Rat Vascular endothelial cell growth factor receptor 1,VEGFR-1/Flt1 ELISA Kit

Code CSB-E07350r
Size 96T,5×96T,10×96T
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Product Details

Target Name
fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
Alternative Names
Flt1 ELISA Kit; Flt-1 ELISA Kit; Vegfr1 ELISA Kit; Vascular endothelial growth factor receptor 1 ELISA Kit; VEGFR-1 ELISA Kit; EC ELISA Kit; Fms-like tyrosine kinase 1 ELISA Kit; FLT-1 ELISA Kit; Tyrosine-protein kinase receptor FLT ELISA Kit
Uniprot No.
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 pg/mL-100 pg/mL
0.39 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGFR-1/Flt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 92-101  
1:2 Average % 103  
Range % 99-110  
1:4 Average % 88  
Range % 84-92  
1:8 Average % 95  
Range % 90-100  
The recovery of rat VEGFR-1/Flt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 84 80-88  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.621 2.557 2.589 2.412  
50 2.005 1.946 1.976 1.799  
25 1.286 1.332 1.309 1.132  
12.5 0.893 0.859 0.876 0.699  
6.25 0.514 0.521 0.518 0.341  
3.12 0.397 0.408 0.403 0.226  
1.56 0.260 0.252 0.256 0.079  
0 0.179 0.174 0.177    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

The rat VEGFR-1 ELISA Kit is engineered for accurate measurement of rat VEGFR-1 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the VEGFR-1 content in the sample. The color intensity is positively correlated with VEGFR-1 content in the sample. The VEGFR-1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

VEGFR-1, also called Flt-1, is the exclusive VEGFR present on the surface of monocytes and mediates a chemotactic response to VEGF-A and tissue factor induction. In myeloid cells, VEGFR-1 mediates monocyte migration and has a regulatory role in the development of inflammatory diseases such as rheumatoid arthritis and arteriosclerosis. In vascular smooth muscle cells (SMCs), VEGFR-1 is inducibly expressed by vascular injury and participates in neointimal development. VEGFR-1 expression has frequently been detected in melanoma and is also found in other cancer types. Upon activation by VEGF-A or PlGF, VEGFR-1 promotes cell survival, migration, invasiveness, and chemoresistance of melanoma cells.

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Target Background

(From Uniprot)
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1.
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Endosome.
Protein Families
Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
Database Links
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