Human high sensitivity C-Reactive Protein,hs-CRP ELISA Kit

Instructions
Code CSB-E08617h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

The Human high sensitivity C-Reactive Protein (hs-CRP) ELISA Kit is engineered for accurate measurement of hs-CRP levels from samples, including serum, plasma, cell culture supernates, and tissue homogenates. It uses the direct Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the hs-CRP content in the sample. Hs-CRP is a sensitive and stable biomarker of inflammation that strongly and independently predicts future cardiovascular disease (CVD), including myocardial infarction, ischemic stroke, and vascular death. Wenying Li etc. demonstrated that elevated serum hs-CRP levels are related to the severity of erectile dysfunction (ED) and an increased ED risk in a Chinese male population. These findings suggest that hs-CRP may be a valuable inflammatory marker for estimating ED risk and may play an important role in the etiology of ED.

The kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. See more details through the product instructions.

Target Name high sensitivity C-Reactive Protein,hs-CRP
Alternative Names Pentraxin 1, short ELISA Kit; C reactive protein ELISA Kit; C reactive protein pentraxin related ELISA Kit; C-reactive protein(1-205) ELISA Kit; CRP ELISA Kit; CRP_HUMAN ELISA Kit; MGC88244 ELISA Kit; Pentraxin 1 ELISA Kit; PTX 1 ELISA Kit; PTX1 ELISA Kit
Abbreviation hs-CRP
Uniprot No. P02741
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 95  
Range % 91-99  
1:400 Average % 104  
Range % 100-108  
1:800 Average % 93  
Range % 89-97  
1:1600 Average % 99  
Range % 93-104  
Recovery
The recovery of human CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-96  
EDTA plasma (n=4) 99 95-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.859 2.876 2.868 2.760  
20 2.261 2.234 2.248 2.140  
10 1.436 1.412 1.424 1.316  
5 0.744 0.723 0.734 0.626  
2.5 0.408 0.422 0.415 0.307  
1.25 0.251 0.237 0.244 0.136  
0.625 0.167 0.158 0.163 0.055  
0 0.106 0.110 0.108    
Materials provided
  • A micro ELISA plate--- The 96-well plate has been pre-coated with an anti-human hs-CRP antibody.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial HRP-conjugated hs-CRP antibody (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial HRP-avidin Diluent (20 ml/bottle) ---Dilute HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the standard and sample solutions.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Displays several functions associated with host defense
Gene References into Functions
  1. gemcabene decreases CRP by C/EBP-delta and NF-kappaB-mediated transcriptional mechanism and suppresses IL-6 and IL-1beta-induced CRP production. PMID: 29644527
  2. Combinative index (arterial lactate, NT-proBNP and CRP) could serve as a valuable predictor for the 28-day mortality rate in patients with sepsis. PMID: 30158031
  3. CRP rs3093061, rs1130864, rs1205, and rs3091244 polymorphisms are associated with decreased risk of developing Systemic Lupus erythematosus PMID: 29556849
  4. The study showed that higher CRP and lower T3 levels were associated with stroke severity on admission. Functional outcome is likely secondary to stroke severity but functional outcome at discharge was associated with higher CRP levels. PMID: 30056003
  5. These findings reveal that the CRP- and PTX3-binding characteristics of FHL-1 differ from those of FH, likely underpinning independent immune regulatory functions in the context of the human retina. PMID: 29374201
  6. Short-term changes in daily movement behaviour can affect salivary CRP, a marker of systemic inflammation. PMID: 29499122
  7. M1 macrophages were characterized by elevated CRP mRNA expression (by 67%), CRP protein levels (by 108%), and upregulation of NFkappaB activation PMID: 29687414
  8. Levels of CRP and PCT in CSF did not present greater accuracy in differentiating bacterial from viral meningitis compared to serum levels. PMID: 30143494
  9. elevated serum C-reactive protein (CRP) is a well-known proxy for infections/immune abnormalities. Our results show elevated C-reactive protein levels to be associated with an increase in risk for schizophrenia. PMID: 29306175
  10. High CRP expression is associated with Nonalcoholic fatty liver disease. PMID: 29402657
  11. High C-reactive protein expression is associated with impaired glucose tolerance. PMID: 29078256
  12. Findings indicate that childhood infections do not have an independent, lasting effect on circulating inflammatory marker levels subsequently in childhood; however, elevated inflammatory markers may be harmful for intellectual development/function. PMID: 29198208
  13. maternal serum level has predictive value for chorioamnionitis PMID: 29430983
  14. serum level elevated in preterm labor leading to preterm birth PMID: 29426269
  15. Among healthy women attempting pregnancy after one or two pregnancy losses, elevated C-reactive protein is associated with reduced fecundability. PMID: 29317123
  16. Suggest a close association between CRP and metabolic syndrome, while the association between the dietary inflammatory index and MetS was limited. PMID: 29954070
  17. pre-procedural serum CRP could be a valuable predictor of global cardiovascular risk. PMID: 28128312
  18. Data suggest that serum CRP levels show positive association with ChildTurcottePugh scores in subjects with liver cirrhosis; GSH levels show negative association with ChildTurcottePugh scores; thus, CRP and GSH appear to exert synergistic effects in association with severity of liver cirrhosis. This cross-sectional study was conducted in Taiwan. (GSH = glutathione) PMID: 29861471
  19. Study shows for the first time that CRP activates signaling of integrin alpha2 via binding to integrin alpha2 and Fcgamma receptor I in breast cells and MDA-MB-231 triple-negative human breast cancer cells. PMID: 28846105
  20. This meta-analysis suggests that C-reactive protein/albumin ratio (CAR)may be a potential prognostic marker in solid cancers PMID: 28128229
  21. The results of the study suggest that the levels of C-reactive protein and tumor necrosis factor-alpha are important diagnostic markers of inflammation in patients with chronic pancreatitis and type 2 diabetes mellitus. PMID: 30280549
  22. Elevated serum C-reactive protein could be a bacterial infection predictor in decompensated cirrhosis. PMID: 29384797
  23. The results showed that the MI-based bio-sensing system had high selectivity and sensitivity for detection of Cardiac biomarkers . Compared with the control region, ultrasensitive detections of CRP and Mb were accomplished with the detection limits of 1.0 pg/mL and 0.1 pg/mL, respectively. PMID: 29857573
  24. Data suggest that C-reactive protein (CRP) may play an important role in the development of tongue squamous cell carcinoma (TSCC). PMID: 29385406
  25. For WBC, N (%), and ESR, both groups showed similar patterns of temporal values 14 days after surgery regardless of preoperative CRP level. Our findings could be used as guidelines for patient discharge and during the follow-up period after surgery. PMID: 29854731
  26. The neutrophil/lymphocyte ratio is a better addition to C-reactive protein than CD64 index as a marker for infection in COPD. PMID: 28185443
  27. This retrospective cohort study aimed to identify the early postoperative kinetics of C-reactive protein (CRP) and procalcitonin (PCT) in children undergoing tetralogy of Fallot (ToF) correction. PMID: 29111402
  28. After beta-blocker treatment, hs-CRP levels are associated with functional capacity in heart failure patients. PMID: 29396658
  29. Hypertensive patients with coronary tortuosity have a higher serum hs-CRP level concentration and have a higher incidence of lacunar infarction than hypertensive patients without coronary tortuosity. PMID: 29436255
  30. High CRP expression is associated with non-small cell lung cancer. PMID: 29549910
  31. T allele of rs1205 in CRP gene was protective against Takayasu arteritis in our patients. CC genotype was associated with involvement of coronary artery and ascending aorta. A trend toward a blunted CRP response seen in patients. PMID: 29024426
  32. Serum C-reactive protein (and COMP) levels were both associated with the incidence of knee osteoarthritis. PMID: 29351749
  33. coexistence of both higher HCY and higher hs-CRP in the acute phase of ischemic stroke were associated with subsequent PSD, independently of established conventional risk factors PMID: 29325799
  34. Data suggest that C-reactive-protein (CRP) concentration is weakly associated with global DNA methylation level, and this association was more clearly in individuals with the minor allele of the 5,10-methylenetetrahydrofolate reductase (MTHFR)missense SNP rs1801133. PMID: 29439678
  35. A longitudinal association between adolescent CRP levels and adult schizophrenia diagnosis indicates a potentially important role of inflammation in the pathogenesis of the illness PMID: 27622678
  36. rs1205 genetic variability in the CRP gene determines the CRP levels in sepsis of different severities PMID: 29379005
  37. CRP levels predict the development of anastomotic leak and postoperative mortality after colorectal cancer surgery. PMID: 28132052
  38. Uremia patients receiving maintenance hemodialysis with hospitalacquired infection had increased serum inflammatory factors and high throughput hemodialysis significantly decreased CRP, IL2 and TNFalpha levels in the serum, suggesting that high throughput hemodialysis may be beneficial for the prevention of the infections in uremia patients. PMID: 29257244
  39. High CRP expression is associated with cardiovascular disease. PMID: 28724840
  40. The results of this meta-analysis suggest that increased CRP expression indicates a poorer prognosis in patients with bone neoplasms. PMID: 29668751
  41. C-reactive protein (CRP) recognized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG) in the supported 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) monolayers without calcium at pH 7.4 and 5.5. PMID: 29172154
  42. Used Ultra Performance Liquid Chromatography-Ultra Definition Mass Spectrometry-based proteomics to compare serum samples from 19 colorectal cancer (CRC) patients. The samples were first analyzed after dividing them into two categories based on CRP, CRP <30 and CRP >30, and the same samples were analyzed again after being divided into the categories short and long 5-year survival. PMID: 29630649
  43. CRP bound to surface CD32 (also known as FcgammaRII) on myeloma cells, which activated a pathway mediated by the kinase p38 MAPK and the transcription factor Twist that enhanced the cells' secretion of osteolytic cytokines. PMID: 29233917
  44. high-sensitivity C-reactive protein could have a role in recurrent stroke and vascular events in patients treated with statins PMID: 28302952
  45. Procalcitonin and CRP serum levels are associated with the appearance of intra-abdominal infection after colorectal cancer surgery. PMID: 28918433
  46. C-reactive protein polymorphisms and DNA methylation of the AIM2 gene influence associations between trauma exposure, posttraumatic stress disorder, and C-reactive protein. PMID: 28867284
  47. Elevated CRP level is correlated with poor prognosis in patients with metastatic renal cell carcinoma receiving tyrosine kinase inhibitors treatment. (Meta-analysis) PMID: 29080691
  48. The study reveals that CRP might be a clear cell renal cell carcinoma development promoter. PMID: 28923830
  49. CRP distinguished sepsis and non-septic ICU patients. PMID: 28898742
  50. Suggest that hs-CRP may be a marker of comorbidities associated with worse health status in myocardial infarction. PMID: 28946036

Show More

Hide All

Subcellular Location Secreted
Protein Families Pentraxin family
Tissue Specificity Found in plasma.
Database Links

HGNC: 2367

OMIM: 123260

KEGG: hsa:1401

STRING: 9606.ENSP00000255030

UniGene: Hs.709456

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1