Mouse Pulmonary Surfactant-associated protein B (SP-B) ELISA kit

Code CSB-E12638m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
surfactant protein B
Alternative Names
Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.45 ng/mL-30 ng/mL
0.11 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %86-97
1:2Average %97
Range %92-102
1:4Average %88
Range %85-93
1:8Average %89
Range %83-94
The recovery of mouse SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9187-96
EDTA plasma (n=4)10296-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
302.176 2.206 2.191 2.031
151.511 1.544 1.528 1.368
7.50.897 0.970 0.934 0.774
3.750.612 0.618 0.615 0.455
1.80.362 0.392 0.377 0.217
0.90.279 0.267 0.273 0.113
0.450.225 0.220 0.223 0.063
00.156 0.163 0.160
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse SFTPB ELISA Kit was designed for the quantitative measurement of Mouse SFTPB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL.


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Target Background

(From Uniprot)
Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Gene References into Functions
  1. Surfactant protein B suppresses lung cancer progression by inhibiting secretory phospholipase A2 activity and arachidonic acid production. PMID: 28743125
  2. These data provided a plausible mechanism linking maternal vitamin D deficiency with altered postnatal lung function. PMID: 27121020
  3. Surfactant protein B had decreased levels in influenza A virus PR8-infected cells, which was confirmed by immunoblotting and immunofluorescence assays. PMID: 25965799
  4. macrophages participate in the repression of SFTPB expression by LPS, and that macrophage-released cytokines (including TNF) regulate the transcription factor CEBPB PMID: 23590297
  5. ErbB4 regulates the expression of surfactant protein B in fetal lung maturation, helping to prepare for the fetal-neonatal transition. PMID: 21317380
  6. The stimulatory effects of estrogen on Sftpb are under transcriptional control of ErbB4. PMID: 21777626
  7. SP-B contributes to innate host defense of the lung by supplementing the nonoxidant antimicrobial defenses of alveolar macrophages PMID: 20007532
  8. DNA methylation and chromatin modifications cooperate with Nkx2-1 to regulate Sftpb gene cell specific expression. PMID: 19906647
  9. formation of the intersubunit disulfide bridge is not critical for SP-B function PMID: 12133857
  10. The late asthmatic response is linked with increased surface tension and reduced surfactant protein B in mice. PMID: 12225952
  11. This protein inhibits endotoxin-induced lung inflammation. PMID: 12594064
  12. Reduction of alveolar SP-B content causes surfactant dysfunction and respiratory failure, indicating that SP-B is required for postnatal lung function. PMID: 12639841
  13. SP-B expression is regulated by PPARgamma in the lung PMID: 12829715
  14. napsin, cathepsin H, and at least one other enzyme are involved in maturation of the biologically active SP-B peptide PMID: 14766755
  15. Results indicate that hypoxia-induced mitogenic factor induces surfactant protein B and C production in mouse lungs and cell lines via activation of phosphatidylinositol 3-kinase/Akt and ERK1/2 mitogen-activated protein kinases. PMID: 16166744
  16. absence of HO-1 in the lung parenchyma, not in bone marrow-derived inflammatory cells, was responsible for enhanced SP-B downregulation and severe physiologic lung dysfunction PMID: 16309574
  17. Via promoter mutation analysis, adjacent TTF-1 binding sites within the proximal promoter region of SP-C were found to be essential for TTF-1/TAP26-enhanced SP-C promoter activity. PMID: 16630564
  18. SP-B controls surface tension by forming a surfactant phospholipid film that regulates shape and nonspecific phagocytic activity of alveolar macrophages on the alveolar surface. PMID: 16632521
  19. ABCA3 is necessary for lamellar body biogenesis, surfactant protein-B processing, and lung development late in gestation. PMID: 17540762
  20. Three novel Sp1 binding motifs were identified in the mouse SP-B promoter. PMID: 19096034
  21. Results support the idea that maintenance of surfactant-associated protein B expression is critical to survival during acute lung injury. PMID: 19131640
  22. The surface tension-lowering properties of Sftpb play an important role in the regulation of pulmonary vascular perfusion. PMID: 19202005
  23. Maintaining Sftpb is critical to survival during acute lung injury PMID: 19131640

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Subcellular Location
Secreted, extracellular space, surface film.
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