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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGFR-1/Flt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of rat VEGFR-1/Flt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||84||80-88|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
The rat VEGFR-1 ELISA Kit is engineered for accurate measurement of rat VEGFR-1 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the VEGFR-1 content in the sample. The color intensity is positively correlated with VEGFR-1 content in the sample. The VEGFR-1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
VEGFR-1, also called Flt-1, is the exclusive VEGFR present on the surface of monocytes and mediates a chemotactic response to VEGF-A and tissue factor induction. In myeloid cells, VEGFR-1 mediates monocyte migration and has a regulatory role in the development of inflammatory diseases such as rheumatoid arthritis and arteriosclerosis. In vascular smooth muscle cells (SMCs), VEGFR-1 is inducibly expressed by vascular injury and participates in neointimal development. VEGFR-1 expression has frequently been detected in melanoma and is also found in other cancer types. Upon activation by VEGF-A or PlGF, VEGFR-1 promotes cell survival, migration, invasiveness, and chemoresistance of melanoma cells.
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