Rat Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit

Code CSB-EL017647RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat PCSK9 ELISA Kit was designed for the quantitative measurement of Rat PCSK9 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 93.75 pg/mL-6000 pg/mL and the sensitivity is 46.88 pg/mL.

Target Name proprotein convertase subtilisin/kexin type 9
Alternative Names
Pcsk9 ELISA Kit; Narc1 ELISA Kit; Proprotein convertase subtilisin/kexin type 9 ELISA Kit; EC 3.4.21.- ELISA Kit; Neural apoptosis-regulated convertase 1 ELISA Kit; NARC-1 ELISA Kit; Proprotein convertase 9 ELISA Kit; PC9 ELISA Kit; Subtilisin/kexin-like protease PC9 ELISA Kit
Abbreviation PCSK9
Uniprot No. P59996
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 46.88 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PCSK9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 92  
Range % 82-98  
1:200 Average % 93  
Range % 87-96  
1:400 Average % 98  
Range % 92-104  
1:800 Average % 87  
Range % 82-95  
Recovery
The recovery of rat PCSK9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-97  
EDTA plasma (n=4) 98 96-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
6000 0.088 0.087 0.088    
3000 0.185 0.188 0.187    
1500 0.358 0.369 0.364    
750 0.624 0.630 0.627    
375 1.130 1.111 1.121    
187.5 1.516 1.500 1.508    
93.75 1.810 1.712 1.761    
0 2.169 2.064 2.117    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Gene References into Functions
  1. up-regulation of intestinal gene expression of LDL-R and PCSK9 following voluntary wheel running PMID: 29938676
  2. This is the first report about the expression of PCSK9 in adult terminal differentiated ventricular cardiomyocytes PMID: 28913715
  3. PCSK9 is regulated in vascular smooth muscle cells by TLR-4 - SAPK/JNK signaling, a pathway important in inflammation and metabolism. PMID: 28232185
  4. Hnf1alpha gene expression is upregulated in the liver of rats with experimentally induced chronic renal failure, showing an association between circulating PCSK9 and TAGs PMID: 26978583
  5. PCSK9 could serve as a novel molecular biomarker for the non-invasive prenatal screening of neural tube defects. PMID: 26691006
  6. coordinated up-regulation of Pcsk9 and Srebf-2 genes expression suggests that SREBF-2 may play a key role in regulation of Pcsk9 gene expression, circulating PCSK9 level, and hypercholesterolemia in experimental CRF PMID: 26481479
  7. LDLR deficiency, hypercholesterolemia and elevated plasma LDL in NS are associated with upregulation of PCSK9 and IDOL. PMID: 24166456
  8. Gene expression of hepatic LDL-R, Lrp1, Pcsk9, SREBP-2 and HMGCoA-r were reduced (p<0.05) in ovariectomy compared to Sham rats. PMID: 24619822
  9. data provided the first line of the evidence that BBR, similar to the Sim, could increase the expression of PCSK9 levels in HFD rats through SREBP-2 activation PMID: 24755036
  10. Elevated serum PCSK9 concentration by ezetimibe, Xuezhikang and pitavastatin alone or in combination was found prior to the alterations of lipid profile in rats. PMID: 24533584
  11. Phaleria macrocarpa fruit aqueous extract reduced body weight/improved blood lipid profile of hypercholesterolemic rats. The improvement is likely to be regulated by LDL receptor and PCSK9. PMID: 21763412
  12. Our functional characterization of Narc 1 reinforces the inference drawn that this enzyme is most closely related to representatives of the proteinase K family, but it is also sufficiently different to warrant its classification in a separate sub-family. PMID: 14622975
  13. Human, mouse, and rat PCSK9 promoters contain 2 typical conserved motifs for cholesterol regulation: a sterol regulatory element (SRE) and an Sp1 site. PMID: 15178557
  14. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is processed in the Endoplasmic Reticulum, and the mature convertase is secreted in the plasma. PMID: 16462892
  15. mechanism by which NARC 1 overexpression induces cell death in cerebellar granule neurons remains unclear; correlation seen between Narc 1 active site serine in transiently expressed protein and induction of BAF-sensitive component of cell death phenotype PMID: 17051583
  16. explaining the lack of reduction in LDL receptor protein levels. These observations indicate that the rate of LDL receptor cycling (function) in diabetic rats is decreased resulting in higher serum LDL levels. PMID: 18054320
  17. The hormonal and dietary regulation of hepatic LDLRs also involves posttranscriptional regulation by PCSK9. PMID: 19008317
  18. NARC-1 is a proteinase K-like subtilase with a role in the differentiation of cortical neurons. PMID: 12552133

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Subcellular Location Cytoplasm. Secreted. Endosome. Lysosome. Cell surface. Endoplasmic reticulum. Golgi apparatus.
Protein Families Peptidase S8 family
Tissue Specificity Highly expressed in 12-day embryo. In the adult, strongly expressed in liver, small intestine, jejunum, and to a lesser extent in kidney, lung, spleen and thymus. Expression in the liver is up-regulated following partial hepatectomy.
Database Links

KEGG: rno:298296

STRING: 10116.ENSRNOP00000008536

UniGene: Rn.19195

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