Rat thyrotropin-releasing hormone,TRH ELISA KIT

Instructions
Code CSB-E08040r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name thyrotropin-releasing hormone
Alternative Names Trh ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit
Abbreviation TRH
Uniprot No. P01150
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.5 pg/mL-100 pg/mL
Sensitivity 0.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 100  
Range % 96-104  
1:2 Average % 92  
Range % 88-95  
1:4 Average % 93  
Range % 87-96  
1:8 Average % 101  
Range % 97-105  
Recovery
The recovery of rat TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 97 93-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
100 0.150 0.146 0.148    
25 0.301 0.298 0.300    
8 0.487 0.500 0.494    
2 0.776 0.790 0.783    
0.5 1.264 1.288 1.276    
0 1.932 1.907 1.920    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.
Gene References into Functions
  1. Intrauterine Zn deficiency favors thyrotropin-releasing hormone-increasing effects on thyrotropin serum levels and induces subclinical hypothyroidism in weaned rats. PMID: 29057835
  2. We found that dehydration induced anorexia rats showed increased number of thyrotropin-releasing hormone (TRH) cells in caudal paraventricular hypothalamic nucleus, as well as a decreased percentage of TRH-expressing neurons that co-expressed monocarboxylate transporter-8 mRNA signal. PMID: 26626087
  3. The results of this study concluded that long-term changes in dietary Zn down-regulate PPII activity PMID: 26315400
  4. Delayed increases in both the median eminence PPII as well as the thyroliberinase activities in fasted male rats may facilitate the maintenance of deep downregulation of the HPT axis function, despite a partial reactivation of TRH expression in the PVN. PMID: 25942072
  5. TGFbeta2 regulates hypothalamic TRH expression through TIEG1 during fetal development. PMID: 25448845
  6. a specific TRH increase in the left ventricle induced structural changes in the normal heart PMID: 25281568
  7. Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. PMID: 24370184
  8. TRH upregulates the phenotypes of dermal fibroblasts and plays a role in accelerating wound healing. PMID: 24713470
  9. Early-overfed rats presented low proTRH and proCRT synthesis in paraventricular nucleus. PMID: 24464021
  10. Dysfunctional brainstem thyrotropin-releasing hormone (TRH)-elicited vagal impairment contributes to disturbed food intake in type 2 diabetic Goto-Kakizaki rats. PMID: 23701881
  11. NPY (neuropeptide Y) regulates TRH (1) by opposing alpha-MSH (alpha-melanocyte-stimulating hormone) activation of CREB signaling and reducing pro-TRH processing by proprotein convertase 2 and (2) by reducing production/release of alpha-MSH. PMID: 23321476
  12. TRH neurons heavily innervate histaminergic neurons in all subdivisions of the tuberomammillary nucleus. PMID: 23063458
  13. Food-restricted and dehydrated-induced anorexic rats present differential TRH expression in anterior and caudal PVN. Role of type 2 deiodinase and pyroglutamyl aminopeptidase II. PMID: 22719053
  14. E(2) may act as a modulator of the secretory response of lactotrophs induced by TRH through membrane estrogen receptors, with the contribution by PI3K/Akt pathway activation. PMID: 22354782
  15. genes that might participate in the development and function of hypothalamic TRH neurons PMID: 21569245
  16. Klf4 plays a key role in the maturation of TRH expression in hypothalamic neurons. PMID: 21182892
  17. Data suggest that 17b-oestradiol negatively regulates the in vivo expression of thyrotrophin-releasing hormone mRNA in the paraventricular nucleus. PMID: 19302192
  18. These results corroborate the CRE-2 site as the main cAMP-response element of rat TRH promoter. PMID: 21266205
  19. Data suggest a TRH-mediated regulatory mechanism that may underlie the pathologic neuroplasticity driving dopamine hyper-responsivity in Parkinson's disease. PMID: 21085660
  20. These results show for the first time that the cardiac TRH system is involved in the development of left ventricular hypertrophy in SHR. PMID: 21135357
  21. Interaction of cAMP- and glucocorticoid-mediated regulation of TRH transcription at the CRE and cGRE regions of the TRH promoter. PMID: 20136691
  22. results show that pCREB binds to a response element in the TRH promoter (CRE-2) that is independent of Site-4 where thyroid hormone receptor (TR) beta2 is bound; pCREB and TR do not present mutual interference on their binding sites PMID: 19602869
  23. TRH-leptin interaction may contribute to the strong association between hypertension and obesity. PMID: 11882596
  24. Adenohypophyseal pyroglutamyl aminopeptidase II activity and mRNA levels fluctuate during the day and TRH down-regulates this activity in vivo PMID: 12213674
  25. CART expressed in hypophysiotropic TRH neurons may have important role in modulation of TRH-induced prolactin secretion. Increased secretion of CART may cause reduced TRH-induced prolactin response during hypothyroidism. PMID: 14691017
  26. results show that that leptin can regulate trh gene expression via activation of intracellular signal transducer and activator of transcription 3 (STAT3) proteins in TRH neurons in the hypothalamus PMID: 14764629
  27. TRH is specifically induced in the heart after MI and increases cardiac performance in rats with ischemic cardiomyopathy. Pro-TRH/TRH may be another important axis that affects hemodynamics and cardiac function in heart failure. PMID: 15096458
  28. central role of the IB1/JIP-1 protein in the control of TRH-mediated TSH-beta stimulation. PMID: 15345675
  29. Occurrence of VGLUT2 immunoreactivity within TRH and CRH axon varicosities, suggesting terminal glutamate release from these neuroendocrine systems. Hypophysiotropic TRH and CRH neurons possess glutamatergic characteristics. PMID: 15486233
  30. suppression of thyrotropin-releasing hormone gene expression in the hypothalamic paraventricular nucleus by lipopolysaccharide is not caused by brainstem pathways PMID: 15604205
  31. transcription regulated by rapid cross-talk of glucocorticoids with PKA signaling pathways PMID: 15691887
  32. TRH plays an important role in the mechanism of insulin secretion and its response to glucose stimulation. PMID: 15750838
  33. three neuronal phenotypes in the hypothalamic structure that is involved in the induction of attacks: glutamatergic neurons co-expressing thyrotropin releasing hormone, glutamatergic neurons without thyrotropin releasing hormone, and GABAergic neurons PMID: 15908131
  34. Data show that suppression of deiodinase type II (DII) by iopanoic acid during fasting prevented elevated DII activity and blunted the decline in hypothalamic thyrotropin releasing hormone mRNA levels. PMID: 16098513
  35. TRH-like peptides are rapidly modulated in rat brain by thyroid hormones PMID: 16310891
  36. in the hypothalamic paraventricular nucleus there are subpopulations of proTRH neurons responding to leptin, which is dependent upon the way leptin reaches its primary target(s) in the hypothalamus PMID: 16627588
  37. Physiological role of brainstem TRH in vagal-ghrelin-mediated stimulation of food intake, which involves interaction with brainstem Y1 receptors. PMID: 16959836
  38. K8 is a physiological substrate for PKCepsilon, and the phosphorylation at Ser8 and Ser23 transduces, at least in part, TRH-PKCepsilon signaling in pituitary cells PMID: 17553064
  39. Novel information on the molecular mechanisms of control of hypophysiotropic TRH biosynthesis. PMID: 17584968
  40. Results describe the modulation of thyrotropin-releasing hormone (TRH) and TRH-like peptide levels in rat brain and endocrine organs by lipopolysaccharide. PMID: 17726229
  41. These results indicate that hypothalamic neuronal histamine mediates the TRH-induced suppression of feeding behavior. PMID: 17760865
  42. BDNF may contribute to the enhancement of pre-pro-TRH mRNA expression in the hypothalamic paraventricular hypothalamic nucleus during development PMID: 17854778
  43. Response to cold stimulation leading to increased pro-TRH mRNA levels and TRH release was preserved under reduced energy availability in food restrictioned but dehydration-anorexic rats. PMID: 18191132
  44. Data show that protein kinase C and ERK signaling modulate glucocorticoid receptor activity and its interaction with CREB or AP-1 at the TRH gene promoter. PMID: 18427988
  45. Fasting reduced TRH mRNA compared with fed animals in hypothalamic paraventricular nucleus. PMID: 18467436
  46. pro-TRH-derived peptides are differentially sorted within the secretory pathway and that the initial cleavage in the trans-Golgi network is key to this process PMID: 18474603
  47. N-terminal domain within the prohormone sequence does not act as "sorting signal" in late secretion; instead, it seems to play a key role determining the proper folding pathway of the precursor and, thus, its stability PMID: 18779326
  48. TRH acts as a stimulator of vasopressin biosynthesis most of all in young male rats and as an inhibitor for oxytocin biosynthesis especially in mature animals. PMID: 19617647
  49. Released TRH is inactivated by an ectopeptidase, pyroglutamyl aminopeptidase II (PPII). Acute administration of alcohol modulates pyroglutamyl amino peptidase II activity and mRNA levels in rat limbic regions. PMID: 15707699

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Subcellular Location Secreted.
Protein Families TRH family
Database Links

KEGG: rno:25569

STRING: 10116.ENSRNOP00000015944

UniGene: Rn.22

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