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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of mouse ADAM8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||105||95-110|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
The ELISA Kit is designed for quantitatively measuring mouse ADAM8 levels in samples, including serum, plasma, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ADAM8 in samples. The color intensity is measured at 450 nm via a microplate reader.
ADAM8 is a proteolytically active member of the ADAM protease family, which contains disintegrin and metalloprotease domains. The dysregulation of ADAM8 influences pathological conditions such as rheumatoid arthritis, asthma, and cancers. Abnormal expression of ADAM8 has been found in solid tumors, such as gliomas, lung cancer, pancreatic cancer, renal cell carcinomas, and prostate carcinomas. ADAM8 overexpression has been related to poor prognosis in hepatocellular carcinoma, breast cancer, and pancreatic adenocarcinoma, and might act as a potential therapeutic target. ADAM8 is implicated in tumorigenesis by stimulating angiogenesis, increasing cellular abilities of invasion and migration, and inhibiting cancer cell apoptosis.
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