summarizes data regarding the detection and alteration of HE4 in urine samples collected from ovarian cancer patients and controls. PMID: 28117603
  24. HE4 is a prognostic marker in endometrial cancer PMID: 28401338
  25. HE4 levels correspond to clinical and FIGO stage of endometrial cancer PMID: 28476847
  26. HE4 is elevated less frequently than CA125 in benign adnexal tumors, regardless of age or menopausal status PMID: 27752776
  27. HE4 expression in biopsy material from endometrial hyperplasia can be monitored and regulated by progestin. Changes in the expression of HE4 during progestin therapy regimens can predict therapy response or indicate progestin resistance for medium- and low-risk endometrial hyperplasia. PMID: 27537387
  28. Serum HE4 levels were significantly elevated in children and adults with cystic fibrosis (CF) compared to controls. Abnormal but lower HE4 concentrations were found in cases of severe bronchitis, asthma, pneumonia, and bronchiectasis. HE4 mRNA levels were significantly upregulated in CF vs. non-CF airway biopsy specimens. PMID: 27105680
  29. Human epididymis protein 4 induces chemoresistance against anti-cancer drugs and activates the AKT and Erk pathways to enhance tumor survival. Human epididymis protein 4 expression in ovarian cancer tissue is associated with a worse prognosis for epithelial ovarian cancer patients. PMID: 27862665
  30. Study indicated that serum human epididymis protein 4 levels are closely associated with primary fallopian tube carcinoma and the outcome of surgical therapy and recurrence in Chinese patients. PMID: 27307153
  31. This study found that increased serum HE4 is independently associated with the development of Lupus Nephritis and Chronic Kidney Disease in Systemic Lupus Erythematosus patients. PMID: 27075413
  32. The present study displayed the most fundamental and full-scale data to show differentially expressed genes in response to HE4 PMID: 27430660
  33. We found the high specificity of HE4 and CA125 while differentiating ovarian benign diseases from epithelial ovarian cancer in postmenopausal women and the high sensitivity of CA125 in detecting epithelial ovarian cancer in premenopausal patients PMID: 27436085
  34. HE4 levels at primary diagnosis correlate with an increased risk of endometrial cancer recurrence, particularly in cases of endometrioid histotype, and they may help to recognize patients who may need a more intensive follow-up PMID: 26531723
  35. Overexpression of HE4 promotes collateral resistance to cisplatin and paclitaxel, and downregulation of HE4 partially reverses this chemoresistance. PMID: 27184254
  36. High Serum HE4 level is associated with epithelial ovarian cancer. PMID: 27629144
  37. HE4 and miR-21 may play an important role in the development and progression of ovarian cancer and they may serve as prognostic indicators in ovarian cancer PMID: 26733162
  38. HE4 had more potential than CA125. PMID: 27797265
  39. High serum HE-4 levels were associated with Endometrial Cancer. PMID: 27268621
  40. High HE4 expression is associated with Ovarian Tumors. PMID: 27268637
  41. High correlation with MRI parameters related to neoangiogenesis may indicate signaling pathway of HE4. PMID: 27192502
  42. The aim of the study was to establish whether preoperative serum levels of HE4 and CA125 could be a good predictor for lymphadenectomy in the early stage of endometrioid adenocarcinoma of the uterus. PMID: 26344910
  43. Data suggest that the combination of epididymis protein 4 (HE4) and CA125 may be recommended as potential biomarkers in the preliminary differential diagnosis of between epithelial ovarian cancer (EOC) and peritoneal tuberculosis (PTB). PMID: 26667154
  44. findings suggested that serum HE4 was a better biomarker in early lung cancer than other frequently-used biomarkers PMID: 26851650
  45. HE4 is a promising biomarker for the early diagnosis of epithelial ovarian cancer [review] PMID: 26220095
  46. serum level associated with risk in endometrioid adenocarcinoma PMID: 26223178
  47. HE4 alone and even more the combined analysis of CA125 and HE4 using risk of malignancy algorithm improve the diagnostic accuracy of adnexal masses, especially in early ovarian cancer. PMID: 21923475
  48. Data suggest that overexpressed human epididymis protein 4 (HE4) played an important role in ovarian cancer progression and metastasis and that HE4 has the potential to serve as therapeutic target for ovarian cancer. PMID: 26575020
  49. optimal cut-off values (HE4: 70pmol/l for all; CA125: 60U/ml for pre- and 35U/ml for postmenopausal women) could notably improve diagnostic performance in EOC detection in patients in southern China PMID: 26285075
  50. Results show that recombinant HE4 proteins are able to promote the proliferation of pancreatic and endometrial cancer cells suggesting that HE4 secreted from cancer cells may carry out paracrine and endocrine functions in cancer patients. PMID: 26497244

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Subcellular Location
Secreted.
Tissue Specificity
Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described
Database Links

HGNC: 15939

OMIM: 617548

KEGG: hsa:10406

STRING: 9606.ENSP00000361761

UniGene: Hs.2719

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