Pig Colipase(CLPS) ELISA kit

Instructions
Code CSB-EL005578PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name colipase, pancreatic
Alternative Names CLPS ELISA Kit; Colipase ELISA Kit; Procolipase II ELISA Kit
Abbreviation CLPS
Uniprot No. P02703
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 mU/mL-1000 mU/mL
Sensitivity 3.9 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig CLPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 82-92
1:2 Average % 99
Range % 94-103
1:4 Average % 103
Range % 98-107
1:8 Average % 95
Range % 90-101
Recovery
The recovery of pig CLPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-105
EDTA plasma (n=4) 104 88-111
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
1000 2.297 2.312 2.305 2.196
500 1.786 1.688 1.737 1.628
250 1.133 1.202 1.168 1.059
125 0.654 0.665 0.660 0.551
62.5 0.389 0.374 0.382 0.273
31.2 0.242 0.254 0.248 0.139
15.6 0.177 0.187 0.182 0.073
0 0.105 0.113 0.109  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase.; FUNCTION
Gene References into Functions
  1. isotropic orientation of colipase which could explain its incapacity to reverse the inhibitory effects of these lipids on the lipase activity PMID: 18052211
  2. Analysis of structures of NMR colipase models and simulations of their interactions with various lipid aggregates, lipid droplet, and bile salt micelle, were carried out to determine and to map the lipid binding sites on colipase. PMID: 18506778
Subcellular Location Secreted
Protein Families Colipase family
Tissue Specificity Expressed by the pancreas.
Database Links

KEGG: ssc:397407

STRING: 9823.ENSSSCP00000001686

UniGene: Ssc.514

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