Recombinant Rat Proprotein convertase subtilisin/kexin type 9(Pcsk9)

Code CSB-YP017647RA
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Source Yeast
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Code CSB-EP017647RA
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Source E.coli
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Code CSB-EP017647RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017647RA
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Source Baculovirus
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Code CSB-MP017647RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Pcsk9
Uniprot No. P59996
Alternative Names Pcsk9; Narc1; Proprotein convertase subtilisin/kexin type 9; EC 3.4.21.-; Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin/kexin-like protease PC9
Species Rattus norvegicus (Rat)
Expression Region 152-691
Target Protein Sequence SIPWNLERI IPAWQQTEED SSPDGSSQVE VYLLDTSIQS GHREIEGRVT ITDFNSVPEE DGTRFHRQAS KCDSHGTHLA GVVSGRDAGV AKGTSLHSLR VLNCQGKGTV SGTLIGLEFI RKSQLIQPSG PLVVLLPLAG GYSRILNTAC QRLARTGVVL VAAAGNFRDD ACLYSPASAP EVITVGATNA QDQPVTLGTL GTNFGRCVDL FAPGKDIIGA SSDCSTCYMS QSGTSQAAAH VAGIVAMMLN RDPALTLAEL RQRLILFSTK DVINMAWFPE DQRVLTPNRV ATLPPSTQET GGQLLCRTVW SAHSGPTRTA TATARCAPEE ELLSCSSFSR SGRRRGDRIE AIGGQQVCKA LNAFGGEGVY AVARCCLLPR VNCSIHNTPA ARAGPQTPVH CHQKDHVLTG CSFHWEVENL RAQQQPLLRS RHQPGQCVGH QEASVHASCC HAPGLECKIK EHGIAGPAEQ VTVACEAGWT LTGCNVLPGA SLPLGAYSVD NVCVARIRDA GRADRTSEEA TVAAAICCRS RPSAKASWVH Q
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Gene References into Functions
  1. up-regulation of intestinal gene expression of LDL-R and PCSK9 following voluntary wheel running PMID: 29938676
  2. This is the first report about the expression of PCSK9 in adult terminal differentiated ventricular cardiomyocytes PMID: 28913715
  3. PCSK9 is regulated in vascular smooth muscle cells by TLR-4 - SAPK/JNK signaling, a pathway important in inflammation and metabolism. PMID: 28232185
  4. Hnf1alpha gene expression is upregulated in the liver of rats with experimentally induced chronic renal failure, showing an association between circulating PCSK9 and TAGs PMID: 26978583
  5. PCSK9 could serve as a novel molecular biomarker for the non-invasive prenatal screening of neural tube defects. PMID: 26691006
  6. coordinated up-regulation of Pcsk9 and Srebf-2 genes expression suggests that SREBF-2 may play a key role in regulation of Pcsk9 gene expression, circulating PCSK9 level, and hypercholesterolemia in experimental CRF PMID: 26481479
  7. LDLR deficiency, hypercholesterolemia and elevated plasma LDL in NS are associated with upregulation of PCSK9 and IDOL. PMID: 24166456
  8. Gene expression of hepatic LDL-R, Lrp1, Pcsk9, SREBP-2 and HMGCoA-r were reduced (p<0.05) in ovariectomy compared to Sham rats. PMID: 24619822
  9. data provided the first line of the evidence that BBR, similar to the Sim, could increase the expression of PCSK9 levels in HFD rats through SREBP-2 activation PMID: 24755036
  10. Elevated serum PCSK9 concentration by ezetimibe, Xuezhikang and pitavastatin alone or in combination was found prior to the alterations of lipid profile in rats. PMID: 24533584
  11. Phaleria macrocarpa fruit aqueous extract reduced body weight/improved blood lipid profile of hypercholesterolemic rats. The improvement is likely to be regulated by LDL receptor and PCSK9. PMID: 21763412
  12. Our functional characterization of Narc 1 reinforces the inference drawn that this enzyme is most closely related to representatives of the proteinase K family, but it is also sufficiently different to warrant its classification in a separate sub-family. PMID: 14622975
  13. Human, mouse, and rat PCSK9 promoters contain 2 typical conserved motifs for cholesterol regulation: a sterol regulatory element (SRE) and an Sp1 site. PMID: 15178557
  14. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is processed in the Endoplasmic Reticulum, and the mature convertase is secreted in the plasma. PMID: 16462892
  15. mechanism by which NARC 1 overexpression induces cell death in cerebellar granule neurons remains unclear; correlation seen between Narc 1 active site serine in transiently expressed protein and induction of BAF-sensitive component of cell death phenotype PMID: 17051583
  16. explaining the lack of reduction in LDL receptor protein levels. These observations indicate that the rate of LDL receptor cycling (function) in diabetic rats is decreased resulting in higher serum LDL levels. PMID: 18054320
  17. The hormonal and dietary regulation of hepatic LDLRs also involves posttranscriptional regulation by PCSK9. PMID: 19008317
  18. NARC-1 is a proteinase K-like subtilase with a role in the differentiation of cortical neurons. PMID: 12552133

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Subcellular Location Cytoplasm. Secreted. Endosome. Lysosome. Cell surface. Endoplasmic reticulum. Golgi apparatus.
Protein Families Peptidase S8 family
Tissue Specificity Highly expressed in 12-day embryo. In the adult, strongly expressed in liver, small intestine, jejunum, and to a lesser extent in kidney, lung, spleen and thymus. Expression in the liver is up-regulated following partial hepatectomy.
Database Links

KEGG: rno:298296

STRING: 10116.ENSRNOP00000008536

UniGene: Rn.19195

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