Rat thyrotropin-releasing hormone,TRH ELISA KIT

Code CSB-E08040r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01150
Lead Time 3-5 working days
Abbreviation TRH
Target Name thyrotropin-releasing hormone
Alias MGC125964, MGC125965,
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.5 pg/mL-100 pg/mL
Sensitivity 0.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
HGNC 23747
RGD 1309303
MGI 1914510
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 100  
Range % 96-104  
1:2 Average % 92  
Range % 88-95  
1:4 Average % 93  
Range % 87-96  
1:8 Average % 101  
Range % 97-105  
Recovery
The recovery of rat TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 97 93-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
100 0.150 0.146 0.148    
25 0.301 0.298 0.300    
8 0.487 0.500 0.494    
2 0.776 0.790 0.783    
0.5 1.264 1.288 1.276    
0 1.932 1.907 1.920    
References
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Function Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.
Subcellular Location Secreted
Protein Families TRH family
Database Links

KEGG: rno:25569

STRING: 10116.ENSRNOP00000015944

UniGene: Rn.22

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