Mouse Nephrin(NPHS1) ELISA kit

Code CSB-EL015988MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
nephrosis 1, congenital, Finnish type (nephrin)
Alternative Names
Nphs1 ELISA Kit; Nphn ELISA Kit; Nephrin ELISA Kit; Renal glomerulus-specific cell adhesion receptor ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
250 pg/mL-4000 pg/mL
250 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
To assess the linearity of the assay, samples were spiked with high concentrations of mouse NPHS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 92    
Range % 86-98    
1:200 Average % 97    
Range % 92-102    
1:400 Average % 85    
Range % 82-88    
1:800 Average % 88    
Range % 83-94    
The recovery of mouse NPHS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
Sample Type Average % Recovery Range    
Serum (n=5) 92 87-96    
EDTA plasma (n=4) 94 90-97    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
pg/ml OD1 OD2 Average      
4000 0.110 0.115 0.113      
2000 0.287 0.298 0.293      
1000 0.535 0.544 0.540      
500 0.862 0.848 0.855      
250 1.366 1.375 1.371      
0 2.502 2.523 2.513      
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse NPHS1 ELISA Kit was designed for the quantitative measurement of Mouse NPHS1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 250 pg/mL-4000 pg/mL and the sensitivity is 250 pg/mL.

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Target Background

(From Uniprot)
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Gene References into Functions
  1. Nephrin is necessary for podocyte recovery following injury in an adult mature glomerulus PMID: 29924795
  2. Here, we found the dynamic changes of Notch1 were coincided with autophagy: they both increased during kidney development and podocyte better understand the relationship between Notch signalling and autophagy in podocyte differentiation, rapamycin was added to enhance autophagy levels in DAPT-treated cells, and as a result, nephrin was recovered and DAPT-induced injury was ameliorated PMID: 28158917
  3. WHSC1L1-L acts as a histone methyltransferase in podocytes and regulates nephrin gene expression, which may in turn contribute to the integrity of the slit diaphragm of the glomerular filtration barrier. PMID: 28228401
  4. Data (including data from studies using transgenic mice, an murine experimental model of diabetes, and mouse/human cell lines) suggest prostaglandin I2 receptor (Ptgir) is involved in insulin secretion in pancreatic beta-cells and in permselectivity in glomerular podocytes; the mechanism appears to involve regulation of post-translational phosphorylation of nephrin. PMID: 26868296
  5. the interaction between nephrin and MAGI-1 regulates Rap1 activation in podocytes to maintain long term slit diaphragm structure PMID: 27707879
  6. interaction of full-length suPAR with alphavbeta3 integrin expressed on podocytes results in down-modulation of nephrin that may affect kidney functionality in different human pathologies characterized by increased concentration of suPAR. PMID: 26380915
  7. Nphs1 is an activity dependent gene in mouse olfactory sensory neurons. PMID: 24692514
  8. Nephrin is involved in pancreatic beta-cell survival signaling; a marked decrease in nephrin expression and phosphorylated Akt was observed in pancreatic islets of leptin receptor-deficient diabetic mice. PMID: 25448064
  9. SHP-1 contributes to nephrin deactivation in podocytes exposed to high glucose levels. PMID: 25404734
  10. The aPKC-Par3 complex regulates the cell-surface localization of nephrin. PMID: 24700503
  11. The planar cell polarity pathway contributes to podocyte development by regulating nephrin turnover during junctional remodeling as the cells differentiate. PMID: 23824190
  12. Robo2 signaling acts as a negative regulator on nephrin to influence podocyte foot process architecture. PMID: 22840396
  13. Notch signaling induces endocytosis of nephrin, thereby triggering the onset of proteinuria. PMID: 22052054
  14. 1,25(OH)(2)D(3) stimulates nephrin expression in podocytes by acting on a VDRE in the proximal nephrin promoter. PMID: 21803771
  15. Data show that the main slit diaphragm proteins, nephrin and podocin, are affected from the earlier stages of lupus nephritis and their expression correlates with disease histology. PMID: 21478284
  16. Nephrin directly interacts with p75NTR and reveals an important role for nephrin in murine cardiac development by permitting survival of cardiovascular progenitor cells. PMID: 21402589
  17. PKC alpha mediates beta-arrestin2-dependent nephrin endocytosis in hyperglycemia. PMID: 21321125
  18. PKCalpha is involved in reduction of nephrin surface expression; activation of PKCalpha is a pathomechanistic key event during the development of diabetic nephropathy PMID: 20419132
  19. HGF protects podocytes from a loss of nephrin, at least in part, through maintaining synaptopodin in nephritic mice with albuminuria. PMID: 21342325
  20. Coexpression of CIN85/Ruk(L) with CD2AP led to a decreased binding of CIN85/Ruk(L) to nephrin and podocin, which indicates a functional competition between CD2AP and CIN85/Ruk(L). PMID: 20457601
  21. Expression of nephrin in podocytes is regulated by the cAMP-RAR pathway and suppression of nephrin by TNF-alpha is caused, at least in part, through selective inhibition of this pathway. PMID: 20237236
  22. These observations indicate that podocyte-specific expression of rat nephrin can rescue nephrin-deficient mice from perinatal death, but is not sufficient for full complementation. PMID: 19948823
  23. Nephrin and Neph3 might regulate certain developmental aspects of Ptf1a-positive neurons as homo- or heterooligomers. PMID: 19887377
  24. nephrin and Neph1 interact and have roles in glomerular permeability PMID: 12865409
  25. Nphs1 contributes to a porous slit diaphragm scaffold, the molecular filter in renal glomerular capillaries. PMID: 15545998
  26. The abnormality of a single podocyte molecule, caused by a single gene mutation in alpha Cd2ap can lead to hereditary nephrotic syndromes. PMID: 16501493
  27. Results elucidated the bidirectional regulation of nephrin by pathophysiologic substances and may provide molecular bases for how proteinuria is induced and why some ligands for nuclear receptors have the anti-proteinuric potential. PMID: 16820792
  28. Our data suggest that PKCalpha activation may be an important intracellular signaling pathway in the regulation of nephrin expression and glomerular albumin permeability in the diabetic state. PMID: 16955103
  29. Expression of nephrin mRNA as well as nephrin promoter activity was suppressed by a putative dedifferentiation factor of podocytes, hepatocyte growth factor. PMID: 17244893
  30. Studies in mice have shown that sFlt-1 may play a role in inducing proteinuria by neutralizing vascular endothelial growth factor (VEGF) and suppressing nephrin and synaptopodin. PMID: 17255128
  31. Nuclear relocation of nephrin promotes apoptosis of podocytes. PMID: 17537921
  32. Nephrin-Neph1 complex transduces phosphorylation-mediated signals that assemble an actin polymerization complex at the podocyte intercellular junction. PMID: 17923684
  33. Nephrin does not seem to have a primary role in cell survival but has a small impact on gene regulation during glomerular development. PMID: 18046313
  34. Neph1-Nephrin proteins bind the Par3-Par6-atypical protein kinase C (aPKC) complex to regulate podocyte cell polarity PMID: 18562307
  35. Results suggest that Kirrel3 plays a role in the axonal pathfinding, cell recognition, and synapse formation of DRG neurons on target cells, including the targeting of proprioceptive neurons on muscle spindles through the interaction with nephrin. PMID: 18752272
  36. Results suggest that tensin2 is involved in expression and extension of nephrin, while tensin2 deficiency may result in proteinuria, associated with the loss of slit integrity. PMID: 19129671
  37. The aPKC-PAR3 complex associates with the nephrin-podocin complex in podocytes through direct interaction between PAR3 and nephrin, and the kinase activity of aPKC is required for the appropriate distribution of nephrin and podocin in podocytes. PMID: 19142224
  38. Nephrin Tyr-1204 phosphorylation triggers the Ca(2+) response in a PLC-gamma1-dependent fashion. PMID: 19179337
  39. Nck is required to maintain adult podocytes and phosphotyrosine-based interactions with nephrin may occur in foot processes of resting, mature podocytes. PMID: 19443634
  40. These studies suggest that nephrin plays a role in secondary fusion of myoblasts into nascent myotubes, thus establishing a possible functional conservation with Drosophila Sns. PMID: 19470472
  41. results suggested selective cooperation of RAR and VDR in the regulation of the nephrin gene PMID: 19474283
  42. Nephrin may normally limit NF-kappaB activity in the podocyte. PMID: 19497968
  43. In experimental diabetes, MCP-1 was overexpressed within the glomeruli and the absence of MCP-1 reduced both albuminuria and downregulation of nephrin and synaptopodin PMID: 19587356

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Immunoglobulin superfamily
Tissue Specificity
Expressed in kidney glomeruli. In the embryo, expressed in the mesonephric kidney at E11 with strong expression in cranial tubules with podocyte-like structures. Expression is observed in the podocytes of the developing kidney from E13. High expression is
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