| Description |
The Human high sensitivity C-Reactive Protein (hs-CRP) ELISA Kit is engineered for accurate measurement of hs-CRP levels from samples, including serum, plasma, cell culture supernates, and tissue homogenates. It uses the direct Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the hs-CRP content in the sample. Hs-CRP is a sensitive and stable biomarker of inflammation that strongly and independently predicts future cardiovascular disease (CVD), including myocardial infarction, ischemic stroke, and vascular death. Wenying Li etc. demonstrated that elevated serum hs-CRP levels are related to the severity of erectile dysfunction (ED) and an increased ED risk in a Chinese male population. These findings suggest that hs-CRP may be a valuable inflammatory marker for estimating ED risk and may play an important role in the etiology of ED.
The kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. See more details through the product instructions. |
| Target Name |
high sensitivity C-Reactive Protein,hs-CRP |
| Alternative Names |
Pentraxin 1, short ELISA Kit; C reactive protein ELISA Kit; C reactive protein pentraxin related ELISA Kit; C-reactive protein(1-205) ELISA Kit; CRP ELISA Kit; CRP_HUMAN ELISA Kit; MGC88244 ELISA Kit; Pentraxin 1 ELISA Kit; PTX 1 ELISA Kit; PTX1 ELISA Kit |
| Abbreviation |
hs-CRP |
| Uniprot No. |
P02741 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others
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| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:400 |
Average % |
104 |
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| Range % |
100-108 |
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| 1:800 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:1600 |
Average % |
99 |
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| Range % |
93-104 |
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| Recovery |
| The recovery of human CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-96 |
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| EDTA plasma (n=4) |
99 |
95-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.859 |
2.876 |
2.868 |
2.760 |
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| 20 |
2.261 |
2.234 |
2.248 |
2.140 |
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| 10 |
1.436 |
1.412 |
1.424 |
1.316 |
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| 5 |
0.744 |
0.723 |
0.734 |
0.626 |
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| 2.5 |
0.408 |
0.422 |
0.415 |
0.307 |
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| 1.25 |
0.251 |
0.237 |
0.244 |
0.136 |
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| 0.625 |
0.167 |
0.158 |
0.163 |
0.055 |
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| 0 |
0.106 |
0.110 |
0.108 |
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| Materials provided |
- A micro ELISA plate--- The 96-well plate has been pre-coated with an anti-human hs-CRP antibody.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated hs-CRP antibody (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial HRP-avidin Diluent (20 ml/bottle) ---Dilute HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the standard and sample solutions.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
