Mouse A Disintegrin And Metalloprotease 8,ADAM8 ELISA Kit

Code CSB-E08817m
Size 96T,5×96T,10×96T
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Product Details

Target Name
ADAM metallopeptidase domain 8
Alternative Names
Adam8 ELISA kit; Ms2Disintegrin and metalloproteinase domain-containing protein 8 ELISA kit; ADAM 8 ELISA kit; EC 3.4.24.- ELISA kit; Cell surface antigen MS2 ELISA kit; Macrophage cysteine-rich glycoprotein ELISA kit; CD antigen CD156a ELISA kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
15.6 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 86-98
1:2 Average % 97
Range % 92-107
1:4 Average % 93
Range % 87-98
1:8 Average % 87
Range % 82-94
The recovery of mouse ADAM8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 87-97
EDTA plasma (n=4) 105 95-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 2.226 2.344 2.285 2.171
2000 1.806 1.923 1.865 1.751
1000 1.341 1.354 1.348 1.234
500 0.723 0.750 0.737 0.623
250 0.440 0.411 0.426 0.312
125 0.278 0.287 0.283 0.169
62.5 0.213 0.205 0.209 0.095
0 0.112 0.115 0.114  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

The ELISA Kit is designed for quantitatively measuring mouse ADAM8 levels in samples, including serum, plasma, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ADAM8 in samples. The color intensity is measured at 450 nm via a microplate reader.

ADAM8 is a proteolytically active member of the ADAM protease family, which contains disintegrin and metalloprotease domains. The dysregulation of ADAM8 influences pathological conditions such as rheumatoid arthritis, asthma, and cancers. Abnormal expression of ADAM8 has been found in solid tumors, such as gliomas, lung cancer, pancreatic cancer, renal cell carcinomas, and prostate carcinomas. ADAM8 overexpression has been related to poor prognosis in hepatocellular carcinoma, breast cancer, and pancreatic adenocarcinoma, and might act as a potential therapeutic target. ADAM8 is implicated in tumorigenesis by stimulating angiogenesis, increasing cellular abilities of invasion and migration, and inhibiting cancer cell apoptosis.

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Target Background

(From Uniprot)
Possible involvement in extravasation of leukocytes.
Gene References into Functions
  1. ADAM8 on leukocytes holds a proinflammatory function in acute lung inflammation by promoting alveolar leukocyte recruitment. PMID: 28596294
  2. The important role of ADAM8 in the progression of hepatocellular carcinoma induced by diethylnitrosamine in mice PMID: 25585999
  3. ADAM8 mediates an enhanced invasiveness of neutrophils into injured muscle fibers by the removal of their adhesiveness to blood vessels after infiltration into interstitial tissues. PMID: 25511460
  4. ADAM8 has anti-inflammatory activities during allergic airway inflammation in mice PMID: 23670189
  5. overexpression of PrP(C) led to up-regulation of ADAM8, suggesting that PrP(C) may regulate its own alpha-cleavage through modulating ADAM8 activity. PMID: 22447932
  6. ADAM-8 appears to favour allergen-induced acute airway inflammation by promoting dendritic cell recruitment and CCL11 and CCL22 production. PMID: 21268008
  7. ADAM8 knockout mice did not display a bone phenotype in vivo but they did not increase RANKL production, OCL formation, or calvarial fibrosis in response to tumor necrosis factor alpha (TNF-alpha) in vivo. PMID: 20683884
  8. ADAM8 is involved in T cell maturation in the medulla but has a relatively minor impact on T cell development. PMID: 20856819
  9. It regulates onset of blood cirlation. (review) PMID: 21077325
  10. The results of this study indicated an essential role for ADAM8 in modulating TNF-alpha signaling in CNS diseases PMID: 20826683
  11. The study finds an increase in retinal re-vascularization but fewer neovascular tufts in the oxygen-induced retinopathy model and increased growth of heterotopically injected tumor cells in Adam8-/- mice compared with wild-type controls. PMID: 20119708
  12. ADAM8 plays a proinflammatory role in airway inflammation. PMID: 20194813
  13. ADAM8 processing by autocatalysis leads to a potential sheddase and to a form of ADAM8 with a function in cell adhesion PMID: 12372841
  14. ADAM8 could contribute to ectodomain shedding of CD23 and may thus be a potential target for therapeutic intervention in allergy and inflammation. PMID: 12777399
  15. Adam8 was identified as the command gene of the group in WT (WildType) and Mmp2 in KO (connexin43-null) mice. PMID: 14756057
  16. ADAM8 plays an important role in physiological and pathological cell interactions by a specific release of functional cell adhesion molecule CHL1 from the cell surface PMID: 14761956
  17. ADAM8 is an allergen-, IL-4-, and IL-13-induced gene in the experimental asthmatic lung. Taken together with the role of ADAM33 in asthma, these results suggest that allergic lung responses involve the interplay of diverse members of the ADAM family PMID: 15087305
  18. developmental expression analysis of ADAM8 in mice PMID: 15580619
  19. observations suggest that a disintegrin and metalloproteinase (ADAM)-8,-9,-10,-12,-15,and -17 play an important role in mouse uterine tissue remodelling during the oestrous cycle PMID: 15907280
  20. ADAM8-mediated shedding of vascular cell adhesion molecule might be important for the suppression of experimental asthma PMID: 16154205
  21. These results suggest that regulated metalloprotease-induced ectodomain shedding of NCAM down-regulates neurite branching and neurite outgrowth. PMID: 16967505
  22. ADAM8 is expressed in a stage-specific manner and is hormonally regulated in ovulating follicles by the coordinate actions of LH and PGR PMID: 18287572
  23. These data suggest that ADAM8 is vascular selective and plays a role in proliferation and/or migration of endothelial cells during angiogenesis following spinal cord injury. PMID: 19003792
  24. Upregulation of ADAM8 in the airways of mice with allergic bronchial asthma. PMID: 19373511

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Database Links
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