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Human glypican 5 (GPC5) ELISA kit

Human glypican 5 (GPC5) ELISA kit

Specification
Target

Code

CSB-EL009707HU

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P78333

Lead Time

7-14 working days

Abbreviation

GPC5

Target Name

glypican 5

Alias

, bA93M14.1|glypican proteoglycan 5

Species

Homo sapiens (Human)

Sample Types

serum, plasma, ascitic fluid

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

HGNC

4453

RGD

1308835

MGI

1194894

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GPC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	97
	Range %	92-102
1:4	Average %	100
	Range %	94-107
1:8	Average %	108
	Range %	103-114

Recovery

The recovery of human GPC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-106
EDTA plasma (n=4)	95	89-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.051	2.012	2.032	1.847
10	1.788	1.798	1.793	1.608
5	1.374	1.368	1.371	1.186
2.5	0.842	0.856	0.849	0.664
1.25	0.494	0.485	0.490	0.305
0.625	0.315	0.326	0.321	0.136
0.312	0.279	0.281	0.280	0.095
0	0.180	0.190	0.185

References

Elevated plasma glypicans are associated with organ failure in patients with infection Fisher J, et al,Intensive Care Medicine Experimental,January 7

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Function	Cell surface proteoglycan that bears heparan sulfate.
Subcellular Location	Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, SUBCELLULAR LOCATION: Secreted glypican-5: Secreted, extracellular space
Protein Families	Glypican family
Tissue Specificity	In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.
Database Links	HGNC: 4453 OMIM: 602446 KEGG: hsa:2262 STRING: 9606.ENSP00000366267 UniGene: Hs.655675

Related Products

GPC5 Antibody

GPC5 Protein

GPC5 Antibodies

GPC5 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA009707GA01HU	GPC5 Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA008249	GPC5 Antibody	Human,Mouse,Rat	WB,ELISA

GPC5 Proteins

GPC5 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP009707HU CSB-EP009707HU CSB-BP009707HU CSB-MP009707HU	Recombinant Human Glypican-5(GPC5)	Yeast E.coli Baculovirus Mammalian cell

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