Human thioredoxin reductase,TrxR ELISA kit

Code CSB-E09731h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q16881
Lead Time 3-5 working days
Protein Biological Process 2 Anion transport
Abbreviation TXNRD1
Protein Biological Process 1 Transport
Target Name thioredoxin reductase 1
Alias GRIM-12, MGC9145, TR, TR1, TRXR1, TXNR, KM-102-derived reductase-like factor|oxidoreductase|thioredoxin reductase GRIM-12
Species Homo sapiens (Human)
Protein Biological Process 3 Electron transport
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3 UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.
HGNC 12437
RGD 61959
MGI 1354175
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TrxR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 99
Range % 87-104
1:2 Average % 101
Range % 94-105
1:4 Average % 94
Range % 85-99
1:8 Average % 100
Range % 84-105
Recovery
The recovery of human TrxR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 83-94
EDTA plasma (n=4) 102 91-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 1.994 1.943 1.969 1.787
10 1.555 1.501 1.528 1.346
5 1.091 1.062 1.077 0.895
2.5 0.776 0.784 0.780 0.598
1.25 0.484 0.476 0.480 0.298
0.625 0.359 0.347 0.353 0.171
0.312 0.270 0.263 0.267 0.085
0 0.185 0.179 0.182  
References
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Function Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
Subcellular Location Cytoplasm, SUBCELLULAR LOCATION: Isoform 4: Cytoplasm, Nucleus, SUBCELLULAR LOCATION: Isoform 5: Cytoplasm
Protein Families Class-I pyridine nucleotide-disulfide oxidoreductase family
Tissue Specificity Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus,
Database Links

HGNC: 12437

OMIM: 601112

KEGG: hsa:7296

STRING: 9606.ENSP00000434516

UniGene: Hs.654922

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