Rat low density lipoprotein receptor,LDLR ELISA Kit

Instructions
Code CSB-E13545r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name low density lipoprotein receptor
Alternative Names Ldlr ELISA Kit; Low-density lipoprotein receptor ELISA Kit; LDL receptor ELISA Kit
Abbreviation LDLR
Uniprot No. P35952
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LDLR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 85
Range % 80-89
1:400 Average % 102
Range % 98-108
1:800 Average % 90
Range % 85-94
1:1600 Average % 97
Range % 92-101
Recovery
The recovery of rat LDLR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 84-92
EDTA plasma (n=4) 93 89-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 2.512 2.475 2.494 2.355
250 2.247 2.196 2.222 2.083
125 1.803 1.689 1.746 1.607
62.5 1.189 1.227 1.208 1.069
31.2 0.687 0.713 0.700 0.561
15.6 0.415 0.392 0.404 0.265
7.8 0.298 0.289 0.294 0.155
0 0.136 0.141 0.139  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.
Gene References into Functions
  1. LDLR is a relevant receptor for CNS drug delivery via receptor-mediated transcytosis and that the peptide vectors we developed have the potential to transport drugs PMID: 28108572
  2. Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol accumulation via increased LDL cholesterol uptake into the liver with resulting persistent increases in hepatic cholesterol accumulation. PMID: 27401460
  3. Compared with the cholesterol-enriched restructured pork group, silicon-enriched restructured pork rats had lower VLDL compound concentrations and lower VLDL oxidation but greater Ldlr expression. PMID: 26246324
  4. The LDL receptor could be located at once in clathrin-coated pits and caveolae. PMID: 24530906
  5. LDLR mutant rats are a useful novel animal model of hypercholesterolemia and atherosclerosis. PMID: 22293196
  6. A tissue-specific dysregulation of LDLr is demonstrated that is age- and gender-dependent. PMID: 21792919
  7. expression of LDLR was increased in the hippocampus of stroke-prone spontaneously hypertensive rats and was seen in the luminal and abluminal cytoplasmic membranes and vesicular structures of endothelial cells PMID: 20458492
  8. expression pattern of LDL-R in brain microvessels endothelial cells and its modulation by astrocytes PMID: 12095612
  9. cloning and sequencing of the rat LDL receptor 5'-flanking region revealed the presence of several putative cis-acting elements PMID: 16647292
  10. Retina is capable of rapid uptake of circulating LDL via LDLR-mediated process primarily occurring in retinal pigment epithelium and also possibly Muller cells. PMID: 17110914
  11. Caloric restriction affects Ldlr involved in age-related hypercholesterolemia. PMID: 17203467
  12. LDLR-mediated pathway was involved in the endocytosis of Poly(methoxypolyethyleneglycol cyanoacrylate-co-hexadecylcyanoacrylate) (PEG-PHDCA) nanoparticles by RBEC. PMID: 17256088
  13. Modeling of corticosteroid effects on hepatic low-density lipoprotein receptors and plasma lipid dynamics in rats. PMID: 17674160

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Membrane, clathrin-coated pit, Golgi apparatus, Early endosome, Late endosome, Lysosome
Protein Families LDLR family
Database Links

KEGG: rno:300438

STRING: 10116.ENSRNOP00000013496

UniGene: Rn.10483

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