Human mucin-5AC (MUC5AC) ELISA kit

Instructions
Code CSB-E10109h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

The product CSB-E10109h is a ready-to-use ELISA kit for the quantification of mucin-5AC (MUC5AC) in human serum, plasma, cell culture supernates, or urine. This assay employs the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction to achieve the measurement. The solution develops in proportion to the amount of MUC5AC in the sample. And the color intensity can be measured at 450 nm through a microplate reader. The kit has undergone rigorous quality validation in many aspects, including sensitivity, specificity, precision, recovery, linearity, and consistency between batches.

MUC5AC is a gel-forming mucin with multiple domains containing a heavily O-glycosylated apoprotein core and extensive intramolecular disulfide bonds between the cysteine-rich amino and carboxy-terminal domains. It exerts versatile effects in many biological processes, including barrier functions to epithelial cells, interactions between host and pathogen, immune cell attraction to sites of premalignant or malignant lesions, and tumor progression in a context-dependent manner. Differential expression, glycosylation, and localization of MUC5AC have been linked to a variety of benign and malignant pathologies.

Target Name mucin-5AC
Alternative Names MUC5AC ELISA Kit; MUC5 ELISA Kit; Mucin-5AC ELISA Kit; MUC-5AC ELISA Kit; Gastric mucin ELISA Kit; Major airway glycoprotein ELISA Kit; Mucin-5 subtype AC ELISA Kit; tracheobronchial ELISA Kit; Tracheobronchial mucin ELISA Kit; TBM ELISA Kit
Abbreviation MUC5AC
Uniprot No. P98088
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, urine
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 87-99  
1:2 Average % 94  
Range % 87-96  
1:4 Average % 98  
Range % 94-106  
1:8 Average % 90  
Range % 86-97  
Recovery
The recovery of human MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 95-93  
EDTA plasma (n=4) 94 91-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
1000 2.452 2.563 2.508 2.375  
500 1.949 1.964 1.957 1.824  
250 1.358 1.431 1.395 1.262  
125 0.812 0.832 0.822 0.689  
62.5 0.536 0.576 0.556 0.423  
31.2 0.342 0.356 0.349 0.216  
15.6 0.241 0.249 0.245 0.112  
0 0.132 0.134 0.133    
Materials provided
  • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human MUC5AC antibody.This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labled MUC5AC antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
Gene References into Functions
  1. S100A12 activates NLPR3 inflammasomes to induce MUC5AC production in airway epithelial cells. ATP induces MUC5AC production in a mechanistically similar mode to S100A12. PMID: 29906464
  2. The results of the present study indicate that Munc132 may be an essential regulator of basal MUC5AC exocytosis, while Munc134 appears to be a Munc13 protein subtype that may to be sensitive to hNE stimulation during airway MUC5AC hypersecretion. PMID: 29767240
  3. resistin induced MUC5AC and MUC5B expression via activation of different signaling pathways in human airway epithelial cells. PMID: 29604272
  4. the splice switch to DeltaC-ZIP2 as well as decreased expression of other ZIPs caused zinc deficiency, which is sufficient for induction of MUC5AC. PMID: 29289532
  5. this paper shows that IL-1beta upregulates Muc5ac expression via NF-kappaB-induced HIF-1alpha in asthma PMID: 29031476
  6. Data suggest that secreted IL-6 regulates CLB2.0-induced MUC5AC and MUC1 expression via ROS-mediated downregulation of claudin-1 expression to maintain mucus homeostasis in the airway. PMID: 28946937
  7. Helicobacter pylori (H. pylori)-CagA significantly upregulated MUC5AC, MUC2, and MUC5B expression in AGS cells, but did not affect E-cadherin and MUC6 expression. PMID: 29869461
  8. Oct-4 expression and the reduction of MUC5AC expression may be involved in the progression and an unfavorable prognosis of GC PMID: 28762513
  9. smoking might decrease tear secretion, goblet cell density and tear MUC5AC concentration. PMID: 27297822
  10. High-Mobility Group Box 1 Upregulates MUC5AC and MUC5B Expression in Primary Airway Epithelial Cells PMID: 29286856
  11. Cyclic pressure did not induce MUC5AC secretion in the airway mucus epithelium via Ca(2+)-dependent ATP release, and nearly all Ca(2+) was provided by stored intracellular Ca(2). PMID: 27919041
  12. IL-33 induced MUC5AC mRNA and MUC5AC protein, and also goblet cell hyperplasia at air liquid interface culture in human nasal epithelial cells. In addition to that, IL-33 induced MUC5B and FOXA3, and reduces FOXJmRNA. PMID: 27776277
  13. MUC5AC has pathologic roles in mechanisms of allergen-induced airway hyperresponsiveness, mucous metaplasia, and airway mucus plugging. PMID: 27845589
  14. Leptin knockdown suppressed MUC5AC production and secretion induced by IL-13 in human bronchial epithelial cells. PMID: 28942146
  15. The results suggest that the analysis of expression of MUC5AC and TFF1 may be useful for differentiating sessile serrated adenomas/polyps (SSA/Ps) from hyperplastic polyps (HPs). PMID: 28430953
  16. The Egr-1 is essential for CSE-induced MUC5AC production in HBE cells likely through interaction with and modulation of AP-1, and re-emphasize targeting Egr-1 as a novel therapeutic strategy for COPD. PMID: 28602698
  17. Mucus plugs from individuals with fatal asthma are heterogeneous gels with distinct MUC5AC- and MUC5B-containing domains. Tethering of MUC5AC-containing domains to the epithelium causes mucostasis and likely represents a major cause of mucus plugging in asthma. PMID: 27183390
  18. The only individual marker that was found to have direct and strong correlation with the clinical outcome of ampullary carcinoma was MUC5AC (not used in the Ang or Chang panels), with statistically significant survival differences found with various cutoffs tested (for 20% cutoff, 5-y survival, 68% vs. 31%; P=0.0002). In addition, MUC5AC significantly stratified the histologically pancreatobiliary and intestinal cases. PMID: 28505002
  19. MUC5AC interacts with integrin beta4 that mediates phosphorylation of FAK at Y397 leading to lung cancer cell migration. PMID: 26751774
  20. Results show that the glycoforms of MUC5AC in mucinous cysts of the pancreas display two unusual motifs, terminal alphaGlcNAc and terminal betaGlcNAc. PMID: 27992432
  21. MUC5AC expression was significantly higher in pancreatic adenocarcinoma than in healthy controls and chronic pancreatitis patients. PMID: 27845339
  22. Cigarette smoke exposure initially triggers the production of mitoROS and subsequently elicits autophagy in airway epithelial cells, which then regulate MUC5AC expression partially through modulation of the JNK signaling and the transcription factor AP-1. PMID: 27084849
  23. this study shows that S-allylmercapto-l-cysteine can suppress MUC5AC secretion using the cell model of chronic obstructive pulmonary disease PMID: 27517516
  24. This paper aims at developing a quantitative computer-aided methodology for automated identification of mucin areas and its percentage using tissue histological images PMID: 26971129
  25. MUC5B was significantly more often detected in middle ear effusion fluid relative to MUC5AC. MUC5AC presence was not significantly different in mucoid and serous fluid. PMID: 27729120
  26. outcome, while the gain of aberrant expression of MUC5AC and particularly of MUC6 was associated with favorable outcome in colorectal cancer, notably in intermediate stages II and III PMID: 27298226
  27. Low mucin1 expression is associated with peritoneal dissemination of gastrointestinal cancer. PMID: 27193208
  28. The HNE-TACE signalling pathway has an important role in the process of MUC5AC overexpression in chronic rhinosinusitis. PMID: 26881964
  29. Knockdown of MUC5AC in SW620 cells remarkably suppressed cell vitality and promoted apoptosis and G1 cell cycle arrest, resulting in the impaired ability of colony formation. The inhibition of MUC5AC in SW620 cells dramatically repressed the cell migration and invasion. PMID: 27610469
  30. These results suggest that IFN-gamma represses MUC5AC expression, disturbing binding of Sp1 to its target sequences. PMID: 27324793
  31. this study shows that SYK increased MUC5AC expression via ERK2 and p38 MAPK signaling pathways in airway epithelial cells PMID: 26980390
  32. The interference of p38 gene expression inhibited the release of IL-1beta and TNF-alpha in cultured cells. It also depressed both mRNA and protein levels of MUC5A. PMID: 26722604
  33. The expression of messenger RNAs is inhibited by EGFR tyrosine kinase inhibitor PMID: 26867523
  34. is a major component of the mucus produced by airway epithelial cells. PMID: 26100173
  35. Review-Meta-analysis: decreased Muc5AC expression might be a poor prognostic predictor for gastric cancer. PMID: 26420972
  36. Data show that verproside inhibits tumor necrosis factor alpha-induced mucin 5AC (MUC5AC) expression through NF-kappa B pathway. PMID: 26318254
  37. Overexpression of MUC5AC has been described in idiopathic pulmonary fibrosis lungs. Read More: http://www.atsjournals.org/doi/full/10.1164/rccm.201507-1322LE#.V2WAGNLrtNs PMID: 26871672
  38. The expression of ALPi and MUC5AC in cocultures of Caco-2 and HT29 cells developed for permeability studies is reported. PMID: 26299896
  39. MUC5AC hypomethylation is predictive of serrated lesions with malignant potential PMID: 26476272
  40. EGF removal or tyrphostin AG1478 treatment of differentiating air-liquid interface cultures from asthmatic children would result in a reduction of epithelial goblet cells and mucus secretion. PMID: 26057128
  41. These data lend support to the notion that beta2AR-agonists may contribute to asthma exacerbations by increasing mucin production via activation of beta2ARs on epithelial cells. PMID: 26161982
  42. The measurement of the CA 19-9 antigen on individual proteins could improve performance over the standard CA 19-9 assay. PMID: 22220206
  43. Studied the effects of 15-HETE on MUC5AC expression and related pathways. PMID: 25649981
  44. Increased expression of MUC5AC was associated with bacterial biofilm formation in chronic rhinosinusitis patients. PMID: 25638393
  45. Leukotriene E4 induces MUC5AC release from human airway epithelial NCI-H292 cells PMID: 25838093
  46. Results showed that MUC5AC expression is correlated to the tumor stage where advanced gastric cancers present reduced levels of MUC5AC and that Gli regulates its expression via interaction with a highly conserved sequence in its promoter region. PMID: 25166306
  47. MUC5AC expression in ovarian mucinous tumors did not change from benign to malignant tumors. PMID: 25298197
  48. Common genetic variations in the MUC5AC gene are not related to helicobacter pylori serologic status. PMID: 25605164
  49. The genetic length variants in the central repetitive region of MUC5AC were not associated with meconium ileus in cystic fibrosis. PMID: 24920497
  50. Data suggest that determination of mucin 5AC (MUC5AC) methylation status may be useful for understanding and predicting the natural history of colorectal cancers (CRC). PMID: 25403854

Show More

Hide All

Subcellular Location Secreted
Tissue Specificity Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.
Database Links

HGNC: 7515

OMIM: 158373

KEGG: hsa:4586

STRING: 9606.ENSP00000435591

UniGene: Hs.534332

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1