Human glypican 5 (GPC5) ELISA kit

Code CSB-EL009707HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P78333
Lead Time 7-14 working days
Abbreviation GPC5
Target Name glypican 5
Alias , bA93M14.1|glypican proteoglycan 5
Species Homo sapiens (Human)
Sample Types serum, plasma, ascitic fluid
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
HGNC 4453
RGD 1308835
MGI 1194894
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GPC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 88-97
1:2 Average % 97
Range % 92-102
1:4 Average % 100
Range % 94-107
1:8 Average % 108
Range % 103-114
Recovery
The recovery of human GPC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 95-106
EDTA plasma (n=4) 95 89-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.051 2.012 2.032 1.847
10 1.788 1.798 1.793 1.608
5 1.374 1.368 1.371 1.186
2.5 0.842 0.856 0.849 0.664
1.25 0.494 0.485 0.490 0.305
0.625 0.315 0.326 0.321 0.136
0.312 0.279 0.281 0.280 0.095
0 0.180 0.190 0.185  
References
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Function Cell surface proteoglycan that bears heparan sulfate.
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, SUBCELLULAR LOCATION: Secreted glypican-5: Secreted, extracellular space
Protein Families Glypican family
Tissue Specificity In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.
Database Links

HGNC: 4453

OMIM: 602446

KEGG: hsa:2262

STRING: 9606.ENSP00000366267

UniGene: Hs.655675

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