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| Code | CSB-E13023r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-92 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| The recovery of rat AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This rat AST ELISA Kit is suitable for qualitatively determining AST concentrations in multiple biological fluids, including rat serum, plasma, and tissue homogenates in vitro. AST, also called GOT1, is an important transaminase enzyme in amino acid metabolism that catalyzes the conversion of aspartate and alpha-ketoglutarate to oxaloacetate and glutamate. AST is a part of the malate-aspartate shuttle in the myocardium and participates in gluconeogenesis in the liver and kidney, glyceroneogenesis in the adipose tissue, and the production of neurotransmitters and neuro-glial pathways in the brain. A high AST level in the body can be an indicator of liver disease and other health problems.
This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of rat AST in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-rat AST antibody. Biotin-labeled AST antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the AST in the rat samples. AST binds to the biotinylated anti-rat AST monoclonal antibody. And the biotin on the biotinylated anti-rat AST monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of AST in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.
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Kindly address the below queries:
1. The standard ALT enzyme provided in the kit is from which source ?
2. The Standard ALT enzyme given is active or inactive ?
3. Since we cannot collect more than 150 µL of blood from mice, we can use only 50 µL serum for ELISA. Will it fall within the detectable range ?
