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| Code | CSB-E08809m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 92-99 | |||||
| The recovery of mouse Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 94 | 88-99 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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CUSABIO's Kim-1 mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Kim-1 in serum, plasma, tissue homogenates, or urine. This assay uses an antibody specific for mouse Kim-1 coated on a 96-well plate. Kim-1 present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated Kim-1 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of Kim-1 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.
Kim-1 (HAVCR1) is most highly upregulated in the proximal tubular epithelium in acute and chronic kidney injury. Kim-1 functions as a phosphatidylserine receptor that recognizes and internalizes apoptotic cells and also acts as a scavenger receptor that mediates the uptake of modified low-density lipoprotein and necrotic-cell debris. A large amount of Kim-1 protein is also released into the urine, making it a useful urinary biomarker for kidney injury. In the immune system, Kim-1 is also involved in activating the differentiation of Th1, Th2, and Th17. And Kim-1 is also considered as an activating receptor in B cells, dendritic cells, and natural killer T cells.
Sample type: Plasma (anticoagulant)
Sample species: Mouse
Sample dilution: 1:3
Review: The sample was heparin anticoagulant plasma, diluted 1:3 for testing, and the result was OK.
By Anonymous
