Product Details

Target Name

glypican 5

Alternative Names

bA93M14.1 ELISA Kit; glypican 5 ELISA Kit; Glypican 5 precursor ELISA Kit; glypican proteoglycan 5 ELISA Kit; Glypican-5 ELISA Kit; GPC5 ELISA Kit; GPC5_HUMAN ELISA Kit; Secreted glypican-5 ELISA Kit

Abbreviation

GPC5

Uniprot No.

P78333

Species

Homo sapiens (Human)

Sample Types

serum, plasma, ascitic fluid

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GPC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	97
	Range %	92-102
1:4	Average %	100
	Range %	94-107
1:8	Average %	108
	Range %	103-114

Recovery

The recovery of human GPC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-106
EDTA plasma (n=4)	95	89-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.051	2.012	2.032	1.847
10	1.788	1.798	1.793	1.608
5	1.374	1.368	1.371	1.186
2.5	0.842	0.856	0.849	0.664
1.25	0.494	0.485	0.490	0.305
0.625	0.315	0.326	0.321	0.136
0.312	0.279	0.281	0.280	0.095
0	0.180	0.190	0.185

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human GPC5 ELISA Kit was designed for the quantitative measurement of Human GPC5 protein in serum, plasma, ascitic fluid. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Background

Function
(From Uniprot)

Cell surface proteoglycan that bears heparan sulfate.

Gene References into Functions

Our results showed that GPC5 was lowly expressed in PCa cell lines. PMID: 28893348
Taken together, our results suggest GPC5 as a tumor suppressor in PDAC and its expression is possibly regulated by miR-4295. Our study indicates that the miR-4295/GPC5 axis may play an important role in the pathogenesis of PADC and has potential applications for the development of PDAC therapy. PMID: 29407175
Suggest that GPC5 is able to suppress the lung adenocarcinoma metastasis by competitively binding to Wnt3a and inactivating the Wnt/beta-catenin signaling pathway. PMID: 27806326
GPC5 is a novel epigenetically silenced tumor suppressor, which inhibits tumor growth by suppressing Wnt/beta-catenin signaling in lung adenocarcinoma. PMID: 27157618
GPC5 protein expression showed a close correlation with the tumorigenesis and tumor progression of prostate cancer, and that might be applied as a novel biomarker for the prediction of diagnosis and prognosis of prostate cancer. PMID: 26631038
These results suggest that miR-297 acted as an oncogenic miRNA, partly by targeting GPC5, adenocarcinoma of the lung. PMID: 27554041
Results suggest a potential association between CRP and GPC5 variants with lung cancer risk; variation in GKN1 is associated with chemotherapy response in the Chinese Han population. PMID: 25999661
miR-709 may positively regulate invasion and metastasis of hepatocellular carcinoma through targeting GPC5. PMID: 25818666
gene variants in IRF5, IRF8 and GPC5 were not associated with risk of relapse or disease progression in multiple sclerosis PMID: 24943672
GPC5, a tumor suppressor, is regulated by miR-620 in lung adenocarcinoma. PMID: 24682381
Variants in the 50-upstream region of GPC5 confer risk of lung cancer in never smokers PMID: 24456789
These results indicate that GPC5 polymorphisms would be useful genetic indicators for Inflammatory demyelinating diseasess, including neuromyelitis optica and multiple sclerosis . PMID: 24135429
The IL7R, TNFRSF1A, and GPC5 polymorphisms tended to be associated with having a second event of Multiple sclerosis within a year. PMID: 24130709
Shh binds to HSPG co-receptors containing a glypican 5 core and 2-O-sulfo-iduronic acid to promote neural precursor proliferation. PMID: 23867465
Data suggest that glypican-5 (GPC5) is a metastasis suppressor gene in non-small cell lung cancer (NSCLC) and may be a potential biomarker that predicts NSCLC metastasis. PMID: 23962560
GPC5 is a gene required for normal neural tube development. PMID: 23223018
findings suggested that SNP rs2352028 in GPC5 might confer a slight risk to lung cancer/adenocarcinoma PMID: 22236185
GPC5 stimulates Hh signaling by facilitating/stabilizing the interaction between Hh and Ptc1. PMID: 21339334
GPC5 is a novel genetic locus protective against sudden cardiac arrest PMID: 20360844
This study supports that multiple sclerosis susceptibility at 13q31-32 may localize to the Glypican-5 gene. PMID: 20692050
No association between GPC5rs2352028 variant and lung cancer in never smokers in meta-analysis of 7 genome-wide association studies under the random effects model. PMID: 20688270
GPC5 polymorphism associated with susceptibility to lung cancer in never smokers PMID: 20304703
GPC5 is a possible target for the 13q31-q32 amplification detected in lymphoma cell lines. PMID: 12721791
Independent replication confirms GPC5 as a genetic risk factor for multiple sclerosis. PMID: 19010793
ZIC2, SPRY2, and GPC5 genes are candidate genes suspected to explain the malformations associated with cerebral anomalies in the hypothesis of a contiguous gene syndrome in 13q deletion syndrome PMID: 19022413
Confirm the association of polymorphisms within GPC5 with response to interferon-beta therapy in patients with multiple sclerosis. PMID: 19556317
Genome-wide association study of gene-disease association and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18195134

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.; [Secreted glypican-5]: Secreted, extracellular space.

Protein Families

Glypican family

Tissue Specificity

In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.

Database Links

HGNC: 4453

OMIM: 602446

KEGG: hsa:2262

STRING: 9606.ENSP00000366267

UniGene: Hs.655675

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Human glypican 5 (GPC5) ELISA kit

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Target Background