Human S100 calcium binding protein A9/calgranulin B,S100A9 ELISA Kit

Code CSB-E11834h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
S100 calcium binding protein A9
Alternative Names
Leukocyte L1 complex heavy chain ELISA Kit; 60B8AG ELISA Kit; CAGB ELISA Kit; Calgranulin B ELISA Kit; Calgranulin-B ELISA Kit; Calprotectin L1H subunit ELISA Kit; CFAG ELISA Kit; CGLB ELISA Kit; Cystic fibrosis antigen B ELISA Kit; L1AG ELISA Kit; Leukocyte L1 complex heavy chain ELISA Kit; LIAG ELISA Kit; MAC387 ELISA Kit; MIF ELISA Kit; Migration inhibitory factor related protein 14 ELISA Kit; Migration inhibitory factor-related protein 14 ELISA Kit; MRP 14 ELISA Kit; MRP-14 ELISA Kit; MRP14 ELISA Kit; Myeloid-related protein 14 ELISA Kit; NIF ELISA Kit; OTTHUMP00000015331 ELISA Kit; p14 ELISA Kit; Protein S100-A9 ELISA Kit; S100 A9 ELISA Kit; S100 calcium binding protein A9 ELISA Kit; S100 calcium binding protein A9 calgranulin B ELISA Kit; S100 calcium-binding protein A9 ELISA Kit; S100A9 ELISA Kit; S10A9_HUMAN ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.69 ng/mL-300 ng/mL
1.17 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human S100A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %100
Range %95-105
1:2Average %99
Range %94-102
1:4Average %94
Range %90-99
1:8Average %95
Range %88-98
The recovery of human S100A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9086-95
EDTA plasma (n=4)9692-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
3002.615 2.556 2.586 2.445
1501.841 1.784 1.813 1.672
751.263 1.251 1.257 1.116
37.50.757 0.739 0.748 0.607
18.750.461 0.445 0.453 0.312
9.380.288 0.273 0.281 0.140
4.690.207 0.198 0.203 0.062
00.142 0.140 0.141  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This human S100A9 ELISA Kit is suitable for qualitatively determining human concentrations in serum, plasma, and tissue homogenates in vitro. S100A9 is a calcium-binding protein that can be found in cells of myeloid origin, such as monocytes, neutrophils, and dendritic cells. After secretion, S100A9 acts as a chemoattractant to recruit inflammatory cells into the surrounding microenvironment. Secreted S100A9 protein plays a role in creating a favorable environment for cancer growth. It was indicated that myeloid cell-producing S100A9 protein within primary cancers and metastatic sites promotes the accumulation of more myeloid cells, which facilitates the regulation of tumor progression and premetastatic niche formation at metastatic sites. S100A9 has been detected in infiltrating macrophages in rheumatoid arthritis and other inflammatory conditions.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human S100A9 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human S100A9 antibody. Biotin-labeled S100A9 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the S100A9 in the human samples. S100A9 binds to the biotinylated anti-S100A9 human monoclonal antibody. And the biotin on the biotinylated anti-S100A9 human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of S100A9 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

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Target Background

(From Uniprot)
S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Has transnitrosylase activity; in oxidatively-modified low-densitity lipoprotein (LDL(ox))-induced S-nitrosylation of GAPDH on 'Cys-247' proposed to transfer the NO moiety from NOS2/iNOS to GAPDH via its own S-nitrosylated Cys-3. The iNOS-S100A8/A9 transnitrosylase complex is proposed to also direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif.
Gene References into Functions
  1. Acute exercise in children with juvenile idiopathic arthritis induced slightly musculoskeletal leg pain and transient increased plasma calprotectin levels but not IL-6 levels PMID: 30008613
  2. neither the inflammatory biomarker calprotectin, nor the tubular biomarkers NGAL and KIM-1, provide robust prognostic information on the loss of renal function in a heterogeneous CKD population. All of them, however, are candidate prognostic biomarkers in primarily inflammatory renal diseases. PMID: 30078006
  3. Serum levels of MRP8/MRP14 and MRP6 were up-regulated in patients with Graves' disease (GD) and Hashimoto's thyroiditis (HT). In addition, mRNA expression of MRP proteins in PBMCs and the thyroid gland was markedly elevated in these patients. PMID: 29656212
  4. High MRP14 expression is associated with Rheumatoid Arthritis PMID: 29530998
  5. upper limits for normal calprotectin values for neonatal, 6, 12 and 24 months old children, were determined. PMID: 29283308
  6. plasma calprotectin was investigated as a disease marker in patients with chronic RA treated with methotrexate (MTX) monotherapy and compared plasma calprotectin with C-reactive protein. PMID: 29228799
  7. Calprotectin measured in plasma showed the strongest associations with assessments of disease activity, and ethylenediaminetetraacetic acid-plasma should preferably be used when evaluating disease activity in rheumatoid arthritis patients. PMID: 29278951
  8. RAS is a very common inflammatory ulcerative condition of the oral cavity and its etiology is uncertain. Regarded as an inflammatory mechanism, releasing a high level of calprotectin in saliva has been suggested that it may play a role in pathogenesis of RAS. PMID: 29519972
  9. Pro-inflammatory proteins S100A9 and tumor necrosis factor-alpha suppress erythropoietin elaboration in myelodysplastic syndromes PMID: 28983059
  10. An immunohistochemical investigation revealed that patients with both UC and CD had higher neutrophil and monocyte/macrophage calprotectin-positive cell expression levels, compared with those in the normal controls. Fecal calprotectin was considered a reliable marker for disease activity, and the assessment of fecal calprotectin via POCT showed potential as a rapid and simple measurement in clinical settings. PMID: 29115397
  11. To examine the efficacy of faecal calprotectin (FC) and C-reactive protein (CRP) as predictors of small bowel capsule endoscopy SBCE findings in suspected and known Crohn's disease. PMID: 29466790
  12. S100A9, mainly produced by myeloid cells in the BM, is involved in the pathogenesis of MM. S100A9 attracted MM cells to the BM where it provided a survival niche by inducing the growth factors IL6 and IL10, as well as promoting angiogenesis. PMID: 28903971
  13. Study identified S100A9 protein to be up-regulated protein in hepatocellular carcinoma (HCC) and demonstrated its potentiality as a candidate for HCC diagnosis. PMID: 27216119
  14. In pediatric inflammatory bowel disease treatment groups, a significant inverse association was found between the quality of life and the levels of fecal calprotectin. PMID: 28169974
  15. Fecal calprotectin (FC) was elevated in two-thirds of stool specimens of patients with cystic fibrosis (CF). Increased FC was more common in patients with pancreatic insufficiency. Whether increased FC reflects intestinal inflammation in patients with CF remains to be determined. PMID: 28207476
  16. Authors found that downregulation of S100A9 significantly reduced the expression of NF-kappaB, phosphorylation of NF-kappaB and Bcl-2, as well as the expression of MMP7 and MMP2. Restoration of NF-kappaB expression sufficiently reversed the inhibitory effects on cell proliferation and invasion induced by S100A9 downregulation in vitro and in vivo. PMID: 28276321
  17. Urinary NGAL is a good predictor of mortality in established pediatric acute kidney injury (AKI) of heterogeneous etiology and outperforms calprotectin and KIM-1. Urinary calprotectin and KIM-1 predict renal replacement therapy requirement in pediatric AKI. PMID: 28409285
  18. Identify proteins that interact with calgranulin B to suppress the proliferation of colon cancer cells. PMID: 28036279
  19. This study demonstrated that S100A9 was detected in the serums of Alzheimer disease patients PMID: 27911324
  20. Expression levels of S100A9 and Tenascin-C were correlated with TNM stages and metastasis in colorectal cancer. PMID: 27191989
  21. In Guatemalan preschoolers, the median fecal calprotectin value was 58 mg/kg, with a mean of 98 +/- 136 mg/kg and a range from 10 to 950 mg/kg; 61% of values were above the manufacturer's cut-off for elevated concentration and 51% exceeded an age-adjusted criterion. The central tendency (mean or median) and distribution were generally shifted to the right in relation to comparable reports from children across the world. PMID: 27101538
  22. Low S100A9 expression is associated with head and neck neoplasms. PMID: 26883112
  23. Calprotectin had the highest association with ultrasound synovitis and predicted treatment response. It may thus be considered as a marker for evaluating inflammation and responsiveness in patients with rheumatoid arthritis on biologic disease-modifying anti-rheumatic drug treatment. PMID: 28081709
  24. Data support a role of S100A9 to initiate and amplify the neutrophilic inflammation in asthma, possibly via inducing IL-1beta, IL-17 and IFN-gamma. PMID: 28847516
  25. The median faecal calprotectin level was similar in cases with abdominal pain-related functional gastrointestinal disorders (7.8 mug/g, 95% CI: 7.8-8.4) including those with gastritis, and controls (9.1 mug/g, 95% CI: 7.8-11.3). Faecal calprotectin is not a predictor of gastritis and is similar in children with functional abdominal pain symptoms and in controls. PMID: 26913755
  26. S100A9 induces anti-apoptotic effect on normal and allergic neutrophils by increasing cytokine secretion of monocytes PMID: 27459393
  27. S100A9, a protein previously described to be involved in modulation of inflammatory response, was found to interact with schistosoma MEG-14. PMID: 27639541
  28. High calprotectin activity could be demonstrated within the lumen of vermiform appendix specimens following appendectomy for acute appendicitis. PMID: 27118309
  29. observations indicate a novel way in which S100A9 may contribute to the pathogenesis of gout, by priming neutrophils to respond to MSUs. PMID: 28550118
  30. S100A9 was an ATRA-responsive gene, and PML/RARalpha was necessary for the ATRA-induced expression of S100A9 in acute promyelocytic leukemia (APL) cells. PML/RARalpha and PU.1 were necessary for the ATRA-induced expression of S100A9 in APL cells. Furthermore, S100A9 promoted apoptosis in APL cells and affected cell growth. PMID: 28063140
  31. Salivary S100A9 is a biomarker for oral squamous cell carcinoma in a Hungarian population. PMID: 28545132
  32. High S100A9 expression is associated with glioblastoma. PMID: 27006175
  33. Faecal calprotectin and CRP are predictive of symptomatic relapse and may be valuable in management of paediatric IBD in clinical remission PMID: 28138990
  34. These results suggest a preponderant role for the protein S100-A9 as an activator of the NF-kappa B pathway during Chronic lymphocytic leukemia progression and suggest that the leukemic clone can generate an autoactivation loop through S100-A9 expression, NF-kappa B activation, and exosome secretion. PMID: 28596424
  35. Fecal calprotectin levels were increased in pediatric subjects who showed signs of severe Clostridium difficile infection (CDI). Increased serum calprotectin levels were associated with increased disease severity in 48 adults with CDI PMID: 27668938
  36. High S100A9 expression is associated with lung metastasis in malignant melanoma. PMID: 27151304
  37. A significant positive correlation was found between the serum calprotectin and insulin resistance in polycystic ovary syndrome patients. PMID: 27217417
  38. these results indicate that CD14 is a co-receptor of TLR4 in the S100A9-induced cytokine response. PMID: 27228163
  39. Our data reveals that there is no significant correlation between serum calprotectin and Faecal calprotectin , nor between serum calprotectin and CRP in a large unselected cohort of GI patients. PMID: 28093196
  40. The present study demonstrates the potential of calprotectin to distinguish Rheumatoid Arthritis patients in both clinical and ultrasound-defined remission from patients in clinical remission but with residual subclinical disease activity. PMID: 27832086
  41. S100A9 and S100A12 may have a role in the pathogenesis of pneumonia: S100A9 and CXCL1 may contribute solely in mild pneumonia, and CCL5 and CXCL11 may contribute in severe pneumonia. PMID: 28381820
  42. patients with primary Sjogren's syndrome have a higher prevalence of carotid atherosclerosis, which is associated with higher serum calprotectin level independent of traditional cardiovascular risk factors. PMID: 27749218
  43. Serum calprotectin was significantly increased in inflammatory bowel disease compared with controls. PMID: 27596694
  44. Urinary calprotectin is a promising biomarker for the differentiation of prerenal and intrinsic acute renal allograft failure. PMID: 26901081
  45. Faecal biomarkers lactoferrin, calprotectin, and PMN-elastase were able to distinguish between ulcerative colitis patients with mucosal healing from clinical remission and mild disease, showed significant correlations with endoscopy, and were predictive of a flare. PMID: 26874351
  46. Blocking the interaction between S100A9 and RAGE V domain is a novel route to drug development against cell proliferation. PMID: 27524699
  47. The minor allele (T) of the S100A9 variant rs3014866 is associated with lower type 2 diabetes risk in 3 populations of different ancestries. PMID: 27440084
  48. fecal calprotectin can be used in management of patients with inflammatory bowel disease PMID: 27688663
  49. this paper shows that human gammadelta T cell-antigen-presenting cells stimulate CD4+ T cell responses distinct from those induced by myeloid antigen-presenting cells to promote local barrier defense via mucosal release of IL-22 and calprotectin PMID: 28330898
  50. The calprotectin levels in patients with atrial fibrillation were significantly higher than in healthy blood donors and were further increased after cardioversion. PMID: 28276729

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Subcellular Location
Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein.
Protein Families
S-100 family
Tissue Specificity
Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes
Database Links

HGNC: 10499

OMIM: 123886

KEGG: hsa:6280

STRING: 9606.ENSP00000357727

UniGene: Hs.112405

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