Mouse Gelsolin ELISA Kit

Instructions
Code CSB-E08162m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name gelsolin (amyloidosis, Finnish type)
Alternative Names Gsn ELISA kit; GsbGelsolin ELISA kit; Actin-depolymerizing factor ELISA kit; ADF ELISA kit; Brevin ELISA kit
Abbreviation GSN
Uniprot No. P13020
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Gelsolin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 102    
Range % 98-107    
1:200 Average % 96    
Range % 92-100    
1:400 Average % 91    
Range % 86-95    
1:800 Average % 93    
Range % 89-97    
Recovery
The recovery of mouse Gelsolin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 85-93    
EDTA plasma (n=4) 84 80-88    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
1000 2.741 2.578 2.660 2.556    
500 1.903 1.827 1.865 1.761    
250 1.195 1.210 1.203 1.099    
125 0.650 0.685 0.668 0.564    
62.5 0.378 0.362 0.370 0.266    
31.2 0.262 0.249 0.256 0.152    
15.6 0.184 0.191 0.188 0.084    
0 0.101 0.106 0.104      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Gene References into Functions
  1. Induction of cell stress alters the actin cytoskeleton in intestinal epithelial cells via changes in the actin-binding proteins villin-1 and gelsolin. PMID: 29274870
  2. Myelin loss and subsequent axon degeneration contributes to a variety of neurological diseases, such as multiple sclerosis (MS). PMID: 28193690
  3. free actin likely contributes to impaired host defense by blocking scavenger receptor binding of bacteria. PMID: 28385809
  4. gelsolin plays a critical role in airway smooth muscle relaxation. PMID: 28118027
  5. We conclude that a nanobody-based gene therapy using adeno-associated viruses shows great potential as a novel strategy in gelsolin amyloidosis and potentially other amyloid diseases. PMID: 28334940
  6. The data presented here reveal an additional level of interplay between CCT and actin mediated via gelsolin, suggesting that CCT may influence processes depending on gelsolin activity, such as cell motility. PMID: 26364302
  7. novel roles for actin-depolymerizing factor and cofilin-1 in regulating the remodeling and permeability of epithelial junctions PMID: 26878213
  8. The data of this study identify overlapping functions for ADF and n-cofilin in presynaptic physiology and vesicle in mice. trafficking. PMID: 24770705
  9. GSNs might play a regulatory role in the suppression of the tissue damage induced by acute radiation exposure PMID: 25164111
  10. Influence of gelsolin deficiency on excitation contraction coupling in adult murine cardiomyocytes PMID: 26084219
  11. our data demonstrate that a cell-autonomous program consisting of a stress-induced Atf3-gelsolin cascade affects the change in dendritic spine morphology in models of tuberous sclerosis complex PMID: 26224859
  12. Plasma gelsolin levels decrease in the blood of mice with type II diabetes. PMID: 25478578
  13. The actin-binding proteins eps8 and gelsolin have complementary roles in regulating the growth and stability of mechanosensory hair bundles of mammalian cochlear outer hair cells. PMID: 24475274
  14. Burn-induced cerebral gelsolin expression is involved in the activation of both monocytes and astroglial cells, thereby playing a crucial role in the subsequent inflammation-induced neural apoptosis following burn injury. PMID: 23079629
  15. study concludes that cell adhesion to collagen presented on beads activates Ca(2 ) entry and promotes the formation of phagosomes enriched with NMMIIA and gelsolin PMID: 23325791
  16. The F-actin severing activity of ADF/Co fi lin organizes space for the protrusion and bundling of microtubules, the backbone of neurites. ADF/Co fi lin organizes the cytoskeleton to drive actin retrograde fl ow and thus break the spherical shape of neurons. PMID: 23259946
  17. Gelsolin deficiency alters the equilibrium between erythrocyte actin polymerization and depolymerization, causing impaired terminal maturation. PMID: 22271892
  18. data support a mechanistic model for gelsolin's role in insulin exocytosis: gelsolin clamps unsolicited soluble N-ethylmaleimide-sensitive factor attachment receptor-regulated exocytosis through association with Syn4 which is relieved upon stimulus-induced calcium influx to activate gelsolin to facilitate insulin exocytosis PMID: 22108804
  19. The ablation of actin-depolymerizing factor did not change cell death progression. PMID: 22139132
  20. Gsn knockdown suppressed expression of lipogenic genes but strikingly increased that of Tnfalpha and Il6, which may have connections with lipolytic capacity of these genes. PMID: 22445754
  21. gelsolin and NM myosin IIA interact at collagen adhesion sites to enable NM myosin IIA filament assembly and localized, Ca(2+)-dependent remodeling of actin at the nascent phagosome PMID: 21828045
  22. Data show that dendritic cells form podosomes independently of gelsolin, that there are no major alterations in their dynamics of formation and disassembly, and that they exhibit matrix-degrading function. PMID: 21779330
  23. identify the actin filament remodeling protein gelsolin as a CCT-binding partner, and although it does not behave as a classical folding substrate, gelsolin binds to the molecular chaperone CCT with a degree of specificity PMID: 20890741
  24. Gelsolin was identified as the additional interacting partners to p55 in the whirlin interactome of hair cell stereocilia. PMID: 20661277
  25. Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics PMID: 20395300
  26. Gelsolin is necessary for macrophage motility in culture, and its absence is likely to impair the recruitment of macrophages to the injury site. PMID: 20014276
  27. Increased hippocampal neurogenesis in Gsn(-/-) mice was associated with a special microenvironment characterized by enhanced density of perfused vessels, increased regional cerebral blood flow, and increased NOS-III. PMID: 20203201
  28. explores the structure-function relationship of the gelsolin-PPI interaction PMID: 12270712
  29. Results suggest that proline-rich tyrosine kinase 2 (PYK2) is a regulator of gelsolin, revealing a novel PYK2-gelsolin pathway in regulating actin cytoskeletal organization in multiple cells, including osteoclasts. PMID: 12578912
  30. Deficiency of gelsolin in mice affects pulmonary vascular permeability and ischemic injury. PMID: 12654637
  31. Ability of gelsolin to remodel actin filaments is important for collagen-induced calcium entry PMID: 14617805
  32. Actin remodeling by endogenous gelsolin or analogues protects neurons from apoptosis mediated by mitochondria and caspase-3. PMID: 14962741
  33. concluded that gelsolin is the primary Ca2+-sensitive actin filament recycling protein in the cell and is capable of enhancing Listeria actin tail disassembly at normal resting [Ca2+]i PMID: 15671163
  34. separate functions of gelsolin mediate sequential stages of collagen phagocytosis: Ca2+-dependent actin severing facilitates collagen binding, whereas PI(4,5)P2-dependent regulation of gelsolin promotes the actin assembly PMID: 16120646
  35. Results suggest that the spatiotemporal upregulation of gelsolin in the hippocampus is induced by entorhinal deafferentation. PMID: 16261560
  36. Gelsolin exerts an overall anti-apoptotic effect in vivo. PMID: 16507893
  37. Gelsolin plays a pro-survival and apoptotic role in pancreatic beta-cells. PMID: 17192468
  38. PIP5KIgamma-mediated generation of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) at sites of N-cadherin contacts regulates intercellular adhesion strength, an effect due in part to PI(4,5)P2-mediated regulation of gelsolin. PMID: 17538019
  39. GSN was up-regulated in the airways upon ventilator-induced lung injury. Genetic ablation led to protection. GSN fragmentation was an effector mechanism in VILI-induced apoptosis. GSN expression was necessary for efficient neutrophil infiltration PMID: 19202007
  40. Gelsolin regulates cardiac remodeling after myocardial infarction through DNase I-mediated apoptosis. PMID: 19246681

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Subcellular Location Isoform 2: Cytoplasm, cytoskeleton, SUBCELLULAR LOCATION: Isoform 1: Secreted
Protein Families Villin/gelsolin family
Database Links

KEGG: mmu:227753

STRING: 10090.ENSMUSP00000028239

UniGene: Mm.21109

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