| Description |
The product CSB-E10109h is a ready-to-use ELISA kit for the quantification of mucin-5AC (MUC5AC) in human serum, plasma, cell culture supernates, or urine. This assay employs the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction to achieve the measurement. The solution develops in proportion to the amount of MUC5AC in the sample. And the color intensity can be measured at 450 nm through a microplate reader. The kit has undergone rigorous quality validation in many aspects, including sensitivity, specificity, precision, recovery, linearity, and consistency between batches.
MUC5AC is a gel-forming mucin with multiple domains containing a heavily O-glycosylated apoprotein core and extensive intramolecular disulfide bonds between the cysteine-rich amino and carboxy-terminal domains. It exerts versatile effects in many biological processes, including barrier functions to epithelial cells, interactions between host and pathogen, immune cell attraction to sites of premalignant or malignant lesions, and tumor progression in a context-dependent manner. Differential expression, glycosylation, and localization of MUC5AC have been linked to a variety of benign and malignant pathologies. |
| Target Name |
mucin-5AC |
| Alternative Names |
MUC5AC ELISA Kit; MUC5 ELISA Kit; Mucin-5AC ELISA Kit; MUC-5AC ELISA Kit; Gastric mucin ELISA Kit; Major airway glycoprotein ELISA Kit; Mucin-5 subtype AC ELISA Kit; tracheobronchial ELISA Kit; Tracheobronchial mucin ELISA Kit; TBM ELISA Kit |
| Abbreviation |
MUC5AC |
| Uniprot No. |
P98088 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, urine |
| Detection Range |
15.6 ng/mL-1000 ng/mL |
| Sensitivity |
3.9 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction
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| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
87-99 |
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| 1:2 |
Average % |
94 |
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| Range % |
87-96 |
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| 1:4 |
Average % |
98 |
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| Range % |
94-106 |
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| 1:8 |
Average % |
90 |
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| Range % |
86-97 |
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| Recovery |
| The recovery of human MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
87 |
95-93 |
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| EDTA plasma (n=4) |
94 |
91-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.452 |
2.563 |
2.508 |
2.375 |
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| 500 |
1.949 |
1.964 |
1.957 |
1.824 |
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| 250 |
1.358 |
1.431 |
1.395 |
1.262 |
|
| 125 |
0.812 |
0.832 |
0.822 |
0.689 |
|
| 62.5 |
0.536 |
0.576 |
0.556 |
0.423 |
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| 31.2 |
0.342 |
0.356 |
0.349 |
0.216 |
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| 15.6 |
0.241 |
0.249 |
0.245 |
0.112 |
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| 0 |
0.132 |
0.134 |
0.133 |
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| Materials provided |
- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human MUC5AC antibody.This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labled MUC5AC antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
